Skip to main content
  • Letter
  • Published:

5HT2A polymorphism His452Tyr in a German Caucasian systemic sclerosis population – authors' response

The variance of results between our recent article [1] and the replication study by Kirsten and colleagues [2] in German patients may prompt the reader to ask the simple question: what conclusion can be drawn from these contradictory results?

One possible answer is that one of the two studies is wrong and all the clues would indicate the first report as the one describing a spurious finding. This is consistent with a general overestimate of the role of a gene in the first association study of it [3] and, indeed, we have always been aware of the possibility of false positive findings. Hence, we provided the reader a mean (for example, the false probability report probability) with which to gauge the strength of the association and the chance of having described a spurious result [1].

A second possible answer is that the variance of results stems from methodological differences between the two studies. It has been argued that replication studies should be viewed as tools to uncover heterogeneity rather than tools to test the hypothesis generated by the initial association [3] and, in a more extreme view, the relevance of replication has been questioned [4]. Our and Kirsten and colleagues' studies are heterogeneous with regard to many aspects, starting with 'phenotype definition'. In our report we included only patients fulfilling the American College of Rheumatology criteria for the classification of systemic sclerosis (SSc), while Kristen and colleagues also included 19% of early SSc patients according to LeRoy's definition [5]; we also had 75% limited cutaneous SSc patients compared to 50% in the German population. This different phenotype definition implies that the sets of causative loci or traits underlying the different definitions are likely to be different [3]. Moreover, the prevalence of females in our population was much higher (93.9% versus 50%) and it is well-known that platelets differently aggregate in males and females due to hormonal and gender-specific influences [6]. As we hypothesized that the rs6314 polymorphism does not act as a causative genetic mutation per se, but rather it is important in amplifying SSc-causative and coagulative pathological processes once they have already been triggered by other factors [1], all the above-mentioned and other hidden differences in the genetic background are likely to be of particular relevance. In this sense, it is reductive to consider the importance of the studied single nucleotide polymorphism on SSc susceptibility only for its main independent effect, but it should more appropriately be viewed in the context of a broader gene-gene or gene-environmental frame.



systemic sclerosis.


  1. Beretta L, Cossu M, Marchini M, Cappiello F, Artoni A, Motta G, Scorza R: A polymorphism in the human serotonin 5-HT2A receptor gene may protect against systemic sclerosis by reducing platelet aggregation. Arthritis Res Ther. 2008, 10: R103-10.1186/ar2495.

    Article  PubMed Central  PubMed  Google Scholar 

  2. Kirsten H, Burkhardt J, Hantmann H, Hunzelmann N, Vaith P, Ahnert P, Melchers I: 75HT2A polymorphism His452Tyr in a German Caucasian systemic sclerosis population. Arthritis Res Ther. 2009, 11: 403-

    Article  PubMed Central  PubMed  Google Scholar 

  3. Sillanpää MJ, Auranen K: Replication in genetic studies of complex traits. Ann Hum Genet. 2004, 68: 646-657. 10.1046/j.1529-8817.2004.00122.x.

    Article  PubMed  Google Scholar 

  4. Vieland VJ: The replication requirement. Nat Genet. 2001, 29: 244-245. 10.1038/ng1101-244.

    Article  CAS  PubMed  Google Scholar 

  5. LeRoy EC, Medsger TA: Criteria for the classification of early systemic sclerosis. J Rheumatol. 2001, 28: 1573-1576.

    CAS  PubMed  Google Scholar 

  6. Haque SF, Matsubayashi H, Izumi S, Sugi T, Arai T, Kondo A, Makino T: Sex difference in platelet aggregation detected by new aggregometry using light scattering. Endocr J. 2001, 48: 33-41. 10.1507/endocrj.48.33.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Raffaella Scorza.

Additional information

Competing interests

The authors declare that they have no competing interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Beretta, L., Scorza, R. 5HT2A polymorphism His452Tyr in a German Caucasian systemic sclerosis population – authors' response. Arthritis Res Ther 11, 404 (2009).

Download citation

  • Published:

  • DOI: