- Open Access
B cells, BAFF/zTNF4, TACI, and systemic lupus erythematosus
Arthritis Research & Therapy volume 3, Article number: 197 (2001)
B cells and B-cell/T-cell collaborations are instrumental in the pathophysiology of systemic lupus erythematosus (SLE). This commentary highlights in particular the newly discovered role of B-cell-activating factor (BAFF; also known as TALL-1, THANK, BlyS, and zTNF4) as a positive regulator of B-cell functions, such as B-cell activation and differentiation. Two members of the tumor necrosis factor(TNF)-receptor superfamily were recently identified as receptors for BAFF on B cells. The interaction between BAFF and its receptors may be important in the pathogenesis of lupus. Advances in our understanding of abnormalities in immune regulation in lupus might provide the opportunity to improve our current therapeutic approaches to this disorder.
B cells are believed to be central to the pathogenesis of SLE. Specific patterns of autoantibodies are characteristically detected in lupus; in addition, a direct pathogenic role of these autoantibodies has been convincingly demonstrated for at least some, such as anti-dsDNA, anti-Ro, and anti-cardiolipin antibodies. Besides secreting pathogenic antibodies, recent studies have shed important new light on the role of B cells in shaping the immune repertoire, thereby pointing to new ways in which B cells may contribute to the pathogenesis of lupus. In contrast to mice deficient in T-cell receptors, mice lacking B cells do not develop M cells in the gut, suggesting that B cells are involved in the organogenesis of gut-associated lymphoid tissue . Since M cells can function as professional antigen-presenting cells, it was unexpected that B cells play a 'directing' role during the development of these cells. An important murine model for lupus is the MRL-lpr/lpr mouse, characterized by Fas deficiency, double negative T cells, autoantibodies, vasculitis, and glomerulonephritis. Chan et al  showed that MRL-lpr/lpr mice deficient in antibody secretion still develop nephritis and vasculitis. This finding challenged the standard view that the major contribution of B cells in promotion of autoimmunity in murine lupus is by antibody production, and led to the speculation that B cells may play a promoting role as antigen-presenting cells in the course of the disease. Chan et al suggested that their findings are consistent with the presence of an amplification loop between cognate B and T cells, resulting in an increase of memory and effector T cells . This latter interpretation is consistent with a recent study by David Gray and colleagues  demonstrating that TH cell memory depends on the presence of B cells but is clearly independent of the presentation of peptides by these B cells. Further studies [4,5] have found that IgM-deficient mice develop autoimmune features suggestive of lupus, including the production of anti-dsDNA antibodies. Since a similar autoimmune tendency has been reported in human patients deficient for IgA , it is conceivable that immunoglobulins are also instrumental in self-regulation. Therefore, it appears that we are just beginning to understand an integrated network of different immune-cell compartments where B cells seem to be of more central importance than was previously appreciated.
A consistent finding in lupus is intrinsic B-cell hyperreactivity. Upon stimulation of the B-cell receptor, lupus B cells show abnormally high Ca influxes followed by higher concentrations of inositol triphosphate and tyrosine phosphorylated proteins, as compares to B cells from normal controls , indicating a unique, intrinsic abnormality of B cells in SLE. However, an overwhelming B-cell overactivity induced by signaling through membrane receptors cannot be excluded. In this context, stimulation via complement receptor 2 has been suggested to contribute to signaling abnormalities in lupus , since the ligand of this receptor, C3d, was identified to be part of immune complexes in lupus .
Anti-dsDNA antibodies present in SLE are generally IgG with high affinity for antigen, and display somatic mutations in the immunoglobulin variable regions. These are molecular characteristics of antibodies arising in an antigen-driven, T-cell-dependent response. Furthermore, blocking B-cell/T cell costimulation with CTLA4Ig or anti-CD40 ligand in murine lupus results in dramatic effects on anti-DNA antibody titers, renal disease, and survival [10,11,12,13,14]. Clearly, B-cell/T-cell cognate interactions are critical in lupus; inhibition of costimulation is a novel and potentially very useful approach to the treatment of human autoimmune disease.
BAFF/zTNF and TACI, a novel ligand/receptor pair
Interactions between tumor necrosis factor (TNF)-like ligands and their receptors are crucial to the regulation of the immune response, via induction of apoptosis or by promoting cell survival and proliferation . The recent discovery of interacting molecules belonging to these ever-growing families has afforded important insights into normal and pathological immunity, while facilitating the development of a new approach to therapeutic modulation of autoimmune disease by blocking a novel pathway of Band T-cell interaction.
BAFF (B-cell-activating factor) was identified as a member of the TNF family in 1999 by several independent research groups and consequently is alternatively referred to in the literature as TALL-1, THANK, BlyS, and zTNF4 [16,17,18,19]. BAFF is expressed on dendritic cells, monocytes/macrophages, and T cells. It quickly became clear that BAFF is a positive regulator of B-cell function, with effects on cell survival, activation, and differentiation. Soluble BAFF costimulates B cells activated by anti-IgM  or by IL-4 , and may also have weaker direct stimulatory effects .
By means of receptor-cloning methodology, two previously orphan members of the TNF-receptor superfamily, known as TACI (transmembrane activator and CAML-interactor) and BCMA (B-cell-maturation antigen), were found to be the receptors for BAFF on B cells [21,22,23,24]. Soluble receptor (TACI-Ig: a fusion protein of the extracellular domain of the receptor with the Fc portion of an immunoglobulin molecule) prevented binding of BAFF to B cells and inhibited its stimulatory effect on human and murine B cells in vitro . Blocking the interaction of BAFF receptor with TACI-Ig in immunized mice results in significantly decreased numbers of antigen-specific IgM and IgG antibodies. Moreover, treated mice were found to be devoid of germinal centers . These data and others suggest that the interaction between BAFF and TACI, in an analogous fashion to the related costimulatory pair CD40 ligand/CD40, is crucial for the normal development of antigen-specific humoral responses. In contrast to mice treated with TACI-Ig, however, mice deficient in CD40 ligand and CD40 have normal IgM responses, suggesting that the interaction between BAFF and TACI may occur earlier in B-cell activation. It is important to note that soluble TACI also inhibits T-cell-independent responses . More recent studies have addressed the molecular mechanisms involved in BAFF binding to its receptor [21,23,26]. Similar to other members of the TNF-receptor family that lack a death domain , the intracellular domain of TACI associates with TNF-receptor-associated factors (TRAF) and activates NFκB and c-Jun NH2 terminal kinase, thus presumably transducing signals for B-cell proliferation and survival.
BAFF and autoimmunity
The discovery that BAFF mediates a strong signal for B-cell proliferation and survival led researchers to consider a role for this molecule in diseases characterized by B-cell hyperactivity. Important confirmation of a possible role of BAFF in the pathogenesis of autoimmunity came from transgenic studies. In BAFF-transgenic mice (under control of a liver specific promotor), Mackay et al  found a significant expansion of the peripheral B-cell compartment (spleen and lymph nodes), with mature cells exhibiting an activated phenotype. Numerous germinal centers were present without immunization, and the number of plasma cells was increased. Some but not all BAFF-transgenic mice displayed marked hyperglobulinema, with circulating immune complexes and a positive rheumatoid factor, high titers of antibodies against single-stranded and double-stranded DNA, and immunoglobulin deposition in the kidneys. Similar results were reported by Khare et al , who generated TALL-1 transgenic mice under the control of a beta actin promoter. The transgenic mice had marked B-cell hyperplasia and hypergamma-globulinemia. Furthermore, there was a progressive increase in serum IgM and IgG anti-dsDNA antibody titers over time, followed by immune-complex nephritis and renal dysfunction. The serological manifestations and renal involvement in both BAFF transgenic models are strongly reminiscent of SLE. Moreover, high serum concentrations of BAFF were found in two murine models of lupus (NZB × NZW F1 and MRL-lpr/lpr), with an increase in serum BAFF concentrations over time paralleling worsening disease activity .
The positive regulatory effects of BAFF on B cells and on T-cell-dependent humoral responses, the autoimmune phenotype of BAFF transgenic mice, and the high levels of BAFF in lupus-prone mice suggest that blocking the interaction between BAFF and its receptor with TACI-Ig may be a useful therapeutic approach in murine lupus . Female 21-week-old B/W mice were treated with TACI-Ig three times weekly for 5 weeks. Mice treated with a high dose of TACI-Ig had a markedly delayed onset of proteinuria. Importantly, treatment also resulted in a meaningful difference in survival, with 100% of the animals receiving TACI-Ig surviving to 38 weeks versus only 47% of controls. Although anti-dsDNA antibody titers did not decline, treated mice exhibited a decrease in peripheral-blood B cells.
Clearly, we do not yet fully appreciate the myriad immune defects found in SLE. In this commentary, we discussed some of the recent experimental approaches to defining novel roles for B cells and B-cell/T-cell collaboration in the pathophysiology of SLE. These discoveries have provided new insights into our understanding of abnormalities in immune regulation in lupus, and might eventually provide the opportunity to improve the specificity and effectiveness of therapeutic approaches to this complex disease.
systemic lupus erythematosus
transmembrane activator and CAML-interactor
tumor necrosis factor
Golovkina TV, Shlomchik M, Hannum L, Chervonsky A: Organogenic role of B lymphocytes in mucosal immunity. Science. 1999, 286: 1965-1968. 10.1126/science.286.5446.1965.
Chan OT, Hannum LG, Haberman AM, Madaio MP, Shlomchik MJ: A novel mouse with B cells but lacking serum antibody reveals an antibody-independent role for B cells in murine lupus. J Exp Med. 1999, 189: 1639-1648. 10.1084/jem.189.10.1639.
van Essen D, Dullforce P, Brocker T, Gray D: Cellular interactions involved in Th cell memory. J Immunol. 2000, 165: 3640-3646.
Ehrenstein MR, Cook HAT, Neuberger MS: Deficiency in serum immunoglobulin (Ig)M predisposes to development of IgG autoantibodies. J Exp Med. 1999, 191: 1253-1257. 10.1084/jem.191.7.1253.
Boes MT, Schmidt T, Linkemann K, Beaudette BC, Marshak-Rothstein A, Chen J: Accelerated development of IgG autoantibodies and autoimmune disease in the absence of secreted IgM. Proc Natl Acad Sci USA. 2000, 97: 1184-1189. 10.1073/pnas.97.3.1184.
Rankin EC, Isenberg DA: IgA deficiency and SLE: prevalence in a clinical population and a review of the literature. Lupus. 1997, 6: 390-394.
Liossis SN, Kovacs B, Dennis G, Kammer GM, Tsokos GC: B cells from patients with systemic lupus erythematosus display abnormal antigen receptor-mediated early signal transduction events. J Clin Invest. 1996, 98: 2549-2557.
Tsokos GC, Wong HK, Enyedy EJ, Nambiar MP: Immune cell signaling in lupus. Curr Opin Rheumatol. 2000, 12: 355-363. 10.1097/00002281-200009000-00001.
Aguado MT, Lambris JD, Tsokos GC, Burger R, Bitter-Suermann D, Tamerius JD, Dixon FJ, Theofilopoulos AN: Monoclonal antibodies against complement 3 neoantigens for detection of immune complexes andcomplement activation. Relationship between immune complex levels, state of C3, and numbers of receptors for C3b. J Clin Invest. 1985, 76: 1418-1426.
Mackay F, Woodcock SA, Lawton P, Ambrose C, Baetscher M, Schneider P, Tschopp J, Browning JL: Mice transgenic for BAFF develop lymphocytic disorders along with autoimmune manifestations. J Exp Med. 1999, 190: 1697-1710. 10.1084/jem.190.11.1697.
Finck BK, Linsley PS, Wofsy D: Treatment of murine lupus with CTLAIg. Science. 1994, 265: 1225-1227.
Mihara M, Tan I, Chuzhin Y, Reddy B, Budhai L, Holzer A, Gu Y, Davidson A: CTLA4Ig inhibits T cell-dependent B-cell maturation in murine systemic lupus erythematosus. J Clin Invest. 2000, 106: 91-101.
Mohan C, Shi Y, Laman JD, Datta SK: Interaction between CD40 and its ligand gp39 in the development of murine lupus nephritis. J Immunol. 1995, 154: 1470-1480.
Early GS, Zhao W, Burns CM: Anti-CD40 ligand antibody treatment prevents the development of lupus-like nephritis in a subset of New Zealand Black × New Zealand White mice. J Immunol. 1996, 157: 3159-3164.
Laabi Y, Strasser A: Lymphocyte survival - ignorance is Blys. Science. 2000, 289: 883-884. 10.1126/science.289.5481.883.
Schneider P, MacKay F, Steiner V, Hofmann K, Bodmer JL, Holler N, Ambrose C, Lawton P, Bixler S, Acha-Orbea H, Valmori D, Romero P, Werner-Favre C, Zubler RH, Browning JL, Tschopp J: BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J Exp Med. 1999, 189: 1747-1756. 10.1084/jem.189.11.1747.
Shu HB, Hu WH, Johnson H: TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol. 1999, 65: 680-683.
Mukhopadhyay A, Ni J, Zhai Y, Yu GL, Aggarwal BB: Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase. J Biol Chem. 1999, 274: 15978-15981. 10.1074/jbc.274.23.15978.
Moore PA, Belvedere O, Orr A, Pieri K, LaFleur DW, Feng P, Soppet D, Charters M, Gentz R, Parmelee D, Li Y, Galperina O, Giri J, Roschke V, Nardelli B, Carrell J, Sosnovtseva S, Greenfield W, Ruben SM, Olsen HS, Fikes J, Hilbert DM: BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator. Science. 1999, 285: 260-263. 10.1016/S0925-8388(98)00943-8.
Khare SD, Sarosi I, Xia XZ, McCabe S, Miner K, Solovyev I, Hawkins N, Kelley M, Chang D, Van G, Ross L, Delaney J, Wang L, Lacey D, Boyle WJ, Hsu H: Severe B cell hyperplasia and autoimmune disease in TALL-1 transgenic mice. Proc Natl Acad Sci USA. 2000, 97: 3370-3375. 10.1073/pnas.050580697.
Yan M, Marsters SA, Grewal IS, Wang H, Ashkenazi A, Dixit VM: Identification of a receptor for Blys demonstrates a crucial role in humoral immunity. Nature Immunol. 2000, 1: 37-40. 10.1038/76889.
Thompson JS, Schneider P, Kalled SL, Wang L, Lefevre EA, Cachero TG, MacKay F, Bixler SA, Zafari M, Liu ZY, Woodcock SA, Qian F, Batten M, Madry C, Richard Y, Benjamin CD, Browning JL, Tsapis A, Tschopp J, Ambrose C: BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population. J Exp Med. 2000, 192: 129-135. 10.1084/jem.192.1.129.
Xia XZ, Treanor J, Senaldi G, Khare SD, Boone T, Kelley M, Theill LE, Colombero A, Solovyev I, Lee F, McCabe S, Elliott R, Miner K, Hawkins N, Guo J, Stolina M, Yu G, Wang J, Delaney J, Meng SY, Boyle WJ, Hsu H: TACI is a TRAF-interacting receptor for TALL-1, a tumor necrosis factor family member involved in B cell regulation. J Exp Med. 2000, 192: 137-143. 10.1084/jem.192.1.137.
Johnson H, Shu HB: B cell maturation protein is a receptor for the tumor necrosis factor family member TALL-1. Proc Natl Acad Sci USA. 2000, 97: 9156-9161. 10.1073/pnas.160213497.
Gross JA, Johnston J, Mudri S, Enselman R, Dillon SR, Madden K, Xu W, Parrish-Novak J, Foster D, Lofton-Day C, Moore M, Littau A, Grossman A, Haugen H, Foley K, Blumberg H, Harrison K, Kindsvogel W, Clegg CH: TACI and BCMA are receptors for a TNF homologue implicated in B-cell autoimmune disease. Nature. 2000, 404: 995-999. 10.1038/35010115.
Hatzoglou A, Roussel J, Bourgeade MF, Rogier E, Madry C, Inoue J, Devergne O, Tsapis A: TNF receptor family member BCMA (B cell maturation) associates with TNF receptor-associated factor (TRAF) 1, TRAF2, and TRAF3 and activates NF-kappa B, elk-1, c-Jun N-terminal kinase, and p38 mitogen-activated protein kinase. J Immunol. 2000, 165: 1322-1330.
About this article
Cite this article
Dörner, T., Putterman, C. B cells, BAFF/zTNF4, TACI, and systemic lupus erythematosus. Arthritis Res Ther 3, 197 (2001). https://doi.org/10.1186/ar299
- TNF receptor superfamily