Volume 3 Supplement 1

Innovative Rheumatology: Gene and Cell Therapies of Arthritis and Related Autoimmune Disorders. Second International Meeting

Open Access

Local IL-17 gene therapy accelerates collagen arthritis with severe bone erosion and rank ligand and rank expression in synovial infiltrate and at bone erosion sites

  • E Lubberts1,
  • LAB Joosten1,
  • P Schwarzenberger2,
  • B Oppers1,
  • L van de Bersselaar1,
  • JK Kolls2 and
  • WB van den Berg1
Arthritis Research & Therapy20013(Suppl 1):P20

https://doi.org/10.1186/ar346

Received: 6 April 2001

Published: 25 April 2001

Objective

To examine the effects of local IL-17 application in the knee joint of type II collagen immunized mice on the induction of bone erosion.

Method

Collagen-induced arthritis (CIA) was induced in male DBA-1 mice by immunizing intradermally at the base of the tail with suboptimal dose of bovine type II collagen. On day 21, mice were given an intraperitoneal booster injection of the same dose of type II collagen dissolved in PBS. Just before expected onset, mice were intra-articularly injected into the right knee joint with 107 pfu of either an IL-17 expressing (AdIL-17) or control (AdControl) recombinant human type 5 adenovirus vector. Five days after the intra-articular injection of the viral vector arthritis was monitored visually and joint pathology was examined by histology. Formation of osteoclast-like cells was determined by tartrate-resistant acid phosphatase (TRAP) staining. In addition, RANKL and RANK protein expression was evaluated by specific immunohistochemistry.

Results

Local IL-17 over-expression in the knee joint of type II collagen immunized mice promotes synovial inflammation. Five days after viral injection of AdIL-17 histologic analysis showed aggravation of bone erosion in the patella and femur/tibia region compared with the control vector group. Induction of bone destruction by IL-17 was accompanied with marked tartrate-resistant acid phos-phatase (TRAP) activity in the bone marrow and at bone erosion sites, indicating that IL-17 accelerates the formation of osteoclast-like cells. Interestingly, local IL-17 promotes local protein expression of RANKL and its receptor RANK in the synovial infiltrate and at bone erosion sites compared with the control vector group.

Conclusion

These data show that local IL-17 gene therapy during onset of collagen arthritis promotes osteoclastic bone erosion accompanied with accelerated expression of local RANKL and its receptor RANK. These findings suggest IL-17 to be a potent stimulator of osteoclastogenesis during arthritis.

Authors’ Affiliations

(1)
UMC St Radboud, Rheumatology Research Laboratory
(2)
Gene Therapy Program, Louisiana State University

Copyright

© BioMed Central Ltd 2001

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