Skip to main content
  • Poster presentation
  • Open access
  • Published:

Effect of B cell depletion using peptide tetramers in collagen-induced arthritis


B cell depletion therapy is effective in the treatment of various autoimmune diseases. However, this therapy is shown to be associated with increased risk of adverse effects such as opportunistic infections. Therefore, in this study, we developed and analyzed the selective depletion therapy of pathogenic B cells using peptide tetramers in collagen-induced arthritis model.


Since the antigenic targets of pathogenic antibodies are identified in collagen-induced arthritis (CIA) model, we developed toxin-conjugated peptide tetramers, which contained pathogenic epitope of mouse type II Collagen (CII). The male DBA/1J mice were immunized with bovine CII and injected with toxin-conjugated peptide tetramers on day 10 and day 20 after CIIimmunization.We analyzed the effect of toxin-conjugated peptide tetramers on the production of autoantibodies and clinical course of arthritis.


The incidence of arthritis was significantly lower (P < 0.05) in the tetramer-treated group than in the control group. The mean serum antibody levels for CII did not differ significantly, but there were significant differences in the anti-peptide antibodies over time.


Peptide tetramer is effective in the selective depletion of antigen-specific B cells and decreased the incidence of arthritis in CIA model. Therefore, depletion of antigen-specific B cells using this strategy might be a new therapeutic intervention of autoimmune diseases.

Author information

Authors and Affiliations


Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Cite this article

Michishita, K., Kawahata, K., Kanzaki, T. et al. Effect of B cell depletion using peptide tetramers in collagen-induced arthritis. Arthritis Res Ther 14 (Suppl 1), P33 (2012).

Download citation

  • Published:

  • DOI: