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Arthritis Research & Therapy

Open Access

Tks5-dependent formation of circumferential podosomes mediates cell-cell fusion

  • Tsukasa Oikawa1,
  • Masaaki Oyama2,
  • Hiroko Kozuka-Hata2,
  • Shunsuke Uehara3,
  • Nobuyuki Udagawa3,
  • Hideyuki Saya4, 5 and
  • Koichi Matsuo1
Arthritis Research & Therapy201214(Suppl 1):P55

Published: 29 February 2012

Multinucleation of osteoclasts during osteoclastogenesis requires dynamic rearrangement of the plasma membrane and cytoskeleton, and this process involves numerous previously characterized factors. However, the mechanism underlying osteoclast fusion remains obscure. Live-imaging analysis of osteoclastogenesis revealed that the products of PI3-kinase are enriched at the sites of osteoclast fusion. Among the downstream molecules whose expression was screened, the expression of Tks5, an adaptor protein with the phox homology (PX) domain with multiple Src homology 3 domains, was induced during osteoclastogenesis. Tks5 was localized in the podosomes and fusing membranes of osteoclasts, and reducing its expression impaired both formation of circumferential podosomes and osteoclast fusion without altering osteoclast differentiation. In addition, the expression of a deletion mutant of the PX domain abrogated circumferential podosome formation as well as osteoclast fusion, suggesting that Tks5-dependent circumferential podosomes function as fusion machinery during osteoclastogenesis. As Tks5 is known to promote the formation of podosomes/invadopodia in transformed/cancer cells, we tested if these cells also have the potential to fuse with osteoclasts. Among the cells tested, B16F0 melanoma cells formed circumferential podosomes with Tks5 accumulation in the presence of RANKL, TGFβ and TNFα. Co-culture of B16F0 melanoma cells with osteoclasts in an inflammatory milieu promoted increased formation of melanoma-osteoclast hybrid cells. Our results revealed a previously unknown mechanism of regulation of both circumferential podosome formation and cell-cell fusion by Tks5.

Authors’ Affiliations

Laboratory of Cell and Tissue Biology, Institute for Integral Medical Research, School of Medicine, Keio University, Shinjuku-ku, Tokyo, Japan
Medical Proteomics Laboratory, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan
Department of Biochemistry, Matsumoto Dental University, Hiro-oka, Shiojiri, Nagano, Japan
Division of Gene Regulation, Institute for Advanced Medical Research, School of Medicine, Keio University, Shinjuku-ku, Tokyo, Japan
CREST, Japan Science and Technology Agency, Tokyo, Japan


© Oikawa et al.; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.