Cartilage oligomeric matrix protein specific antibodies are pathogenic

Table 1 Summary of mAb specificities

Clones	Mice	Isotypes	Rat COMP	Mouse COMP	Human COMP	Binding sites
1A12	COMP^-/-	IgG1	++	++	_	EGF-like domain
1B8	COMP^-/-	IgG1	+	+	_	TSP3 domain
1D10	COMP^-/-	IgG1	++	++	++	C-terminal globular domain
2D3	COMP^-/-	IgG1	+++	+++	+++	coiled-coil domain or pentameric COMP
1C1	COMP^-/-	IgG2b	+	++	_	EGF-like domain
3C7	COMP^-/-	IgG2b	+	_	_	ND
3F8	COMP^-/-	IgG2b	++	++	_	EGF-like domain
4A8	COMP^-/-	IgG2b	++	++	_	C-terminal globular domain
8E3	COMP^-/-	IgG1	++	+	_	EGF-like domain
12H8	COMP^+/+	IgG1	+++	+++	_	EGF-like domain
15A11	COMP^+/+	IgG1	+++	+++	+++	EGF-like domain
4D12	COMP^-/-	IgG2b	+	_	_	EGF-like domain
3F11	COMP^-/-	IgG2a	++	+	_	EGF-like domain
16B5	COMP^+/+	IgG1	+++	+++	+++	coiled-coil domain or pentamer COMP
18E7	COMP^-/-	IgG2b	+	+	_	EGF-like domain
5E8	COMP^-/-	IgG2b	+	_	_	ND
6H4	COMP^-/-	IgG2b	++	++	+	EGF-like domain
29C3	COMP^-/-	IgG1	+	+++	_	EGF-like domain
30G5	COMP^-/-	IgG2b	++	++	_	C-terminal globular domain
23A8	COMP^-/-	IgG1	++	+++	_	EGF-like domain
24F7	COMP^-/-	IgG2b	++	++	_	EGF-like domain

Grading of values obtained with mAbs using culture supernatants. Scale for optical density (OD) values: -, < 3 times standard error of the mean (SEM) of negative control; +, > 3 times SEM of negative control; ++, > 0.5 OD; +++, > 1.0 OD; COMP, cartilage oligomeric matrix protein; EGF, epithelial growth factor; ND, not determined.

ISSN: 1478-6362