Supplementary Figure 4

Mixed lymphocyte culture of total (a) and CD4⁺(b) spleen T cells from normal Lewis rats with different densities of allogeneic (Wistar–Prob) stimulator cells, supplemented with isotype controls (IgG₁, IgG_2a), or the anti-CD4 mAbs W3/25, OX35, and RIB5/2 at 1 μg/ml. Data are expressed as means ± SEM of the relative absorbance from three independent experiments (triplicates in each experiment), normalized to the absorbance of PBS-treated cultures (=100%). ^*P ≤ 0.05 in comparison with the respective isotype control, ^#P ≤ 0.05 in comparison with W3/25, ^§P ≤ 0.05 in comparison with OX35.