Figure 2

Rheumatoid arthritis subjects have a lower proportion of IL-17A⁺but a higher proportion of IFNγ⁺CD4⁺T cells compared with controls. (A) Circulating T-helper type (Th)17 and Th1 cells. Peripheral blood mononuclear cells (PBMCs) from rheumatoid arthritis (RA) patients and healthy controls were gated on CD3⁺CD4⁺ lymphocytes and were analyzed for expression of interleukin (IL)-17A (Th17 cells) and interferon gamma (IFNγ; Th1 cells) (Mann–Whitney U test). (B) Circulating CD161⁺ and T_EM cells. PBMCs were gated on CD3⁺CD4⁺ lymphocytes and were analyzed for expression of CD161 and CD45RO (T_EM cells) (Mann–Whitney U test). (C) CD161⁺IL17A⁺ population. PBMCs were gated on CD3⁺CD4⁺CD161⁺ and analyzed for expression of IL-17A and IFNγ (Mann–Whitney U test). (D) T_EM IL-17A⁺ population. PBMCs were gated on CD3⁺CD4⁺CD45RO⁺ lymphocytes and were analyzed for expression of IL-17A and IFNγ (Mann–Whitney U test).