Skip to main content
Download PDF

Rheumatoid arthritis patients exhibit impaired Candida albicans-specific Th17 responses

Arthritis Research & Therapy volume 16, Article number: R50 (2014) Cite this article

Abstract

Introduction

Accumulating data implicate the CD4+ T cell subset (Th17 cells) in rheumatoid arthritis (RA). IL-17 is an inflammatory cytokine that induces tumor necrosis factor (TNF)α, IL-1β and IL-6, all of which are targets of biologic therapies used to treat RA. RA patients are well documented to experience more infections than age-matched controls, and biologic therapies further increase the risk of infection. The Th17/IL-17 axis is vital for immunity to fungi, especially the commensal fungus Candida albicans. Therefore, we were prompted to examine the relationship between RA and susceptibility to C. albicans because of the increasing interest in Th17 cells and IL-17 in driving autoimmunity, and the advent of new biologics that target this pathway.

Methods

We analyzed peripheral blood and saliva from 48 RA and 33 healthy control subjects. To assess C. albicans-specific Th17 responses, PBMCs were co-cultured with heat-killed C. albicans extract, and IL-17A levels in conditioned supernatants were measured by ELISA. The frequency of Th17 and Th1 cells was determined by flow cytometry. As a measure of IL-17A-mediated effector responses, we evaluated C. albicans colonization rates in the oral cavity, salivary fungicidal activity and levels of the antimicrobial peptide β-defensin 2 (BD2) in saliva.

Results

Compared to controls, PBMCs from RA subjects exhibited elevated baseline production of IL-17A (P = 0.004), although they had similar capacity to produce IL-17A in response to Th17 cell differentiating cytokines (P = 0.91). However RA PBMCs secreted less IL-17A in response to C. albicans antigens (P = 0.006). Significantly more RA patients were colonized with C. albicans in the oral cavity than healthy subjects (P = 0.02). Concomitantly, RA saliva had reduced concentrations of salivary BD2 (P = 0.02). Nonetheless, salivary fungicidal activity was preserved in RA subjects (P = 0.70).

Conclusions

RA subjects exhibit detectable impairments in oral immune responses to C. albicans, a strongly Th17-dependent opportunistic pathogen, despite an overall elevated baseline production of IL-17A.

Introduction

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by symmetric polyarthritis and systemic inflammation. Accumulating evidence implicates the cytokine interleukin (IL)-17 and CD4+ T-helper type (Th)17 cells in the pathogenesis of RA[1, 2]. IL-17 is a proinflammatory cytokine that both induces and synergizes with tumor necrosis factor (TNF) alpha to promote induction of IL-1β and IL-6 in target cells, culminating in the production of factors such as matrix metalloproteinases and reactive oxygen species that drive erosive arthritis[3]. Consistent with the role of the Th17/IL-17 axis in the pathogenesis of RA, patients with severe disease exhibit elevated frequencies of Th17 cells, and clinical responses to TNFα inhibitors in autoimmune subjects have been associated with reductions in circulating Th17 cells[4, 5].

Whereas heightened immune responses are pathogenic, RA is paradoxically associated with impaired host defense to microbes. Epidemiologic studies have consistently demonstrated a higher incidence of infection in RA patients compared with the normal population, even when the effects of medications are controlled for[6, 7]. The modern era of targeted anti-cytokine therapies has resulted in prolonged steroid-free remissions. To some extent, however, this remission has come at the cost of increased susceptibility to opportunistic pathogens, highlighting the importance of these cytokines in host defense. Antibodies against IL-17 or its receptor IL-17RA have shown promise in early clinical trials for several autoimmune conditions including RA, but their potential impact on susceptibility to infection is poorly defined[8–12].

Candida albicans is a commensal fungus that colonizes mucocutaneous surfaces including the oral cavity, tracheobronchial tree and gastrointestinal and genitourinary tracts. The Th17/IL-17A axis is essential for protective immunity to mucocutaneous candidiasis[13], and most Candida-responsive T cells are of the Th17 phenotype[14]. Humans with impaired induction of Th17 cells (for example, mutations in STAT1, STAT3 or CARD9) or defects in IL-17A signaling (for example, mutations in IL17RA or IL17F) are highly susceptible to chronic mucocutaneous candidiasis – a condition also seen in patients with circulating antibodies against Th17 cytokines, such as in autoimmune polyendocrinopathy syndrome-1 or certain thymomas[15, 16]. Somewhat surprisingly, Candida infections are not widely reported in RA[17]; however, recent epidemiologic data from patients with inflammatory bowel disease demonstrate that TNFα inhibitors increase the risk of oropharyngeal candidiasis (OPC) at rates similar to mycobacterial infections[18]. Furthermore, the emerging use of biologics targeting Th17 pathways is likely to increase the incidence of C. albicans and other fungal infections[9, 19].

Despite the known susceptibility of RA patients to infections, there is surprisingly limited information on pathogen-specific host responses in RA, especially to fungi. Furthermore, many biologics target Th17 cell generation or effector function, and yet the functional impact of RA medications on IL-17-dependent host defense is poorly understood. We therefore sought to evaluate the impact of RA on Th17 responses to C. albicans.

Methods

Subjects

RA subjects (n = 48) and healthy controls (n = 33) were recruited from the University of Pittsburgh Rheumatoid Arthritis Comparative Effectiveness Research Registry. Clinical and demographic data were then extracted from the Registry. The University of Pittsburgh institutional review board approved this study and all subjects provided written informed consent.

Peripheral blood mononuclear cell cultures

Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood using Lymphocyte Separation Media (Accurate Chemical and Scientific, Westbury, NY, USA) and buoyant density centrifugation. PMBC stimulation was performed by seeding 48-well, flat-bottom, cell culture plates with 500,000 PBMCs ± 1 × 106 heat-killed (HK) C. albicans (prepared by boiling ~4 × 108C. albicans cells for 45 minutes) or a Th17 differentiating cocktail of recombinant human IL-1β (10 ng/ml), recombinant human IL-6 (50 ng/ml), recombinant human IL-23 (20 ng/ml), recombinant human transforming growth factor beta (TGFβ; 10 ng/ml), recombinant human IL-2 (24 IU/ml), anti-IL-12 (5 μg/ml) and anti-IL-4 (5 μg/ml) (R&D Systems, Minneapolis MN, USA)[20]. Supernatants were collected after 5 days and were analyzed in triplicate for IL-17A by enzyme-linked immunosorbent assay (eBiosciences, San Diego, CA, USA). C. albicans was prepared by culturing strain CAF2-1 in yeast peptone dextrose at 30°C overnight with agitation.

Intracellular cytokine staining and flow cytometry

PBMCs were rested overnight in RPMI supplemented with 10% fetal bovine serum, l-glutamine, non-essential amino acids, sodium pyruvate, penicillin and streptomycin; 1 × 106 PBMCs were then stimulated for 4 hours with 50 ng/ml phorbol 12-myristate 13-acetate and 1 μg/ml ionomycin in the presence of Golgi Plug (BD Biosciences, Franklin Lakes, NJ, USA). Following stimulation, cells were stained with anti-CD3 Violet 450 (clone UCHT1), anti-CD4 Per CP Cy 5.5 (clone OKT4), anti-CD45RO APC-H7 (clone UCHL1), anti-CD161 PE (clone DX12), anti-CD8 FITC (clone RPA-T8), interferon gamma (IFNγ) V500 (clone B27) and anti-IL-17A APC (clone N49-653). Intracellular cytokine staining was performed with the Cytofix Cytoperm kit (BD Biosciences). Data were acquired on a BD Aria II (BD Biosciences) and were analyzed with FlowJo (Ashland, OR, USA).

Salivary assays

Saliva samples were collected by expectoration and placed in a 10× protease inhibitor cocktail (cocktail set III; Calbiochem/EMD, Gibbstown, NJ, USA), and saliva was centrifuged for 5 minutes at 550 × g. Baseline oral C. albicans carriage was determined by plating the supernatant fraction of spun saliva in triplicate on yeast peptone dextrose plates with antibiotics (to suppress growth of oral bacteria) and C. albicans colony enumeration after incubation at 30°C for 48 hours. Salivary C. albicans killing was determined by incubating the salivary supernatant at 37°C with 1 × 106C. albicans cells (strain CAF2-1) (1:1, v/v) for 1 hour[21]. C. albicans cells were plated in triplicate for colony enumeration. For β-defensin 2 (BD2) assessment, the supernatant was analyzed using a BD2 enzyme-linked immunosorbent assay kit in duplicate or triplicate as volume allowed (Phoenix Pharmaceuticals, Burlingame, CA, USA). BD2 concentrations were normalized to the total protein content of centrifuged saliva, which was measured by the bicinchoninic acid assay (BioRad, Hercules, CA, USA).

Statistical analysis

Tests for normality and variance were performed on all datasets, and two-tailed Student’s t tests or nonparametric Wilcoxon rank-sum tests were applied as indicated. Paired Student’s t tests were used to assess paired samples, and Fischer’s exact test was used to assess categorical variables. Correlations were examined using Spearman’s correlation coefficient. GraphPad Prism 4.0 was used for all statistical analyses (GraphPad, La Jolla, CA, USA).

Results

T cells from RA patients exhibit impaired Candida albicans-specific IL-17A responses

We analyzed peripheral blood samples and saliva from 48 RA subjects and 23 healthy control subjects. Demographic and clinical characteristics of the subjects are described in Table 1. All RA subjects were receiving oral disease-modifying anti-rheumatic drugs (DMARDs) and/or biologic therapy (primarily TNFα antagonists), with 17% on biologic monotherapy, 44% on oral DMARDs only and 38% receiving combination therapy. The majority of RA subjects (75%) were in remission or had low disease activity with a 28-joint Disease Activity Score <3.1.

Table 1 Patient characteristics
Full size table

To evaluate the impact of RA on specific responses to C. albicans, we co-cultured fresh PBMCs from RA subjects or healthy controls with 1 × 106 HK C. albicans for 5 days. As controls, PBMCs from the same subjects were cultured in media alone (to monitor basal IL-17A production) or with a cocktail of Th17 differentiating cytokines (IL-1β, IL-6, IL-23, anti-IL-4 and anti-IFNγ). RA subjects exhibited higher basal IL-17A production (31.5 pg/ml, range: 0 to 146.9) than healthy controls (where it was not detectable) (P = 0.02). During co-culture with Th17 differentiating cytokines, PBMCs from RA subjects and healthy controls produced similar amounts of IL-17A (mean: 159.6 pg/ml, range: 0 to 636.3 vs. mean: 166.8 pg/ml, range: 33.7 to 596.4; P = 0.91). In contrast, upon co-culture with HK C. albicans, PBMCs from RA subjects produced significantly lower IL-17A than control PBMCs (mean: 259.2 pg/ml, range: 0 to 798.9 vs. mean: 508.1 pg/ml, range: 34.8 to 925; P = 0.006) (Figure 1A). In RA subjects, levels of IL-17A from the basal PBMC cultures correlated with IL-17A levels from the Th17 differentiating cocktail cultures (r = 0.57, P = 0.003; Additional file1: Figure S1A). Therefore, despite higher basal IL-17A and a preserved capacity to respond to Th17 differentiating cytokines, CD4+ cells from RA subjects exhibited impaired C. albicans-specific Th17 responses, at least as measured in vitro.

Figure 1
figure1

Reduced Candida albicans -induced Th17/IL-17A responses in rheumatoid arthritis. Rheumatoid arthritis (RA) subjects exhibit impaired Candida albicans-specific T-cell responses compared with healthy controls. (A) Peripheral blood mononuclear cells (PBMCs) from RA subjects and controls were cultured for 5 days in media ± heat-killed (HK) C. albicans extract or T-helper type (Th)17 differentiating cytokines (interleukin (IL)-1β, IL-6, IL-23, anti-IL4 and anti-interferon gamma (anti-IFNγ)). IL-17A in cell free culture supernatants was measured by enzyme-linked immunosorbent assay (Student’s t test). (B) Tumor necrosis factor (TNF) alpha inhibitors do not exacerbate C. albicans-specific T-cell responses compared with disease-modifying anti-rheumatic drugs (DMARDs). PBMCs were cultured as in (A) with media alone, HK C. albicans extract or Th17 differentiating cytokines. IL-17A production was assessed in the indicated patient subgroups based on medication use (Mann–Whitney U test). (C) TNFα inhibitors do not alter the fraction of circulating Th17 or Th1 cells in RA subjects with controlled disease. PBMCs from RA subjects and healthy controls were gated on CD3+CD4+ lymphocytes and were analyzed for expression of IL-17A (Th17) and IFNγ+ (Th1) cells. Data were stratified by medication use (Mann–Whitney U test).

Full size image

To address the possibility that oral DMARDs and biologics caused altered C. albicans-specific responses, we stratified the analyses of IL-17A production and Th17 and Th1 frequencies by medication usage in the RA cohort. As shown in Figure 1B, there were no detectable differences in the capability of PBMCs from RA subjects treated with different classes of medications to produce IL-17A under different stimulation conditions (media alone, HK C. albicans or Th17 differentiation cocktail). Similarly, there were no significant differences in Th17 or Th1 cell frequencies in peripheral blood from RA subjects treated with oral DMARDs alone, biologics alone or combinations of oral DMARDs and biologics (Figure 1C). Biologics therefore do not exacerbate the Candida-specific impairments in DMARD-treated patients.

Rheumatoid arthritis subjects have lower proportions of Th17 cells compared with healthy controls

Th17 cells are necessary to prevent OPC, as revealed by a variety of genetic syndromes associated with chronic mucocutaneous candidiasis[15]. To determine whether the impaired C. albicans-specific response was due to a reduced frequency of total circulating Th17 cells, we compared the frequencies of IL-17A+ (Th17 cells) and IFNγ+ (Th1) CD4+ T cells in peripheral blood. RA subjects exhibited a slightly reduced percentage of Th17 cells compared with controls (0.50 ± 0.05% and 0.67 ± 0.07%, respectively; P = 0.02), and a higher percentage of Th1 cells (17.33 ± 1.5% vs. 9.85 ± 1.1%; P = 0.002) (Figure 2A).

Figure 2
figure2

Rheumatoid arthritis subjects have a lower proportion of IL-17A+but a higher proportion of IFNγ+CD4+T cells compared with controls. (A) Circulating T-helper type (Th)17 and Th1 cells. Peripheral blood mononuclear cells (PBMCs) from rheumatoid arthritis (RA) patients and healthy controls were gated on CD3+CD4+ lymphocytes and were analyzed for expression of interleukin (IL)-17A (Th17 cells) and interferon gamma (IFNγ; Th1 cells) (Mann–Whitney U test). (B) Circulating CD161+ and TEM cells. PBMCs were gated on CD3+CD4+ lymphocytes and were analyzed for expression of CD161 and CD45RO (TEM cells) (Mann–Whitney U test). (C) CD161+IL17A+ population. PBMCs were gated on CD3+CD4+CD161+ and analyzed for expression of IL-17A and IFNγ (Mann–Whitney U test). (D) TEM IL-17A+ population. PBMCs were gated on CD3+CD4+CD45RO+ lymphocytes and were analyzed for expression of IL-17A and IFNγ (Mann–Whitney U test).

Full size image

The majority of Th17 cells in healthy individuals reside in the CD161+ and effector memory (TEM, CD45RO+CCR7-) compartments, which we confirmed in this study (data not shown). We first looked at total CD161+ and TEM cells, and found no difference in the frequencies of these populations between healthy and RA subjects (Figure 2B; P = 0.82 and P = 0.36, respectively). We then looked at frequencies of T-effector subsets within these populations. Both RA subjects and healthy controls showed similar frequencies of Th17 or Th1 cells in the CD161+ compartment (respectively 1.44 ± 0.55% vs. 1.33 ± 0.11%; P = 0.11; 13.81 ± 1.48% versus 12.79 ± 1.64%; P = 0.66) (Figure 2C). There were also no differences in the relative distributions of Th17 or Th1 cells in the TEM compartment (respectively 1.21 ± 0.20% vs. 0.98 ± 0.09%; P = 0.32; 17.49 ± 0.94% vs. 19.14 ± 2.7%; P = 1). As expected, there was a significant correlation between the frequency of circulating Th17 cells and IL-17A production during PBMC co-culture with HK C. albicans (r = 0.412, P = 0.04) (Additional file1: Figure S1B). Accordingly, the impaired in vitro and in vivo pathogen-specific responses in RA subjects were associated with reductions in total circulating Th17 cells.

Rheumatoid arthritis patients exhibit reduced IL-17A-dependent anti-Candida effector responses in the oral cavity

In the oral mucosa, IL-17A induces antimicrobial proteins (AMPs) such as BD2 and salivary histatins, which are central mediators of host defense against C. albicans[22]. Sjögren’s syndrome patients and others with salivary defects are prone to OPC[23]. We previously reported that Job’s syndrome patients, who are Th17-deficient due to mutations in STAT3, exhibit enhanced oral colonization with C. albicans and concordantly reduced C. albicans killing capacity and salivary BD2 concentrations[21]. Accordingly, we sought to determine whether anti-Candida effector responses such as AMP expression in saliva were impacted in RA subjects. As shown, RA subjects were more likely to be colonized with C. albicans in the oral cavity than healthy controls (50% vs. 15%; P = 0.02) (Figure 3A). The number of C. albicans organisms detected in saliva was not statistically different between healthy and RA subjects (P = 0.53; Figure 3B), with some healthy individuals showing quite high colonization of this commensal microbe. However, RA subjects exhibited significantly lower concentrations of salivary BD2 than healthy controls (1.3 ± 0.18 vs. 2.2 ± 0.42; P = 0.02) (Figure 3C). There was no evident correlation between Candida colonization and BD2 levels (data not shown). To determine whether reduced BD2 levels were associated with a functional deficit in antifungal immunity, saliva samples were co-incubated in vitro with a constant number of cells from a reference strain of C. albicans, and survival of the fungus was assessed relative to a PBS control. As shown in Figure 3D, salivary killing activity was similar between RA subjects and healthy controls (P = 0.70). Thus, while there were increased colonization rates of Candida and reduced IL-17A-dependent salivary AMPs, salivary candidacidal function appeared to be preserved in RA subjects, which is consistent with their clinical resistance to OPC.

Figure 3
figure3

Rheumatoid arthritis subjects have impaired IL-17A-dependent anti-microbial peptide induction, but preserved oral immunity to Candida albicans . (A), (B) Candida albicans colonization in the oral cavity. Saliva (25 μl) from the indicated cohorts was analyzed for endogenous C. albicans colonization by plating on yeast peptone dextrose (YPD) agar in triplicate and performing colony enumeration. Data are presented as (A) the percent of each cohort that exhibited any oral colonization or (B) the number of colony-forming units (CFU) in each cohort (Fischer’s exact test and Mann–Whitney U test, respectively). (C) Rheumatoid arthritis (RA) subjects exhibit reduced salivary β-defensin 2 (BD2) levels. BD2 in saliva from the indicated cohorts was determined by enzyme-linked immunosorbent assay (Student’s t test). (D) RA subjects show intact salivary candidacidal activity. Salivary Candida killing was determined by centrifuging saliva to remove endogenous microbes and incubating the spun fraction with 106C. albicans (reference strain CAF2-1) for 1 hour at 37°C. Triplicate samples were plated on YPD for colony enumeration and killing is indicated as the percentage of a PBS control (Student’s t test).

Full size image

Discussion

In this study, we found that PBMCs from RA patients showed impaired Candida-induced IL-17A production, despite overall elevated basal IL-17A production and a preserved capacity of CD4+ cells to differentiate in response to Th17 differentiating cytokines in vitro. The impaired Candida-specific response was associated with an increased rate of RA subjects colonized with Candida as well as reduced expression of BD2, an IL-17A-dependent salivary AMP. Nonetheless, salivary killing activity against Candida was preserved in RA subjects. Thus, while there is clearly a trend towards increased susceptibility to C. albicans colonization in RA, much of the effector antifungal immune response is retained, consistent with the clinical resistance to oropharyngeal candidiasis in RA patients.

Genome-wide association study data point to a role for the Th17/IL-17 axis in RA, as risk alleles impact Th17 generation and maintenance (IL6R, IL2, IL21, TYK2), trafficking (CCR6) or IL-17A signal transduction (TNFAIP3)[24, 25]. Clinically, active RA has been associated with elevated fractions of Th17 cells compared with healthy controls, and individuals that respond to TNFα inhibitors are reported to show reductions in Th17 cells compared with nonresponders[4]. Erosive arthritis in most animal models is IL-17A dependent, as treatment with blocking antibodies ameliorates disease, and disease induction is mild or absent in IL-17A-deficient mice[26, 27]. Hence, agents that inhibit the Th17 pathway at multiple points, including inhibitors of JAK kinases, IL-23, IL-17A and IL-17RA, are currently being used or evaluated in RA and other autoimmune conditions[9, 28].

Because the majority of the RA patients in this study had DMARD-controlled disease, we used this group as a reference population. An acknowledged limitation is exclusion of treatment-naïve patients with poorly controlled disease. An ideal follow-up will be to assess longitudinal pathogen-specific responses, starting before drug treatment is initiated. Nonetheless, these findings are internally consistent (reduced Th17 cell frequency, reduced IL-17A-regulated AMP expression, reduced pathogen-induced IL-17A production) and recapitulate the characteristic clinical phenotype of RA, where overt susceptibility to OPC is rarely seen (preserved salivary Candida killing, minimally elevated oral Candida colonization rates). Our findings also suggest there may be a threshold effect of IL-17A in mediating host defense to Candida, where even low amounts of IL-17A are sufficient for protective immunity. Our finding that Th17 cells in RA subjects were reduced relative to controls contrasts with some prior studies, but may be explained by the fact that these patients had controlled disease with an average Disease Activity Score of 3.1 (Table 1).

RA patients generally have not been reported to show a strong susceptibility to C. albicans despite their overall increased risk for infections[17]. The reasons for this are unclear, but as only ~50% of patients with Candida esophagitis have concurrent OPC, it is possible (and supported by our data; Figure 3) that RA patients may have elevated rates of subclinical C. albicans colonization, and hence are poised to more readily progress to clinical OPC under certain circumstances; for example, targeted anti-IL-17A therapies. Additionally, innate mechanisms such as salivary killing capacity may help maintain effective immunity to C. albicans even in the face of some degree of Th17 depletion (Figure 3). It is unclear whether RA patients also exhibit impaired or altered responses to other Th17-dependent pathogens, such as Staphylococcus aureus or Klebsiella pneumonia, which would be an important line of investigation to stem from these findings.

Traditionally, candidiasis has not been linked to TNFα inhibition, but new data suggest that TNFα inhibitors may in fact increase the risk of OPC[18]. Moreover, joint pathology can be induced or exacerbated by immunization with antigens found in the cell walls of commensal fungi, such as β-1,3-glucan and zymosan; elevated C. albicans colonization rates such as we observed in Figure 3 thus have the potential to exacerbate RA symptoms[29]. The increased basal IL-17A production in this cohort coupled with impaired C. albicans-induced IL-17A induction (Figure 1A) suggests that patients with RA have elevated IL-17A production on a per-cell basis yet exhibit subclinical pathogen-specific impairments. Accordingly, selective targeting of Th17 pathways may render patients clinically susceptible to OPC or other mucocutaneous manifestations of this fungus. Susceptibility could conceivably be additionally heightened if TNFα inhibitors are used in combination with selective Th17/IL-17 inhibiting agents, since IL-17 synergizes potently with TNFα[30, 31].

Although TGFβ, IL-1β, IL-6 and IL-23 are important for the lineage commitment and/or function of human Th17 cells, data from animal models suggest that selective exposure to these cytokines during differentiation may differentially impact the function of Th17 cells. Exposure to IL-23 in T cells that were previously polarized by TGFβ and IL-6 drives pathogenic Th17 cells, whereas a lack of IL-23 results in IL-10-producing Th17 cells that restrain pathogenic Th17 cells[32, 33]. Similarly, the TGFβ3 isoform induces pathogenic Th17 cells, whereas the TGFβ1 isoform does not[34]. These data may explain the paradox in RA of having an excessively active Th17/IL-17A axis and a simultaneous susceptibility to infections. That is, patients with RA may have elevated pathogenic Th17 cells at the expense of protective Th17 cells. In this regard, the increase in Th1 cells we observed in this study (Figure 2A) suggests that Th1 cells may not compensate for a reduced IL-17 response. This would be consistent with both human and animal studies. For example, humans with hyper-IgE syndrome have impaired Th17 levels due to STAT3 mutations; these patients have normal Th1 levels but are nonetheless susceptible to mucocutaneous candidiasis. Similarly, deficient IFNγ-/- or IL-12p35-/- mice are resistant to oral and dermal candidiasis, whereas IL-23-/- mice and IL-17R-/- mice are susceptible[35, 36].

Conclusions

This study finds that patients with RA exhibit impaired C. albicans-specific IL-17A production, despite elevated basal IL-17A serum levels and a preserved capacity for Th17 cell induction in vitro. These impaired responses are associated with an increased rate of oral C. albicans colonization and reduced IL-17A-dependent AMP production in saliva. Although mucosal Candida infections are not a commonly reported side effect associated with RA, the present data suggest that biologic drugs selectively targeting the IL-23/IL-17 axis may increase the risk of RA patients to mucosal candidiasis.

Abbreviations

AMP:

antimicrobial protein

BD2:

β-defensin 2

DMARD:

disease-modifying anti-rheumatic drug

HK:

heat-killed

IFNγ:

interferon gamma

IL:

interleukin

OPC:

oropharyngeal candidiasis

PBMC:

peripheral blood mononuclear cell

RA:

rheumatoid arthritis

TGFβ:

transforming growth factor beta

Th:

T-helper type

TNF:

tumor necrosis factor.

References

  1. 1.

    McInnes IB, Schett G: Cytokines in the pathogenesis of rheumatoid arthritis. Nat Rev Immunol. 2007, 7: 429-442. 10.1038/nri2094.

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Gaffen SL: The role of interleukin-17 in the pathogenesis of rheumatoid arthritis. Curr Rheumatol Rep. 2009, 11: 365-370. 10.1007/s11926-009-0052-y.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  3. 3.

    Gaffen SL: Structure and signalling in the IL-17 receptor family. Nat Rev Immunol. 2009, 9: 556-567. 10.1038/nri2586.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  4. 4.

    Chen DY, Chen YM, Chen HH, Hsieh CW, Lin CC, Lan JL: Increasing levels of circulating Th17 cells and interleukin-17 in rheumatoid arthritis patients with an inadequate response to anti-TNF-alpha therapy. Arthritis Res Ther. 2011, 13: R126-10.1186/ar3431.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  5. 5.

    Zaba LC, Cardinale I, Gilleaudeau P, Sullivan-Whalen M, Suarez Farinas M, Fuentes-Duculan J, Novitskaya I, Khatcherian A, Bluth MJ, Lowes MA, Krueger JG: Amelioration of epidermal hyperplasia by TNF inhibition is associated with reduced Th17 responses. J Exp Med. 2007, 204: 3183-3194. 10.1084/jem.20071094.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  6. 6.

    Ni Mhuircheartaigh OM, Matteson EL, Green AB, Crowson CS: Trends in serious infections in rheumatoid arthritis. J Rheumatol. 2013, 40: 611-616. 10.3899/jrheum.121075.

    Article  PubMed  Google Scholar 

  7. 7.

    Listing J, Gerhold K, Zink A: The risk of infections associated with rheumatoid arthritis, with its comorbidity and treatment. Rheumatology (Oxford). 2013, 52: 53-61. 10.1093/rheumatology/kes305.

    CAS  Article  Google Scholar 

  8. 8.

    Hueber W, Patel DD, Dryja T, Wright AM, Koroleva I, Bruin G, Antoni C, Draelos Z, Gold MH, Psoriasis Study Group, Durez P, Tak PP, Gomez-Reino JJ, Foster CS, Kim RY, Samson CM, Falk NS, Chu DS, Callanan D, Nguyen QD, Rose K, Haider A, Di Padova F: Effects of AIN457, a fully human antibody to interleukin-17A, on psoriasis, rheumatoid arthritis, and uveitis. Sci Transl Med. 2010, 2: 52ra72-

    Article  PubMed  Google Scholar 

  9. 9.

    Patel DD, Lee DM, Kolbinger F, Antoni C: Effect of IL-17A blockade with secukinumab in autoimmune diseases. Ann Rheum Dis. 2013, 72: 116-123.

    Article  Google Scholar 

  10. 10.

    Genovese M, Van den Bosch F, Roberson S, Bojin S, Biagini I, Ryan P, Sloan-Lancaster J: LY2439821, a humanized anti-interleukin-17 monoclonal antibody, in the treatment of patients with rheumatoid arthritis. Arthritis Rheum. 2010, 62: 929-939. 10.1002/art.27334.

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Leonardi C, Matheson R, Zachariae C, Cameron G, Li L, Edson-Heredia E, Braun D, Banerjee S: Anti-interleukin-17 monoclonal antibody ixekizumab in chronic plaque psoriasis. N Engl J Med. 2012, 366: 1190-1199. 10.1056/NEJMoa1109997.

    CAS  Article  PubMed  Google Scholar 

  12. 12.

    Papp KA, Leonardi C, Menter A, Ortonne JP, Krueger JG, Kricorian G, Aras G, Li J, Russell CB, Thompson EH, Baumgartner S: Brodalumab, an anti-interleukin-17-receptor antibody for psoriasis. N Engl J Med. 2012, 366: 1181-1189. 10.1056/NEJMoa1109017.

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Vautier S, Sousa Mda G, Brown GD: C-type lectins, fungi and Th17 responses. Cytokine Growth Factor Rev. 2010, 21: 405-412. 10.1016/j.cytogfr.2010.10.001.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  14. 14.

    Acosta-Rodriguez EV, Rivino L, Geginat J, Jarrossay D, Gattorno M, Lanzavecchia A, Sallusto F, Napolitani G: Surface phenotype and antigenic specificity of human interleukin 17-producing T helper memory cells. Nat Immunol. 2007, 8: 639-646. 10.1038/ni1467.

    CAS  Article  PubMed  Google Scholar 

  15. 15.

    Huppler AR, Bishu S, Gaffen SL: Mucocutaneous candidiasis: the IL-17 pathway and implications for targeted immunotherapy. Arthritis Res Ther. 2012, 14: 217-10.1186/ar3893.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  16. 16.

    Puel A, Picard C, Cypowyj S, Lilic D, Abel L, Casanova JL: Inborn errors of mucocutaneous immunity to Candida albicans in humans: a role for IL-17 cytokines?. Curr Opin Immunol. 2010, 22: 467-474. 10.1016/j.coi.2010.06.009.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  17. 17.

    Strangfeld A, Listing J: Infection and musculoskeletal conditions: bacterial and opportunistic infections during anti-TNF therapy. Best Pract Res Clin Rheumatol. 2006, 20: 1181-1195. 10.1016/j.berh.2006.08.010.

    CAS  Article  PubMed  Google Scholar 

  18. 18.

    Ford AC, Peyrin-Biroulet L: Opportunistic infections with anti-tumor necrosis factor-alpha therapy in inflammatory bowel disease: meta-analysis of randomized controlled trials. Am J Gastroenterol. 2013, 108: 1268-1276. 10.1038/ajg.2013.138.

    CAS  Article  PubMed  Google Scholar 

  19. 19.

    Brown GD, Denning DW, Gow NA, Levitz SM, Netea MG, White TC: Hidden killers: human fungal infections. Sci Transl Med. 2012, 4: 165rv113-

    Article  Google Scholar 

  20. 20.

    Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F: Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat Immunol. 2007, 8: 942-949.

    CAS  Article  PubMed  Google Scholar 

  21. 21.

    Conti H, Baker O, Freeman A, Jang W, Li R, Holland S, Edgerton M, Gaffen S: New mechanism of oral immunity to mucosal candidiasis in hyper-IgE syndrome. Mucosal Immunol. 2011, 4: 448-455. 10.1038/mi.2011.5.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  22. 22.

    Conti HR, Gaffen SL: Host responses to Candida albicans: Th17 cells and mucosal candidiasis. Microbes Infect. 2010, 12: 518-527. 10.1016/j.micinf.2010.03.013.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  23. 23.

    Dongari-Bagtoglou A, Fidel P: The host cytokine responses and protective immunity in oropharyngeal candidiasis. J Dent Res. 2005, 84: 966-977. 10.1177/154405910508401101.

    Article  Google Scholar 

  24. 24.

    Eyre S, Bowes J, Diogo D, Lee A, Barton A, Martin P, Zhernakova A, Stahl E, Viatte S, McAllister K, Amos CI, Padyukov L, Toes RE, Huizinga TW, Wijmenga C, Trynka G, Franke L, Westra HJ, Alfredsson L, Hu X, Sandor C, de Bakker PI, Davila S, Khor CC, Heng KK, Andrews R, Edkins S, Hunt SE, Langford C, Symmons D: High-density genetic mapping identifies new susceptibility loci for rheumatoid arthritis. Nat Genet. 2012, 44: 1336-1340. 10.1038/ng.2462.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  25. 25.

    Garg A, Ahmed M, Vallejo A, Ma A, Gaffen S: The deubiquitinase A20 mediates feedback inhibition of interleukin-17 receptor signaling. Sci Signal. 2013, 6: ra44-ra55.

    Article  PubMed  PubMed Central  Google Scholar 

  26. 26.

    Cornelissen F, van Hamburg JP, Lubberts E: The IL-12/IL-23 axis and its role in Th17 cell development, pathology and plasticity in arthritis. Curr Opin Investig Drugs. 2009, 10: 452-462.

    CAS  PubMed  Google Scholar 

  27. 27.

    Iwakura Y, Ishigame H, Saijo S, Nakae S: Functional specialization of interleukin-17 family members. Immunity. 2011, 34: 149-162. 10.1016/j.immuni.2011.02.012.

    CAS  Article  PubMed  Google Scholar 

  28. 28.

    Miossec P, Kolls JK: Targeting IL-17 and TH17 cells in chronic inflammation. Nat Rev Drug Discov. 2012, 11: 763-776. 10.1038/nrd3794.

    CAS  Article  PubMed  Google Scholar 

  29. 29.

    Marijnissen RJ, Koenders MI, van de Veerdonk FL, Dulos J, Netea MG, Boots AM, Joosten LA, van den Berg WB: Exposure to Candida albicans polarizes a T-cell driven arthritis model towards Th17 responses, resulting in a more destructive arthritis. PLoS One. 2012, 7: e38889-10.1371/journal.pone.0038889.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  30. 30.

    Miossec P: Interleukin-17 in rheumatoid arthritis: if T cells were to contribute to inflammation and destruction through synergy. Arth Rheum. 2003, 48: 594-601. 10.1002/art.10816.

    CAS  Article  Google Scholar 

  31. 31.

    Shen F, Gaffen SL: Structure-function relationships in the IL-17 receptor: implications for signal transduction and therapy. Cytokine. 2008, 41: 92-104. 10.1016/j.cyto.2007.11.013.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  32. 32.

    McGeachy MJ, Bak-Jensen KS, Chen Y, Tato CM, Blumenschein W, McClanahan T, Cua DJ: TGF-beta and IL-6 drive the production of IL-17 and IL-10 by T cells and restrain T(H)-17 cell-mediated pathology. Nat Immunol. 2007, 8: 1390-1397. 10.1038/ni1539.

    CAS  Article  PubMed  Google Scholar 

  33. 33.

    Ghoreschi K, Laurence A, Yang XP, Tato CM, McGeachy MJ, Konkel JE, Ramos HL, Wei L, Davidson TS, Bouladoux N, Grainger JR, Chen Q, Kanno Y, Watford WT, Sun HW, Eberl G, Shevach EM, Belkaid Y, Cua DJ, Chen W, O'Shea JJ: Generation of pathogenic T(H)17 cells in the absence of TGF-beta signalling. Nature. 2010, 467: 967-971. 10.1038/nature09447.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  34. 34.

    Lee Y, Awasthi A, Yosef N, Quintana FJ, Xiao S, Peters A, Wu C, Kleinewietfeld M, Kunder S, Hafler DA, Sobel RA, Regev A, Kuchroo VK: Induction and molecular signature of pathogenic TH17 cells. Nat Immunol. 2012, 13: 991-999. 10.1038/ni.2416.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  35. 35.

    Conti HR, Shen F, Nayyar N, Stocum E, Sun JN, Lindemann MJ, Ho AW, Hai JH, Yu JJ, Jung JW, Filler SG, Masso-Welch P, Edgerton M, Gaffen SL: Th17 cells and IL-17 receptor signaling are essential for mucosal host defense against oral candidiasis. J Exp Med. 2009, 206: 299-311. 10.1084/jem.20081463.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  36. 36.

    Farah C, Hu Y, Riminton S, Ashman R: Distinct roles for interleukin-12p40 and tumour necrosis factor in resistance to oral candidiasis defined by gene targeting. Oral Microbiol Immunol. 2006, 21: 252-255. 10.1111/j.1399-302X.2006.00288.x.

    CAS  Article  PubMed  Google Scholar 

Download references

Acknowledgments

SB was supported by T32 DK063922. SLG was supported by the University of Pittsburgh Division of Rheumatology & Clinical Immunology and National Institutes of Health grants R01 AR054389 and R01 DE022550. MJM was supported by the University of Pittsburgh Division of Rheumatology & Clinical Immunology. Support for MCL and for data and sample acquisition was provided by National Institutes of Health grant RC2 AR058989 and Genentech, Inc. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Genentech did not have any role in the design, in the collection, analysis, and interpretation of data, in the writing of the manuscript or in the decision to submit the manuscript for publication. Genentech reviewed the manuscript prior to submission and suggested minor edits to improve its readability. The authors thank C Amity and I Metes for assistance with data collection and Dr M Simpson-Abelson for assistance with flow cytometry. The authors thank Dr Larry Moreland and the Rheumatoid Arthritis Comparative Effectiveness Research study group (Antonio Achkar MD, Rohit Aggarwal MD, Noah Bass MD, Alan Berg MD, Sherwood Chetlin MD, Daniel DeLo MD, Robyn Domsic MD, Kenneth Gold MD, Kimberly Liang MD, Douglas Lienesch MD, Kathleen McKinnon DO, Thomas Medsger MD, Niveditha Mohan MD, Chester Oddis MD, Thaddeus Osial, MD, Terence Starz MD, Bertrand Stolzer MD, Elizabeth Young MD).

Author information

Affiliations

  1. Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, 15261, USA

    Shrinivas Bishu

  2. Division of Rheumatology & Clinical Immunology, Department of Medicine, University of Pittsburgh, BST S702, 3500 Terrace Street, Pittsburgh, PA, 15261, USA

    Ee Wern Su, Erich R Wilkerson, Kelly A Reckley, Donald M Jones, Mandy J McGeachy, Sarah L Gaffen & Marc C Levesque

Authors
  1. Shrinivas Bishu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ee Wern Su
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Erich R Wilkerson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kelly A Reckley
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Donald M Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mandy J McGeachy
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sarah L Gaffen
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Marc C Levesque
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Sarah L Gaffen or Marc C Levesque.

Additional information

Competing interests

SLG has consulted for and received a research grant from Novartis. The remaining authors declare that they have no competing interests.

Authors’ contributions

SB participated in study design, performed cellular immunoassays, performed flow cytometry analysis of peripheral blood lymphocytes, performed candidacidal assays of saliva, performed data analysis and helped to draft the manuscript. EWS performed cellular staining studies and data analysis. ERW and DMJ participated in the preparation and flow cytometry analysis of peripheral blood lymphocytes from RA subjects in the study. KAR helped develop the study database and identified, recruited and collected clinical data and blood samples from RA subjects in the study. MJM participated in study design, helped perform flow cytometry analysis of peripheral blood lymphocytes, performed data analysis and helped draft manuscript. SLG and MCL conceived of the study, participated in study design, performed data analysis and helped to draft the manuscript. All authors read and approved the final manuscript.

Electronic supplementary material

Authors’ original submitted files for images

Below are the links to the authors’ original submitted files for images.

Authors’ original file for figure 1

Authors’ original file for figure 2

Authors’ original file for figure 3

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Bishu, S., Su, E.W., Wilkerson, E.R. et al. Rheumatoid arthritis patients exhibit impaired Candida albicans-specific Th17 responses. Arthritis Res Ther 16, R50 (2014). https://doi.org/10.1186/ar4480

Download citation

Keywords

  • Rheumatoid Arthritis
  • Rheumatoid Arthritis Patient
  • Th17 Cell
  • Yeast Peptone Dextrose
  • Chronic Mucocutaneous Candidiasis

Arthritis Research & Therapy

ISSN: 1478-6362

Contact us