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DNA repair in lupus
Arthritis Research & Therapy volume 16, Article number: A7 (2014)
Background
The myriad of mouse genetic alterations that result in a lupus-like phenotype and the >100 genes now known to be involved in human lupus are all probably only a hint at the potential complexity of the many mechanisms that lead to systemic lupus erythematosus (SLE).
Methods
We have applied exome sequencing to 24 SLE patients and their parents (trios) in an effort to conquer the data analysis and to identify candidate genes that may contribute when the activity of their gene products were substantially altered.
Results
The proband of one of our SLE trios had de novo mutations in RAD54B that was predicted to change ARG to GLN and to be severely damaging to protein product activity by multiple algorithms. A second much more conservative de novo mutation in DOCK8 was not predicted to be consequential. RAD54B is a component of the homologous recombination DNA repair pathway. Cells from the SLE proband were unusually sensitive to ionizing radiation by the colony survival and comet tail assays. Ionizing radiation selectively induced interferon responsive genes in cells from this patient and not from controls. Transfection of the wild-type gene into the cells from this patient led to overexpression of the RAD54B gene product and returned ionizing radiation resistance toward normal.
Conclusion
These data in addition to the other five other genes directly or indirectly involved in altering risk of SLE by influencing DNA repair (TREX1, RAD51B, XRCC1, XRCC3, and XRCC4) implicate base excision repair, nonhomologous end joining, and homologous recombination. These results suggest that multiple DNA repair mechanisms contribute to SLE susceptibility.
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Xu, J., Patel, Z., Kottyan, L. et al. DNA repair in lupus. Arthritis Res Ther 16 (Suppl 1), A7 (2014). https://doi.org/10.1186/ar4623
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DOI: https://doi.org/10.1186/ar4623
Keywords
- Systemic Lupus Erythematosus
- Systemic Lupus Erythematosus Patient
- Base Excision Repair
- Comet Tail
- Systemic Lupus Erythematosus Susceptibility