Subphenotype mapping in systemic lupus erythematosus identifies multiple novel loci associated with circulating interferon alpha
- Silvia N Kariuki1,
- Yogita Ghodke-Puranik2,
- Jessica M Dorschner2,
- Beverly S Chrabot3,
- Jennifer A Kelly4,
- Betty P Tsao5,
- Robert P Kimberly6,
- Marta E Alarcón-Riquelme4, 7,
- Chaim O Jacob8,
- Lindsey A Criswell9,
- Kathy L Sivils4,
- Carl D Langefeld10,
- John B Harley11,
- Andrew D Skol1 and
- Timothy B Niewold2Email author
© Kariuki et al.; licensee BioMed Central Ltd. 2014
Published: 18 September 2014
Systemic lupus erythematosus (SLE) is a chronic autoimmune disorder characterized by inflammation of multiple organ systems, loss of tolerance to self-antigens, and dysregulated interferon responses. SLE is both genetically and phenotypically heterogeneous, and we hypothesize that this greatly reduces the power of overall case-control studies in SLE. Increased circulating level of the cytokine interferon alpha (IFNα) is a stable, heritable trait in SLE which has been implicated in primary disease pathogenesis. Forty to 50% of patients have high IFNα, and high levels correspond with clinical differences.
To study genetic heterogeneity in SLE, we performed a case-case study comparing patients with high versus low IFNα in over 1,800 SLE cases. Four hundred European ancestry cases formed the discovery GWAS set, and 1,443 cases from a large independent multi-ancestral replication cohort were used to validate associations.
In meta-analysis, the top associations in European ancestry were PRKG1 rs7897633 (PMeta = 2.75 × 10-8) and PNP rs1049564 (PMeta = 1.24 × 10-7). We also found evidence for cross-ancestral background associations with the ANKRD44 and PLEKHF2 loci. These loci have not been previously identified in case-control SLE genetics studies. Bioinformatic analyses implicate these loci functionally in dendritic cells and natural killer cells, both of which are involved in IFNα production in SLE.
As case-control studies of complex heterogeneous diseases reach a limit of feasibility with respect to subject number and detectable effect size, the study of informative pathogenic subphenotypes becomes a highly attractive and efficient strategy for genetic discovery in complex human disease.
SNK and YG-P contributed equally to the manuscript.
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