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  • Oral presentation
  • Open Access

Failure of receptor revision in the autoreactive B cells in patients with systemic lupus erythematosus

  • 1,
  • 2,
  • 1,
  • 1 and
  • 1
Arthritis Res Ther20035 (Suppl 3) :30

https://doi.org/10.1186/ar831

  • Published:

Keywords

  • Public Health
  • Systemic Lupus Erythematosus
  • Autoimmune Disease
  • Activate Gene
  • Mature Stage

Systemic lupus erythematosus (SLE) is a prototype of autoimmune diseases characterized by B-cell hyper-reactivity and production of various autoantibodies. It has recently been found that the recombination activating gene (RAG) is re-expressed in activated mature B cells, and RAG-expressing B cells revise their surface immunoglobulin or die by failure of productive secondary rearrangement, leading to elimination of the autoreactive B cells. We have investigated the expression of RAG proteins in mature B cells in patients with SLE. Although normal mature B cells did not express RAG proteins spontaneously, activated B cells expressed RAGs. In SLE, whole mature B cells spontaneously expressed RAG proteins excessively. Because we had found that failure of receptor editing of the Vk gene with autoreactive potential (A30-Vk2) is associated with the development of anti-DNA antibody in SLE, we next focused on anti-DNA autoantibody secreting B cells. We found that anti-DNA autoantibody secreting B cells failed to re-express RAGs spontaneously, even after activation with mitogen. Thus, anti-DNA autoantibody secreting B cells did not revise their immunoglobulin gene nor die via apoptosis, leading them to develop and persist with autoantibody secretion. These findings indicate the importance of appropriate RAG re-expression for the elimination of autoreactive B cells even at the mature stage, and the absence of such events in anti-DNA secreting B cells may be important for autoantibody production in SLE.

Authors’ Affiliations

(1)
Department of Immunology and Department of Medicine, St Marianna University School of Medicine, Kawasaki, Japan
(2)
Institute of Medical Science, St Marianna University School of Medicine, Kawasaki, Japan

Copyright

© BioMed Central Ltd 2003

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