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  • Poster presentation
  • Open Access

Transcriptional co-activator CBP/p300 regulates chondrocyte-specific gene expression via association with Sox9

  • 1,
  • 1,
  • 1 and
  • 1
Arthritis Res Ther20035 (Suppl 3) :78

https://doi.org/10.1186/ar879

  • Published:

Keywords

  • Col2a1 Gene
  • Chondrocyte Differentiation
  • Cell Line SW1353
  • Joint Formation
  • Line SW1353

Chondrocytes are critical components for the precise patterning of a developing skeletal framework and articular joint formation. Sox9 is a key transcription factor that is essential for chondrocyte differentiation and chondrocyte-specific gene expressions; however, the precise transcriptional activation mechanism of Sox9 is not fully understood. Here we demonstrate that Sox9 utilizes CBP/p300 to exert its effects. Sox9 associates with CBP/p300 in chondrocyte cell line SW1353 via its carboxyl termini activation domain in a cell type-specific manner. In promoter assays, CBP/p300 enhances Col2a1, which encodes cartilage-specific type II collagen gene, the promoter activity via Sox9. Chromatin immunoprecipitation shows that p300 is bound to the Col2a1 promoter region. Furthermore, the CBP/Sox9 complex disrupter peptide suppresses Col2a1 gene expression and chondrogenesis from mesenchymal stem cells. These data demonstrate CBP/p300 functions as a coactivator of Sox9 for cartilage tissue-specific gene expression and chondrocyte differentiation.

Authors’ Affiliations

(1)
Scripps Research Institute, La Jolla, California, USA

Copyright

© BioMed Central Ltd 2003

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