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  • Open Access

Repertoire analysis of anergic B cells from patients with systemic lupus erythematosus

  • 1,
  • 1,
  • 1,
  • 1 and
  • 1
Arthritis Res Ther20035 (Suppl 3) :86

https://doi.org/10.1186/ar887

  • Published:

Keywords

  • Systemic Lupus Erythematosus
  • Gene Element
  • Lupus Patient
  • Active Systemic Lupus Erythematosus
  • Light Chain Gene

Patients with active systemic lupus erythematosus (SLE) have atypical CD19loIgD+ peripheral blood B cells that require 100-fold more anti-immunoglobulin or recombinant CD154 to induce in vitro proliferation. Additional phenotypic and in vitro functional analyses strongly suggested that the CD19loIgD+ cells are anergic. Since anergy is one of the mechanisms by which the function of autoreactive B cells can be downregulated, a comparison of the productive B-cell repertoires of CD19loIgD+ and CD19hiIgD+ subsets from the same lupus patient was undertaken to determine whether the anergic B cells were oligoclonal or otherwise enriched in cells expressing genes known to encode autoantibodies. To accomplish this, the usage of VHDJH, VkJk and Vλ Jλ gene elements by individual B cells was determined for each subset. The two subsets exhibited low mutational frequencies (CD19hiIgD+, VH = 0.6%, Vk = 0.05%, Vλ = 0.1%; CD19loIgD+, VH = 0.2%, Vk = 0.05%, Vλ = 0.2%) and involved substitutions that rarely increased the basic amino acid content. The immunoglobulin heavy and light chain repertoires of each subset were polyclonal and very similar. However, some differences from the normal repertoire were noted. For example, the Vk1 family was under-represented and Vk4 B3 and Vk5 B2 were over-represented in both subsets. Multiple small clones (average size 3.5) were present in the VH, Vk and Vλ repertoires of both subsets. A large (n = 32) clone using VH 4–34, D3-22 and JH 6 gene elements, containing FR mutations, was found. Notably, it was split two-thirds within the CD19hiIgD+ subset and one-third within the CD19loIgD+ subset. In spite of the overwhelming similarities between subsets, there were three distinct differences. The VH4 family was significantly (37% versus 17%) more abundant in the CD19loIgD+subset. Second, CD19loIgD+ Vλ Jλ clones were two-fold more numerous and more diverse in gene utilization than the CD19hiIgD+ subset. Third, B cells utilizing Vk5 B2 genes were significantly (16% versus 7%) more abundant in the CD19hiIgD+ subset. Because of the polyclonal distribution of immunoglobulin heavy and light chain genes, and the overall similarity between the two subsets, it is likely that anergy in the CD19loIgD+ subset is not antigen specific.

Authors’ Affiliations

(1)
Autoimmunity Branch, NIAMS, National Institutes of Health, Bethesda, Maryland, USA

Copyright

© BioMed Central Ltd 2003

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