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  • Open Access

Transcriptional regulation of Synoviolin is important for the proliferation of rhumatoid synovial cells

  • 1,
  • 1,
  • 1,
  • 1,
  • 2,
  • 2,
  • 1 and
  • 1
Arthritis Res Ther20035 (Suppl 3) :142

https://doi.org/10.1186/ar943

  • Published:

Keywords

  • Arthritis
  • Transcriptional Regulation
  • cDNA Library
  • Proliferation Assay
  • Proliferation Activity

We recently cloned Synoviolin, a transmembrane protein, by immunoscreening from the human cDNA library of rheumatiod synovial cells (RSCs) using anti-RSC antibodies.

Synoviolin was highly expressed in rhuematioid synovial fibroblast cells. The mice overexpressing Synoviolin developed spontaneous arthropathy. Conversely, synoviolin+/- mice were resistant to collagen-induced arthritis.

These results indicate that it is important for the prevention of arthritis to reduce the amount of Synoviolin.

We thus analyzed the transcriptional regulation of Synoviolin promoter and identified that an Ets binding site (EBS) was crucial for the transcription of Synoviolin in vitro and in vivo.

Furthermore, to investigate the effect of downregulation of Synoviolin in RSCs, we transfected the EBS decoy oligodeoxynucleotide (ODN) or Synoviolin antisense ODN into RSCs and carried out a proliferation assay using Alamar blue reagent, which resulted in the repression of proliferation activity through the suppression of Synoviolin expression. Our results suggest that the EBS decoy ODN provides a new therapeutic approach for the treatment of arthritis.

Authors’ Affiliations

(1)
Department of Genome Science and Rheumatology, Immunology and Genetics Program, Institute of Medical Science, St Marianna University School of Medicine, Kawasaki, Japan
(2)
Department of Orthopedic Surgery and Department of Dermatology and Laboratory of Molecular Medicine, Faculty of Medicine, Kagoshima University, Kagoshima, Japan

Copyright

© BioMed Central Ltd 2003

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