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  • Poster presentation
  • Open Access

Enhanced proliferative response of CD4+ T cells from patients with systemic lupus erythematosus by T-cell receptor stimulation

  • 1,
  • 2,
  • 1 and
  • 2
Arthritis Res Ther20035 (Suppl 3) :151

https://doi.org/10.1186/ar952

  • Published:

Keywords

  • Systemic Lupus Erythematosus
  • Peripheral Blood Mononuclear Cell
  • Systemic Lupus Erythematosus Patient
  • Effector Memory
  • Proliferative Capacity

Objective

To investigate the expression of the chemokine receptor CCR7, which defines distinct subsets of naive and memory T lymphocytes with different homing and effector capacities, and the proliferative capacity of CD4+ T cells by T-cell receptor (TCR) stimulation in patients with systemic lupus erythematosus (SLE)

Methods

Heparinized venous blood from SLE patients (23–53 years old; mean, 36.7 years) and age-matched and sex-matched healthy controls (HC) was collected. Peripheral blood mononuclear cells (PBMC) were freshly isolated by Ficoll-Hypaque. We stained 106 PBMC with anti-human CCR7 antibody, anti-CD45RA, anti-CD4 and anti-CD8 to characterize the phenotype of T-cell subsets. PBMC were stimulated with anti-CD3 + anti-CD28 monoclonal antibody, and cell division was analyzed by carboxyfluorescein diacetate succinimidyl ester labeling and flow cytometry in different subsets of CD4+ T cells. Data was acquired on a FACS caliber system, and analyzed using Flow-Jo software (Tree Star Inc., San Carlos, CA, USA).

Results

SLE patients (n = 27) had fewer CD45RA+CCR7+CD4+ naive T cells (32.2 ± 13.2 vs 43.5 ± 12.3, P < 0.05) and higher CD45RA-CCR7-CD4+ effector memory T cells (20.1 ± 12.2 vs 12.9 ± 5.6, P < 0.05) as compared with the HC (n = 27). No significant differences were found in the CD45RA-CCR7+ central memory CD4+ T cells and the CD8+ T-cell compartment of SLE patients as compared with HC. The appearance of cell division was more rapid in the population of CD45RA-CCR7-CD4+ T cells, and the frequency of CD45RA-CCR7-CD4+ effector memory T cells did not correlate with disease activity, disease duration, age, or treatment within the SLE group. Enhanced cell division was observed in SLE CD4+ T cells by TCR stimulation as compared with HC, but there are no significant correlations between frequency of effector CD4+ T cells and proliferative capacity within the SLE group. Furthermore, naive CD4+ T cells from SLE patients showed increased proliferative capacity compared with that of HC, but the difference was no so significant.

Conclusion

These results suggest that enhanced proliferative response of CD4+ T cells of SLE by TCR stimulation may be caused by increased distribution of the effector memory population and intrinsic defects of SLE CD4+ T cells.

Authors’ Affiliations

(1)
Division of Rheumatology, Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea
(2)
Section of Rheumatology, Department of Internal Medicine, Yale University Medical School, New Haven, Connecticutt, USA

Copyright

© The Author(s) 2003

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