Table 2 Primers used for real-time RT-PCR
Target | Sense primer | Antisense primer |
|---|---|---|
RPLP0 | GGAAACTCTGCATTCTCGCTTCCT | CCAGGACTCGTTTGTACCCGTTG |
GAPDH | CCTCCTGCACCACCAACTGCTT | GAGGGGCCATCCACAGTCTTCT |
IGF-IEa | GACATGCCCAAGACCCAGAAGGA | CGGTGGCATGTCACTCTTCACTC |
IGF-IEc | GCCCCCATCTACCAACAAGAACAC | CGGTGGCATGTCACTCTTCACTC |
HGF | TGAAATATGTGCTGGGGCTGAAA | ACAAACAAGTGGGCCACCATAATCC |
Cmet | AACCCGAATACTGCCCAGACCC | TGATATCCGGGACACCAGTTCAG |
MCP1 | GCCCTTCTGTGCCTGCTGCT | GCAGGTGACTGGGGCATTGATT |
COX1 | GGTTTGGCATGAAACCCTACACCT | CCTCCAACTCTGCTGCCATCT |
COX2 | TGGAACATGGAATTACCCAGTTTGTTG | TGTGATACTTTCTGTACTGCGGGTGG |
HSP70 | GTGGCTGGACGCCAACACCTT | TTACACACCTGCTCCAGCTCCTTC |
HSP27 | GCTGACGGTCAAGACCAAGGATG | TGAAGCACCGGGAGATGTAGCC |
αB-crystallin | GTGTTGGGAGATGTGATTGAGGTG | CTGGGATCCGGTATTTCCTGTGG |
Myogenin | CTGCAGTCCAGAGTGGGGCAGT | CTGTAGGGTCAGCCGTGAGCAG |
Myf6 | GGGCTCGTGATAACGGCTAAGGA | TGTCCACGATGGAAGAAAGGCA |
MyoD | ACGAAGGCGCCTACTACAACGA | GACACCGCCGCACTCTTCCC |
Myostatin | TGCTGTAACCTTCCCAGGACCA | GCTCATCACAGTCAAGACCAAAATCC |
Atrogin1 | TGTTACCCAAGGAAAGAGCAGTATGGA | ACGGAGCAGCTCTCTGGGTTATTG |
MuRF1 | TGGGGGAGCCACCTTCCTCT | ATGTTCTCAAAGCCCTGCTCTGTCT |
TNFα | TTCCCCAGGGACCTCTCTCTAATC | GAGGGTTTGCTACAACATGGGCTAC |
TNFRI | GGGAGGACAGCGCCCACAAG | CACGAATTCCTTCCAGCGCAAC |
TNFRII | CCACTCGGAACCAGCCACAG | CCATGGCCACCAGGGGAAGA |
IL-1β | TGCGTGTTGAAAGATGATAAGCCCA | CAAATCGCTTTTCCATCTTCTTCTTTG |
IL-1R | GGAAGGGATGACTACGTTGGGGA | CCAGCCAGCTGAAGCCTGATGTT |
IL-6 | GAGGCACTGGCAGAAAACAACC | CCTCAAACTCCAAAAGACCAGTGATG |
IL-6R | CCAGGAGGAGTTCGGGCAAG | GGGGTGGACACCTCGTTCTCA |