Fig 2

Proliferation of activated human B lymphocytes in vitro is suppressed by Acthar. a The percentage of cells that divided, b the average number of divisions of all cells (division index), and c the fold-expansion of all cells (expansion index) were assessed in CSFE-loaded human peripheral B cells cultured for 6 days under basal conditions, with added IL-4/CD40L alone, or with IL-4/CD40L plus added Acthar at ACTH analog concentrations of approximately 0.124, 1.24, or 2.49 μM. Parallel cultures contained IL-4/CD40L plus matched volumes of placebo. Cells were examined by flow cytometry and data analyzed by ANOVA (Kruskal–Wallis test, p <0.0001 for percentage of cells divided, p <0.0001 for division index, and p <0.0001 for expansion index). **Significantly different from IL-4/CD40L-stimulated samples by Dunn’s post test at p <0.01. *Significantly different from IL-4/CD40L-stimulated samples at p <0.05. Results shown are mean ± SEM for six independent experiments. Acthar H.P. Acthar Gel®, CD40L CD40 ligand, IL interleukin