Skip to main content
Download PDF
  • Research article
  • Open access
  • Published:

Immunopathologic characterization of ultrasound-defined synovitis in rheumatoid arthritis patients in clinical remission

Arthritis Research & Therapy volume 18, Article number: 74 (2016) Cite this article

Abstract

Background

Patients with rheumatoid arthritis (RA) in clinical remission may have ultrasound-defined synovitis according to the presence of power Doppler (PD) signal. The objective was to describe the immunopathologic characteristics of ultrasound-defined synovitis compared with synovitis in patients with clinically active RA.

Methods

We included between 6 and 8 ultrasound-guided synovial biopsies per patient from 20 patients with RA in clinical remission (DAS28-ESR <2.6) with PD signal, 22 synovial tissue samples (ST) from patients with clinically active RA (swollen joint with confirmed inflammatory synovial fluid) as inflammatory controls, and 10 ST from non-inflammatory controls. Immunostaining for CD3 (T lymphocytes), CD20 (B lymphocytes), CD68 (macrophages), CD117 (mast cells), hsp47 (fibroblasts), bFGF and CXCL12 (angiogenic factors) was made and quantified by digital image analysis. The number of CD31 vessels/mm2 was quantified.

Results

RA patients in remission with PD signal had significantly reduced synovial T-cell, B-cell, mast cell and fibroblast density, but similar macrophage infiltration compared with patients with clinically active RA. Vascularity, bFGF and CXCL12 were partially reduced in RA patients in remission with PD signal compared to those with active RA, but were significantly higher compared with ST from non-inflammatory controls. During the 12-month follow up, 8/20 RA patients (40 %) lost remission: all had synovial hypertrophy grade ≥2 and significantly more synovial B cells and mast cells than patients maintaining remission.

Conclusions

Asymptomatic ultrasound-defined synovitis and clinically active arthritis differ in the degree of infiltrating lymphoid, mast cells and fibroblast density, but are similar with respect to macrophage infiltration. Persistently increased angiogenic factor expression and vascularity may explain the persistence of a PD signal.

Background

Early treatment, the availability of biologic therapies and treat-to-target strategies has made remission a realistic goal in rheumatoid arthritis (RA) [1–5]. However, significant numbers of RA patients classified as being in clinical remission have detectable synovitis on ultrasound (US) or magnetic resonance imaging (MRI) [6–10]. These patients have an increased probability of reactivation of RA and/or radiological progression during follow up. The persistence of subclinical synovitis, as evaluated by power Doppler (PD) US has been associated with a high risk of flares and joint damage [11–14]. Understanding the immunopathology underlying US-defined synovitis in RA patients in remission may increase knowledge of the physiopathology of RA and identify potential biological biomarkers of further reactivation and the progression of structural damage.

Studies suggest that PD scores correlate with vascular and inflammatory cell infiltration [15, 16]. However, in RA patients in clinical remission, only retrospective, limited semiquantitative pathological data obtained from the joints of patients undergoing replacement surgery have been reported [17]. To identify the pathological basis of these findings, we aimed to analyze the immunopathologic characteristics of synovial tissue (ST) obtained from RA patients in clinical remission with US- defined synovitis (presence of PD signal) and to determine whether immunopathologic changes predict the relapse from clinical remission during a 12 months of follow up.

Methods

Patient recruitment

Consecutive patients with RA were selected, who had been in clinical remission for ≥6 months and had PD signal. Patients had a 28-joint Disease Activity Score (DAS28) <2.6 and no swollen or tender joints as evaluated by two independent rheumatologists in the RA outpatient clinic. Patients aged <18 years, with allergy to local anesthetics, anticoagulant treatment, or unhealthy skin at the site of interest, were excluded. Clinical and US examinations were performed at the outpatient clinic of the rheumatology service and the US-guided synovial biopsy was carried out within 24 h of the clinical and US examination. We obtained biopsies from 24 patients, but 4 of the biopsies were non-evaluable due to a lack of a well-defined synovial lining. Finally, 20 patients with suitable synovial samples were clinically followed for 12 months to detect relapse from remission. All 20 patients completed the follow up.

We also included ST from two control groups: (1) synovial biopsies from unselected patients with clinically active RA (n = 22), and (2) non-inflammatory ST (control) obtained on arthroscopic surgery from 10 selected patients (60 % male; mean age (± SD) 42 (±8) years), who had meniscal lesions, but had no osteoarthritic or inflammatory lesions on MRI and had histologically normal ST. The study was approved by the Ethics Committee of the Hospital Clinic of Barcelona (Comité Ético de Investigación Científica del Hospital Clínic de Barcelona, Spain (2011/6490)) and signed informed consent was obtained from each patient.

US assessment and US-guided synovial biopsy

All US assessments were performed using high-sensitivity US equipment (Acuson Antares®, Siemens AG, Erlangen, Germany), using a frequency range from 10 to 12 MHz and pulse repetition frequency between 500 and 800 Hz. Receiver gain settings were controlled to eliminate the appearance of artifacts. Joint US findings were characterized according to published Outcome Measures in Rheumatology (OMERACT) definitions [18]. An experienced sonographer (JR), blinded to the results of the clinical joint examination, evaluated both knees and 11 joints in each hand (including proximal interphalangeal joints, metacarpophalangeal joints (MCP) and wrists) for synovial hypertrophy (SH) and intra-articular PD signal according to European League Against Rheumatism (EULAR) guidelines [19]. SH and PD scores were independently quantified (grades 0–3). US-defined synovitis was based on the presence of PD signal. Intra-rater agreement was calculated as previously described [20], and was 0.81 for SH and 0.92 for PD.

US-guided synovial biopsies were performed using the same US equipment. Two operators (JDC and JR) performed all biopsies. Only joints with PD signal were selected for biopsy. Biopsies were carried out in an operating theatre and six to eight synovial biopsies were taken per procedure. Each US biopsy was made according to the technique described by Kelly et al. [21]. There were no complications after the procedures.

Histological and immunohistochemical assessments

ST were routinely fixed and embedded in paraffin. Deparaffinized sections were cooked to perform antigen retrieval when required. Slides were subsequently stained with an automated immunostainer (TechMate 500 Plus; Dako, Cambridge, UK) using the following monoclonal antibodies: anti-CD3 (clone PS1; Novocastra, Newcastle, UK) for T-lymphocytes, anti-CD20 (clone L26; Dako) for B-lymphocytes, anti-CD68 (clone KP-1; Dako) for CD68 + macrophages, anti-CD117 (rabbit anti-human polyclonal antibody; Dako) for mast cells, anti-Hsp47 monoclonal antibodies (IgG2b M16.10A1 clone; Assay Designs) for synovial fibroblasts, anti-CD31 (clone JC70A; Dako) for endothelial cells, and anti-basic fibroblast growth factor (bFGF) (polyclonal SC-79, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and anti-CXCL12 (clone K15C [22]) for angiogenic markers, which were significantly increased in serum from these patients in our previous study [20]. As a negative control, the primary antibodies were substituted by isotype-matched and concentration-matched control antibodies. The primary antibodies were subsequently detected by an avidin-biotin-peroxidase-based method (Envision System; Dako) and an aminoethylcarbazole color reaction (Sigma-Aldrich, St. Louis, MO, USA) as previously described [23]. Finally, the slides were counterstained with hematoxylin.

Digital image analysis

Stained slides were scored on digital image analysis by an independent observer (RC), who was blinded to the diagnosis and the clinical data. Only slides with well-defined lining and sub-lining areas were included. Each stained slide was scored in its entirety by dividing it into different regions. Within each region, the number of stained cells per area and the percentage of stained cells were measured in at least 20 high-power fields using the AnalySIS®Imaging processing program (Olympus®) as previously described [24].

Statistical analysis

Quantitative immunohistologic data were analyzed using the nonparametric Mann–Whitney test or the Kruskal-Wallis test with the post-hoc Dunn test where appropriate. Correlation between variables was analyzed using Spearman’s test. Values of p < 0.05 were considered significant. The statistical analyses were carried out using SPSS V.18 software.

Results

Clinical, demographic and US data

ST samples from 20 patients with RA in remission, who had a PD signal, 22 ST samples from RA patients with active synovitis (swollen and tender joint with inflammatory synovial fluid), and 10 ST samples from non-inflammatory controls were included. The clinical and demographic data for the RA patients are shown in Table 1. In both the active RA and remission RA groups, the joint with greatest disease activity as assessed by US was selected for biopsy. This means there was the highest grade of PD signal and the highest grade of SH in joints with a similar grade of PD signal. Most biopsies in the remission group were taken from the wrist and MCP, the joints that were involved more frequently. Biopsies in the active group were mostly taken from the knee due to the additional therapeutic effect of the arthroscopic lavage.

Table 1 Clinical and demographic data for patients with rheumatoid arthritis (RA)
Full size table

Patients with active disease had longer disease duration and, as expected, higher DAS28 and greater use of prednisone compared with patients in remission, who had a PD signal (Table 1). No significant differences in the percentages patients on DMARDs or biologic therapy were found between the active RA and remission RA groups. The duration of clinical remission was 37 (8–58) months (median (IQR)).

Of the 20 patients in remission, who had PD signal, 16 (80 %) also had SH grade ≥2, fulfilling a previously reported more stringent criterion of synovitis (SH grade ≥ grade 2 plus a PD signal). This definition is based on the concept that synovitis is the presence of synovial villae (hypertrophy) with active vessels (PD signal) and identifies a subgroup of RA patients in clinical remission with significantly greater disease activity and higher serum levels of angiogenic cytokines [20]. All RA patients with clinically active disease had SH grade ≥2 and a moderate-to-severe signal (PD ≥2), whereas 85 % of RA patients in clinical remission had a mild PD signal (PD = 1) and 15 % had a PD signal = 2 (Table 1).

Immunopathologic characterization of RA patients in clinical remission

We first analyzed potential differences in the density of inflammatory cell infiltration between ST from RA patients in remission, who had PD signal, and patients with clinically active RA. ST from patients in remission, who had PD signal, had significantly reduced density of CD3+ T lymphocytes (p = 0.0001), CD20+ B lymphocytes (p = 0.0001) and CD117+ mast cells (p = 0.0002) compared with patients with clinically active RA. No significant differences in the density of total, lining, or sub-lining CD68+ macrophages were found between patients with clinically active RA and those in remission (Fig. 1).

Fig. 1
figure 1

Immunohistochemical analysis of inflammatory infiltrating cell populations in synovial tissues of rheumatoid arthritis (RA) patients in remission, who had power Doppler signal, compared to patients with clinically active RA. The density of CD68 (macrophages) in the synovial lining (L) and sub-lining (SL), CD3 (T lymphocytes), CD20 (B lymphocytes) and CD117 (mast cells), as assessed by quantitative digital image analysis is shown. Data are represented as mean ± SD. NS not significant

Full size image

As vascular and stromal changes have been suggested to explain the persistence of subclinical inflammation, we analyzed these changes in ST from the three study groups. Patients in remission, who had a PD signal, had significantly reduced density of CD31+ blood vessels compared with patients with active RA (p = 0.04), but had significantly increased density compared with non-inflammatory ST samples from controls (p = 0.02). The density of hsp47+ fibroblastic cells was significantly reduced in RA patients in remission who had a PD signal compared to patients with active RA (p = 0.002) and was similar to that in controls with no inflammatory disease (Fig. 2).

Fig. 2
figure 2

Immunohistochemical analysis of stromal and vascular cells, and expression of angiogenic factors in synovial tissues (ST) from rheumatoid arthritis (RA) patients and non-inflammatory synovial tissues from controls (Normal ST). The density of of hsp47 (fibroblasts), CD31 (blood vessels), and the expression of basic fibroblast growth factor (bFGF) and CXCL12, as assessed by quantitative digital image analysis is shown. Data are represented as mean ± SD. NS not significant

Full size image

We also analyzed the expression of bFGF and CXC12, two angiogenic factors that we previously found to be increased in serum from RA patients in remission, who had PD signal, [20]. The expression of CXCL12 was significantly increased in patients with clinically active RA compared with those in remission, but CXCL12 expression in the remission group was still significantly higher than in the control group who did not have inflammatory disease (Fig. 2). In contrast, the expression of bFGF in the remission group did not significantly differ from that in the group clinically active RA, but was significantly higher than in the non-inflammatory control group (Fig. 2).

High risk of flare in patients with RA in remission who had power Doppler signal and synovial hypertrophy ≥2

All patients in remission, who had PD signal, were followed for 12 months, during which 8 (40 %) came out of remission, all of whom met a more stringent criterion for US synovitis (PD signal plus SH grade ≥2) on biopsy. These patients had a significantly higher density of CD20+ B cells (p = 0.009), CD117+ mast cells (p = 0.010), and a non-significant trend towards higher density of lining macrophages (p = 0.079) than patients maintaining clinical remission (Table 2).

Table 2 Immunopathologic features of patients with rheumatoid arthritis who had come out of remission at 12 months
Full size table

Discussion

To our knowledge, this is the first study to quantitatively analyze changes in the infiltrating and resident cell components that characterize RA patients in remission with US-defined synovitis, based on the presence of PD signal. Previous studies in active or end-stage joints in patients with RA and osteoarthritis have shown correlation between US findings, mainly PD scores, and vascularity [15]. In a recent study of patients with clinically active RA there was significant correlation between inflammatory cell infiltration (CD68 macrophages and CD3 T lymphocytes) and vascularity with a PD color fraction [16].

Our results show that RA patients in clinical remission, who have a persistent PD signal, have high macrophage infiltration, comparable to patients with clinically active RA, and increased vascularity. In contrast, lymphocytic and mast cell infiltration and fibroblastic hyperplasia were significantly reduced in these patients compared with patients with clinically active disease. The comparison with ST from individuals without inflammatory disease reinforces these conclusions as there was increased vascularity but similar fibroblastic density in RA patients in remission compared with synovium from patients without inflammatory disease. Furthermore, the expression of angiogenic factors, particularly bFGF, which was found to be the best systemic biomarker of US synovitis in our previous study [20], was also significantly elevated in the ST of these patients.

These results have implications for the mechanistic understanding of US-defined synovitis in RA patients in clinical remission. Synovial macrophage infiltration is the best marker of active disease, and the most sensitive to change after effective therapy [25, 26]. Therefore, the finding of persistent macrophage infiltration suggests that US synovitis does not differ physiopathologically from clinically active synovitis. Despite the absence of clinical signs (i.e., non-tender and non-swollen joints), RA patients in remission, who have PD signal, would appear to have a pathologic status whereby macrophage depletion has not been achieved by therapy.

Our results also confirm the link between PD and increased vascularity in these patients, as was previously suggested to be characteristic of joints in clinically active disease [15, 16]. The persistent expression of angiogenic bFGF and macrophage infiltration provides a mechanistic explanation for this pathologic observation. Interestingly, bFGF-induced angiogenesis has been linked to macrophage infiltration, underlining the pathogenic role of this factor in RA [27, 28].

The main pathologic difference between clinical and US-defined synovitis was in lymphocytes, mast cells and fibroblasts, which were significantly-reduced in ST from RA patients in remission who had PD signal. These cells might, therefore, play a relevant role in the clinical expression of synovitis, but identification of the potential mechanisms remains speculative. It is also interesting that the levels of mast cell and B lymphocyte infiltration were associated with clinical progression to symptomatic, clinically active RA during the follow up.

Mast cells play an essential role in some animal models of arthritis [29] and are an important synovial reservoir of pro-inflammatory cytokines, including TNFα and IL-17, in RA [30, 31]. However, few studies have involved analysis of their changes or their prognostic implications in patients on treatment [32]. Despite the significant reduction in asymptomatic US synovitis compared with clinical synovitis, the levels in patients in remission correlated with further clinical reactivation during follow up, supporting a role for mast-cells in disease activity and in the transition from subclinical to clinically active synovitis.

A higher density of synovial B lymphocytes was also associated with RA reactivation. The efficacy of anti-CD20 therapy in RA has shown the pathogenic relevance of B cells, but the specific role of local synovial B-cells is less clear [33]. An increased basal density of B cells has been associated with a worse therapeutic response to anti-CD20 but not to other therapies [34, 35].

Our study was limited by the relatively small sample size. However, we were able to take synovial biopsies from >80 % of eligible patients (meeting the PD signal criteria). However, ST from the group with clinically active RA was taken from joints with clinically active disease (swollen joints with inflammatory synovial fluid) and was included retrospectively to specifically compare their immunopathologic characteristics with the RA remission group. Therefore, the association between the immunopathologic findings and disease reactivation are only exploratory and require further confirmation. Finally, 80 % of patients with RA in remission, who had PD signal, also had synovial hypertrophy grade ≥2, thus, meeting a more stringent criterion for US synovitis [20]. These patients had a high risk of coming out of remission, as 50 % had arthritic flares during follow up.

Conclusions

This prospective study shows that the presence of US-defined synovitis based on the presence of PD signal in RA patients in clinical remission has a histopathological correlate, which is characterized by an unbalanced reduction in the cellular components of clinically active RA synovitis, with persistent macrophage and vascular components. These results, together with our previous observations on serum biomarkers [20], support a specific cytokine profile in these patients, with the angiogenic factor bFGF emerging as a potentially relevant mediator.

Abbreviations

CXCL:

CC-chemokine ligand

DAS28:

28-joint Disease Activity Score

ESR:

erythrocyte sedimentation rate

bFGF:

basic fibroblast growth factor

hsp:

heat shock protein

IL:

interleukin

IQR:

interquartile range

MCP:

metacarpophalangeal joints

MRI:

magnetic resonance imaging

PD:

power Doppler

RA:

rheumatoid arthritis

SD:

standard deviation

SH:

synovial hypertrophy

ST:

synovial tissue

TNF-α:

tumor necrosis factor-α

US:

ultrasound

References

  1. Goekoop-Ruiterman YP, de Vries-Bouwstra JK, Allaart CF, van Zeben D, Kerstens PJ, Hazes JM, et al. Clinical and radiographic outcomes of four different treatment strategies in patients with early rheumatoid arthritis (the BeSt study): a randomized, controlled trial. Arthritis Rheum. 2005;52:3381–90.

    Article  CAS  PubMed  Google Scholar 

  2. Mottonen T, Hannonen P, Leirisalo-Repo M, Nissila M, Kautiainen H, Korpela M, et al. Comparison of combination therapy with single-drug therapy in early rheumatoid arthritis: a randomised trial. FIN-RACo trial group. Lancet. 1999;353:1568–73.

    Article  CAS  PubMed  Google Scholar 

  3. Grigor C, Capell H, Stirling A, McMahon AD, Lock P, Vallance R, et al. Effect of a treatment strategy of tight control for rheumatoid arthritis (the TICORA study): a single-blind randomised controlled trial. Lancet. 2004;364:263–9.

    Article  PubMed  Google Scholar 

  4. Breedveld FC, Weisman MH, Kavanaugh AF, Cohen SB, Pavelka K, van Vollenhoven R, et al. The PREMIER study: A multicenter, randomized, double-blind clinical trial of combination therapy with adalimumab plus methotrexate versus methotrexate alone or adalimumab alone in patients with early, aggressive rheumatoid arthritis who had not previous methotrexate treatment. Arthritis Rheum. 2006;54:26–37.

    Article  CAS  PubMed  Google Scholar 

  5. Cañete JD, Pablos JL. Biologic therapy in rheumatoid arthritis. Curr Top Med Chem. 2013;13:752–9.

    Article  PubMed  Google Scholar 

  6. Brown AK, Quinn MA, Karim Z, Conaghan PG, Peterfy CG, Hensor E, et al. Presence of significant synovitis in rheumatoid arthritis patients with disease-modifying antirheumatic drug-induced clinical remission: evidence from an imaging study may explain structural progression. Arthritis Rheum. 2006;54:3761–73.

    Article  CAS  PubMed  Google Scholar 

  7. Saleem B, Brown AK, Keen H, Nizam S, Freeston J, Karim Z, et al. Disease remission state in patients treated with the combination of tumor necrosis factor blockade and methotrexate or with disease-modifying antirheumatic drugs: a clinical and imaging comparative study. Arthritis Rheum. 2009;60:1915–22.

    Article  CAS  PubMed  Google Scholar 

  8. Wakefield RJ, Freeston JE, Hensor EM, Bryer D, Quinn MA, Emery P. Delay in imaging versus clinical response: a rationale for prolonged treatment with anti-tumor necrosis factor medication in early rheumatoid arthritis. Arthritis Rheum. 2007;57:1564–7.

    Article  PubMed  Google Scholar 

  9. Scire CA, Montecucco C, Codullo V, Epis O, Todoerti M, Caporali R. Ultrasonographic evaluation of joint involvement in early rheumatoid arthritis in clinical remission: power Doppler signal predicts short-term relapse. Rheumatology (Oxford). 2009;48:1092–7.

    Article  CAS  Google Scholar 

  10. Peluso G, Michelutti A, Bosello S, Gremese E, Tolusso B, Ferraccioli G. Clinical and ultrasonographic remission determines different chances of relapse in early and long standing rheumatoid arthritis. Ann Rheum Dis. 2011;70:172–5.

    Article  PubMed  Google Scholar 

  11. Kawashiri SY, Suzuki T, Nakashima Y, Horai Y, Okada A, Iwamoto N, et al. Ultrasonographic examination of rheumatoid arthritis patients who are free of physical synovitis: power Doppler subclinical synovitis is associated with bone erosion. Rheumatology (Oxford). 2014;53:562–9.

    Article  Google Scholar 

  12. Nguyen H, Ruyssen-Witrand A, Gandjbakhch F, Constantin A, Foltz V, Cantagrel A. Prevalence of ultrasound-detected residual synovitis and risk of relapse and structural progression in rheumatoid arthritis patients in clinical remission: a systematic review and meta-analysis. Rheumatology (Oxford). 2014;53:2110–8.

    Article  Google Scholar 

  13. Foltz V, Gandjbakhch F, Etchepare F, Rosenberg C, Tanguy ML, Rozenberg S, et al. Power Doppler ultrasound, but not low-field magnetic resonance imaging, predicts relapse and radiographic disease progression in rheumatoid arthritis patients with low levels of disease activity. Arthritis Rheum. 2012;64:67–76.

    Article  PubMed  Google Scholar 

  14. Brown AK, Conaghan PG, Karim Z, Quinn MA, Ikeda K, Peterfy CG, et al. An explanation for the apparent dissociation between clinical remission and continued structural deterioration in rheumatoid arthritis. Arthritis Rheum. 2008;58:2958–67.

    Article  CAS  PubMed  Google Scholar 

  15. Walther M, Harms H, Krenn V, Radke S, Faehndrich TP, Gohlke F. Correlation of power Doppler sonography with vascularity of the synovial tissue of the knee joint in patients with osteoarthritis and rheumatoid arthritis. Arthritis Rheum. 2001;44:331–8.

    Article  CAS  PubMed  Google Scholar 

  16. Andersen M, Ellegaard K, Hebsgaard JB, Christensen R, Torp-Pedersen S, Kvist PH, et al. Ultrasound colour Doppler is associated with synovial pathology in biopsies from hand joints in rheumatoid arthritis patients: a cross-sectional study. Ann Rheum Dis. 2013;73:678–83.

    Article  PubMed  Google Scholar 

  17. Anandarajah A, Thiele R, Giampoli E, Monu J, Seo GS, Feng C, et al. Patients with rheumatoid arthritis in clinical remission manifest persistent joint inflammation on histology and imaging studies. J Rheumatol. 2014;41:2153–60.

    Article  PubMed  Google Scholar 

  18. Wakefield RJ, Balint PV, Szkudlarek M, Filippucci E, Backhaus M, D’Agostino MA, et al. Musculoskeletal ultrasound including definitions for ultrasonographic pathology. J Rheumatol. 2005;32:2485–7.

    PubMed  Google Scholar 

  19. Backhaus M, Burmester GR, Gerber T, Grassi W, Machold KP, Swen WA, et al. Guidelines for musculoskeletal ultrasound in rheumatology. Ann Rheum Dis. 2001;60:641–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Ramirez J, Ruiz-Esquide V, Pomes I, Celis R, Cuervo A, Hernandez MV, et al. Patients with rheumatoid arthritis in clinical remission and ultrasound-defined active synovitis exhibit higher disease activity and increased serum levels of angiogenic biomarkers. Arthritis Res Ther. 2014;16:R5.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Kelly S, Humby F, Filer A, Ng N, Di Cicco M, Hands RE, et al. Ultrasound-guided synovial biopsy: a safe, well-tolerated and reliable technique for obtaining high-quality synovial tissue from both large and small joints in early arthritis patients. Ann Rheum Dis. 2013;74:611–7.

    Article  PubMed  Google Scholar 

  22. Pablos JL, Amara A, Bouloc A, Santiago B, Caruz A, Galindo M, et al. Stromal-cell derived factor is expressed by dendritic cells and endothelium in human skin. Am J Pathol. 1999;155:1577–86.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Cañete JD, Celis R, Moll C, Izquierdo E, Marsal S, Sanmarti R, et al. Clinical significance of synovial lymphoid neogenesis and its reversal after anti-tumour necrosis factor alpha therapy in rheumatoid arthritis. Ann Rheum Dis. 2009;68:751–6.

    Article  PubMed  Google Scholar 

  24. Haringman JJ, Vinkenoog M, Gerlag DM, Smeets TJ, Zwinderman AH, Tak PP. Reliability of computerized image analysis for the evaluation of serial synovial biopsies in randomized controlled trials in rheumatoid arthritis. Arthritis Res Ther. 2005;7:R862–7.

    Article  PubMed  PubMed Central  Google Scholar 

  25. Haringman JJ, Gerlag DM, Zwinderman AH, Smeets TJ, Kraan MC, Baeten D, et al. Synovial tissue macrophages: a sensitive biomarker for response to treatment in patients with rheumatoid arthritis. Ann Rheum Dis. 2005;64:834–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Bresnihan B, Pontifex E, Thurlings RM, Vinkenoog M, El-Gabalawy H, Fearon U, et al. Synovial tissue sublining CD68 expression is a biomarker of therapeutic response in rheumatoid arthritis clinical trials: consistency across centers. J Rheumatol. 2009;36:1800–2.

    Article  PubMed  Google Scholar 

  27. Tigges U, Hyer EG, Scharf J, Stallcup WB. FGF2-dependent neovascularization of subcutaneous Matrigel plugs is initiated by bone marrow-derived pericytes and macrophages. Development. 2008;135:523–32.

    Article  CAS  PubMed  Google Scholar 

  28. Andrés G, Leali D, Mitola S, Coltrini D, Camozzi M, Corsini M, et al. A pro-inflammatory signature mediates FGF2-induced angiogenesis. J Cell Mol Med. 2009;13:2083–108.

    Article  PubMed  Google Scholar 

  29. Lee DM, Friend DS, Gurish MF, Benoist C, Mathis D, Brenner MB. A cellular link between autoantibodies and inflammatory arthritis. Science. 2002;297:1689–92.

    Article  CAS  PubMed  Google Scholar 

  30. Abraham SN, Arock M. Mast cells and basophils in innate immunity. Semin Immunol. 1998;10:373–81.

    Article  CAS  PubMed  Google Scholar 

  31. Hueber AJ, Asquith DL, Miller AM, Reilly J, Kerr S, Leipe J, et al. Mast cells express IL17A in rheumatoid arthritis synovium. J Immunol. 2010;184:3336–40.

    Article  CAS  PubMed  Google Scholar 

  32. Noordenbos T, Yeremenko N, Gofita I, van de Sande M, Tak PP, Cañete JD, et al. Interleukin-17-positive mast cells contribute to synovial inflammation in spondylarthritis. Arthritis Rheum. 2012;64:99–109.

    Article  CAS  PubMed  Google Scholar 

  33. Bugatti S, Manzo A, Bombardieri M, Vitolo B, Humby F, Kelly S, et al. Synovial tissue heterogeneity and peripheral blood biomarkers. Curr Rheumatol Rep. 2011;13:440–8.

    Article  CAS  PubMed  Google Scholar 

  34. Teng YK, Levarht EW, Toes RE, Huizinga TW, van Laar JM. Residual inflammation after rituximab treatment is associated with sustained synovial plasma cell infiltration and enhanced B cell repopulation. Ann Rheum Dis. 2009;68:1011–6.

    Article  CAS  PubMed  Google Scholar 

  35. Wijbrandts CA, Dijkgraaf MG, Kraan MC, Vinkenoog M, Smeets TJ, Dinant H, et al. The clinical response to infliximab in rheumatoid arthritis is in part dependent on pretreatment tumour necrosis factor alpha expression in the synovium. Ann Rheum Dis. 2008;67:1139–44.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

The authors thank Dr. Beatriz Bravo (Servicio de Traumatología y Cirugía Ortopédica, Hospital 12 de Octubre, Madrid, Spain) for providing non-inflammatory ST. All the data from this study are included in the manuscript. We have no more data to share. This study was supported by grants PI11/1890 (JDC), PI I12/439 (JLP), Rio Hortega Program (RF), and RIER RD12/09, from the Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Spain (co-financed by FEDER, European Union “Una manera de hacer Europa”).

Author information

Authors and Affiliations

  1. Arthritis Unit, Rheumatology Department, Hospital Clinic of Barcelona and IDIBAPS, c/ Villarroel, 170, 08036, Barcelona, Spain

    Julio Ramírez, Raquel Celis, Virginia Ruiz-Esquide, Andrea Cuervo, Raimon Sanmartí & Juan D. Cañete

  2. Rheumatology Department, Instituto de Investigación Hospital 12 de Octubre (I + 12), Avda, Córdoba, s/n, 28041, Madrid, Spain

    Alicia Usategui, Regina Faré & José L. Pablos

Authors
  1. Julio Ramírez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Raquel Celis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alicia Usategui
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Virginia Ruiz-Esquide
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Regina Faré
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Andrea Cuervo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Raimon Sanmartí
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. José L. Pablos
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Juan D. Cañete
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Juan D. Cañete.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

JR helped design the study, performed data acquisition and data analysis, and helped write the paper. RC, AU, and RF performed immunohistochemical analysis, data acquisition and data analysis, and drafted the manuscript. VR-E and AC contributed to data acquisition and analysis and revised the manuscript. RS helped design the study and interpret the data, and helped to draft the manuscript. JDC conceived the study, and JDC and JLP designed the study, participated in the research and data analysis, and wrote the paper. All authors read and approved the final manuscript.

José L. Pablos and Juan D. Cañete shared senior authorship.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ramírez, J., Celis, R., Usategui, A. et al. Immunopathologic characterization of ultrasound-defined synovitis in rheumatoid arthritis patients in clinical remission. Arthritis Res Ther 18, 74 (2016). https://doi.org/10.1186/s13075-016-0970-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s13075-016-0970-9

Keywords

Arthritis Research & Therapy

ISSN: 1478-6362

Contact us