Fig. 4
From: The roles of neutrophil serine proteinases in idiopathic inflammatory myopathies
Serum NSPs degraded VE-cadherin, disrupted tube formation, and increased permeability of HDMECs. After stimulating HDMECs with 20% serum from patients for 24 h, expression of VE-cadherin (110 kDa) decreased. VE-Cadherin expression could be neutralized by nonspecific inhibitor PMSF (a, b). After treating HDMECs with 20% serum from patients, tube formation ability of HDMECs decreased. This function of serum could be alleviated by PMSF (c, d). After treating HDMECs with 20% serum from patients, permeability of HDMECs to human PBMCs was significantly increased. This increase could be lessened by PMSF (e). Values are mean ± SEM. DM dermatomyositis, NC normal control, PM polymyositis, PMSF phenylmethylsulphonyl fluoride