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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Impairment of glyoxalase-1, an advanced glycation end-product detoxifying enzyme, induced by inflammation in age-related osteoarthritis

Fig. 5

Involvement of nitric oxide (NO) in the downregulation of glyoxalase-1 (Glo-1) induced by interleukin-1β (IL-1β) in a primary culture of murine chondrocytes. Cells were treated with an inhibitor of NO synthase, l-NAME (5 mM). a Quantitative RT-PCR analysis of mRNA levels of Glo-1 relative to hypoxanthine guanine phosphoribosyltransferase (HPRT), n = 5 for each condition. b Dimeric Glo-1 protein expression relative to Ponceau staining, n = 5 in each group. c Glo-1 enzymatic activity, n = 6 in each group. d Ponceau staining and Western blot as an illustrative example. Data are presented as median and interquartile range. **p = 0.0313, ***p = 0.0156

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Arthritis Research & Therapy

ISSN: 1478-6362

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