Fig. 5
From: Rheumatoid arthritis sera antibodies to citrullinated collagen type II bind to joint cartilage
Private sera showed specific binding to cartilage in vitro. A Private sera reactivity to arthritic DBA1/J joint obtained at D90 after 1st immunization (arthritis score > 12) tissue. The x-axis shows the citrulline sites of sera responded peptides. B IHC results of sera that had private response to 12-cit cyclic COL2 peptide. C IHC results of sera that had private response to 19-cit cyclic COL2 peptide (specific to C1 epitope). The sera did not bind to arthritic joint tissue listed: (a) obtained at D90 after immunization from C1 mutated mouse (historic max arthritis score = 14) and (b) arthritic DBA joint tissues as described previously but blocked with 5 mg/ml CB20 before sera incubation