Two B cell populations differentially expressing IgVH mRNAs in human RA synovium
© The Author(s) 2004
Received: 16 January 2004
Published: 24 February 2004
The lymphocytic infiltrates sometimes organized in ectopic germinal centres in rheumatoid arthritis (RA) synovium contain locally activated B cells expressing hypermutated immunoglobulin transcripts and recombination-activating genes (Rag), suggesting an ongoing antigen driven process directly in synovium.
To test this hypothesis, mutational frequencies of immunoglobulin mRNAs, signs of isotype switching and Rag gene expression in individual RA synovial B cells were analyzed.
Single-cell RT-PCR was used to analyze individual synovial CD19+CD38+ and CD19+IgM+ B cells (as the reference population) from two RA patients.
Mutational frequencies of IgVH mRNAs in RA synovial CD19+CD38+ and CD19+IgM+ populations
Total of nt