Assessment of histological alterations in cartilage and extracellular matrix driven by collagen-induced arthritis in Macaca fascicularis
© Amizuka et al.; licensee BioMed Central Ltd. 2012
Published: 29 February 2012
Arthritis is characterized by progressive cartilage erosion, inflammation of adjoining soft tissues and collapse of subchondral bone due to enhanced osteoclastic resorption. Human joints are complex structures formed by synovial tissues, articular cartilage and subchondral bone tissue. Believing on the similarities of normal joints in humans and monkeys, we have employed a model of collagen-induced arthritis in Macaca fascicularis (or crab-eating monkey) in an attempt to evaluate the histological alterations caused by such condition in the extracellular matrix of the articular cartilage.
Materials and methods
Intermediate phalangeal proximal joints of six Macaca fascicularis suffering from collagen-induced arthritis were extracted and fixed with 4% paraformaldehyde solution. Samples were also taken from disease-free animals as controls. Tissues were embedded in paraffin or epoxy resin for histochemical and ultrastructural observations. Paraffin sections were used for alkaline phosphatase (ALP), tartrate-resistant acid phosphatase (TRAP), cathepsin K, MMP-1, type II collagen, CTX-II (fragmented type II collagen) and fibronectin staining assessments.
Based on the evidence provided, it is possible that matrix degradation starts not from the adjacent subchondral bone, but from the most superficial region of the arthritic cartilage.
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