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Epigenetic contributions in the development of rheumatoid arthritis


Rheumatoid arthritis (RA) is an autoimmune disease, characterized by chronic inflammation of the joints with severe pain and swelling, joint damage and disability, which leads to joint destruction and loss of function. Despite extensive research efforts, the underlying cause for RA is still unknown and current therapies are more or less effective in controlling symptoms but still fail to cure the disease. In recent years, epigenetic modifications were found to strongly contribute to the development of RA by affecting diverse aspects of the disease and modifying gene expression levels and behavior of several cell types, first and foremost joint resident synovial fibroblasts (SF). RASF are the most common cell type at the site of invasion. Owing to their aggressive, intrinsically activated phenotype, RASF are active contributors in joint damage. RASF are characterized by their ability to secrete cytokines, chemokines and joint-damaging enzymes. Furthermore, these cells are resistant to apoptosis, leading to hyperplasia of the synovium. In addition, RASF have invasive and migratory properties that could lead to spreading of the disease to unaffected joints. Epigenetic modifications, including DNA methylation and post-translational histone modifications, such as histone (de)acetylation, histone methylation and histone sumoylation were identified as regulatory mechanisms in controlling aggressive cell activation in vitro and in disease outcome in animal models in vivo. In the last 5 years, the field of epigenetics in RA has impressively increased. In this review we consider the role of diverse epigenetic modifications in the development of RA, with a special focus on epigenetic modifications in RASF.

Rheumatoid arthritis (RA) is a chronic inflammatory disease affecting about 1% of the population. Both genetic predisposition and environmental factors, as well as an aberrant activation of the innate and adapted immune system, play a role in the pathogenesis. The major pathological phenomena of RA are the massively elevated levels of inflammatory cytokines produced by activated B cells and T cells, as well as other cell populations and a synovial hyperplasia, resulting in damage of the cartilage and bone. Characteristically, peripheral joints are often affected in a symmetric distribution. During active disease states, RA leads to swelling and pain, resulting in disability, joint destruction and, ultimately, loss of function. A number of characteristics - such as the occurrence of erosions at the time of presentation shortly after onset of symptoms, synovial infiltrates in clinically unaffected joints and the appearance of autoantibodies long before the onset of the disease - suggest that the disease develops long before clinical symptoms become apparent [1]. At least two subsets of RA exist, which are divided by the presence or absence of antibodies against citrullinated peptide antigens (ACPA). The presence or absence of ACPA has been proven one of the best clinical predictors for disease outcome. Recent genome-wide association studies suggested that ACPA-positive RA and ACPA-negative RA also differ significantly in the frequency of risk alleles. The most important genetic risk factors accounting for 30 to 50% of the overall risk for RA are mainly confined to the human leukocyte antigen locus [2].

In recent years, knowledge about pathogenic mechanisms and treatment strategies in RA has significantly improved. Only one-third of patients respond well to the first-line treatment methotrexate [3], however, and although the application of biological drugs including therapeutic antibodies directed against TNFα is of great benefit for affected patients, all currently available therapies are more or less effective in controlling symptoms and there is still a subgroup of nonresponding patients. No cure for the disease so far exists and the entire cause of the disease remains elusive.

In the last years, resident synovial fibroblasts (SF) emerged as key players in the development of RA that actively contribute to joint destruction. RASF are the most common cell type at sites of invasion. By implanting healthy human cartilage together with RASF into severe combined immunodeficient mice, Lefèvre and colleagues demonstrated that activated RASF migrate in vivo and spread the disease to sites of implanted cartilage. Whereas RASF actively degraded human cartilage, control implanted SF from osteoarthritis (OA) patients and dermal fibroblasts from healthy donors did not. Since RASF were able to destroy cartilage even in the absence of cellular and humoral immune responses, this study revealed that RASF are capable of maintaining their activated phenotype without further stimulation of the inflammatory environment in the synovium [4]. RASF differ from healthy synovial fibroblasts by their morphology and an aberrant gene expression pattern [5]. RASF are characterized by the expression of antiapoptotic molecules, protooncogenes and a lack of expression of tumor suppressor genes. Owing to their ability to produce pro-inflammatory cytokines and chemokines, RASF further attract inflammatory cells of the immune system to the synovium. In addition, RASF produce enzymes such as matrix metalloproteinases (MMPs) that promote invasion into and destruction of cartilage [5]. In recent years RASF were also characterized by aberrant epigenetic modifications that might explain their intrinsic activated phenotype (Figure 1), and in the future they might provide the missing link between RA, risk factors and a lack of therapy response.

Figure 1
figure 1

Epigenetic modifications in rheumatoid arthritis synovial fibroblasts contribute to their aggressive phenotype. Rheumatoid arthritis synovial fibroblasts (RASF) are characterized by excessive activation and an apoptosis-resistant phenotype, leading to hyperplasia of the synovium. Furthermore, RASF are capable of producing chemokines and cytokines, and thereby promote inflammation. The production of matrix metalloproteinases (MMPs) leads to invasion of RASF into cartilage. These characteristics of RASF contribute to the hallmarks of RA, namely joint destruction, loss of function, disability and pain. Changes in epigenetic modifications, such as DNA methylation, histone acetylation, histone methylation, and histone sumoylation, were described in pathologically activated RASF.

Originally, epigenetic modifications were described as heritable alterations of gene expression or cellular phenotypes that were caused by mechanisms other than changes in the DNA sequence. In a more revised definition of epigenetics, also environmentally induced and shorter lived, reversible alterations of the histone code were included [6]. Epigenetic modifications determine the stability of the chromatin structure, the genome integrity, the modulation of gene expression, embryonic development, genomic imprinting and X-chromosome inactivation (XCI) in females [7]. Beside DNA methylation, large networks of post-translational modifications on histone tails, such as acetylation, phosphorylation, methylation, ubiquitination or sumoylation, appear to represent different regulatory mechanisms of chromatin activity in several biological settings [8]. In this review we draw a detailed picture for the role of diverse epigenetic modifications in the development of RA with a special focus on epigenetic modifications in RASF.

DNA methylation

DNA methylation is the best understood epigenetic modification and in general is associated with gene silencing. DNA methylation is catalyzed by DNA methyltransferases (DNMTs) that use S-adenosyl-L-methionine as a methyl donor [7]. The predominant DNMT in somatic cells is DNMT1. In mammalian genomic DNA, position 5 of the cytosine residue within a cytosine-phosphate-guanine dinucleotide (CpG) motif is methylated, resulting in the formation of 5-methylcytosine [7]. Genomic DNA methylation is found throughout the genome. Most CpG islands, short CpG-rich regions, are generally unmethylated while the majority of CpG sites in non-CpG islands are methylated [9]. CpG islands are often located in the vicinity of transcription start sites of several genes [10], and the methylation status of these regulatory DNA sequences correlates with the transcriptional activity of the corresponding genes [7].

The findings of an aberrant expression of normally silenced retrotransposable L1 elements in RA but not OA tissues, and their inducibility by treatment of cultured SF with the demethylating agent 5-aza-2'-deoxycytidine, suggest a functional role for DNA hypomethylation in the pathogenesis of RA [11]. Based on this first indication, members of our group detected a global DNA hypomethylation in RASF and RA tissues, and normal SF cultured in a hypomethylating milieu acquired an activated phenotype similar to RASF [12]. The global DNA hypomethylation was shown to be due to an increased polyamine metabolism in RASF, resulting in decreased levels of S-adenosyl-L-methionine [13]. Furthermore, the expression of DNMT1 protein was shown to be decreased, accompanied by an increased expression of the proliferation marker proliferating cell nuclear antigen, in RASF compared with OASF [12]. A global DNA hypomethylation was also shown in peripheral blood mononuclear cells (PBMC) derived from RA patients compared with healthy controls. In RA-derived PBMC, however, the mRNA expression levels of DNMT1 were increased [14]. Besides global changes of DNA methylation patterns in RA, hypomethylation of promoter regions of single genes, such as for chemokine (C-X-C motif) ligand 12 (CXCL-12) were reported [15]. Recent publications demonstrated that even demethylation of a single CpG motif in the promoters of the IL-6 [16, 17] and IL-10 [18, 19] genes correlated with their expression levels, and therefore contributed to elevated cytokine levels during disease. On the other hand, some DNA regions can also be hypermethylated in RA. The promoter region of the death receptor 3 gene (DR3), a member of the apoptosis inducing Fas gene family, was shown to be hypermethylated in RA patients [20].

DNA methylation was also shown to regulate expression levels of miRNAs [21, 22]. miRNAs are a class of short noncoding RNAs that function as post-translational repressors of gene expression by binding to the 3' untranslated region of target genes. This leads to either degradation and/or inhibition of translation [23]. A screening of 260 individual miRNAs for differential expression in RASF and OASF revealed that an array of miRNAs is overexpressed in RASF. One of the most differentially expressed miRNAs in RASF compared with OASF was miR-203. By treating RASF with the demethylating agent 5-aza-2'-deoxycytidine, miR-203 expression was further induced. Overexpression of miR-203 in RASF resulted in an increased expression of MMP1 as well as increased IL-6 secretion rates [21]. One miRNA that is downregulated in RA and regulated by methylation of the promoter region is miR-34a*. Decreased expression of miR-34a* in RASF resulted in upregulation of its direct target X-linked inhibitor of apoptosis protein, and thereby contributed to the apoptosis resistant phenotype of RASF [22]. The future role of miRNAs in the pathogenesis of RA was recently reviewed by our laboratory [24].

Another study investigating the involvement of DNA methylation in the pathogenesis of RA came from a group investigating the silencing of the second × chromosome in females. This study revealed an increased presence of a skewed XCI pattern in RA patients. Instead of a random XCI, at least 80% of cells exhibited an inactivation of the same × chromosome [25]. These data suggest a role for XCI mosaicism in the pathogenesis of RA and might be one of the explanations for why RA is more prevalent in women.

Histone acetylation and deacetylation

The best studied histone modifications are the acetylation of specific lysine residues of histones H3 and H4. These modifications play a fundamental role in transcriptional regulation but also other processes such as replication. The acetylation state of a chromatin region is tightly controlled by two antagonizing enzyme families, namely histone acetylase transferases (HATs) and histone deacetylases (HDACs) [26]. Acetylation is generally associated with transcriptional activation. HATs such as lysine acetyltransferase 2A (GCN5), lysine acetyltransferase 2B (PCAF), CREB binding protein, p300, and lysine acetyltransferase 8 (MOF) often function as transcriptional co-activators. On the contrary, deacetylation of chromatin is linked to gene silencing and HDACs were identified as transcriptional co-repressors. In humans, 18 HDACs have been identified and they are further subgrouped into four classes based on their orthologous homology to yeast (Table 1). Class I HDACs include HDAC1, HDAC2, HDAC3 and HDAC8, whereas the group of class II HDACs comprises HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10. The seven sirtuins SIRT1 to SIRT7 build up the group of class III HDACs. HDAC11 is the only human enzyme classified as a class IV HDAC [26].

Table 1 Classification of histone deacetylases and their inhibitors

Most of the available data about the role of HDACs in the pathogenesis of RA were obtained using HDAC inhibitors (Table 1), first and foremost trichostatin A (TSA), an inhibitor of all class I and class II HDACs [27]. The lack of HDAC-specific inhibitors makes the inter-pretation of data difficult. Furthermore, one must bear in mind that HATs and HDACs target not only histones but also other proteins including transcription factors such as NF-κB and signal transducer and activator of transcription 3. These transcription factors also play key roles in inflammatory processes and are therefore also involved in pathogenic processes in RA.

TSA was shown to sensitize RASF for TNFα-related apoptosis-inducing ligand-induced apoptosis and to induce a cell cycle arrest by inducing the cell cycle regulator p21 [28]. A positive effect of the HDAC inhibitor FK228 on inhibiting RASF proliferation by induction of p21 and p16 promoter acetylation and expression was previously shown by Nishida and colleagues [29]. High doses of TSA and nicotinamide, a SIRT inhibitor, were also effective in inducing apoptosis in RA macrophages [30]. HDAC inhibitors might thus positively influence synovial hyperplasia in RA, on the one hand by inhibiting the activation of RASF and on the other by inducing apoptosis in macrophages.

Furthermore, these inhibitors also reduced the TNFα-induced production of IL-6 and the lipopolysaccharide-induced secretion of IL-6 and TNFα. Interestingly, the authors could not find a close association between the beneficial effects of HDAC inhibitors on cytokine production and the overall HDAC inhibitor-dependent histone H3 and H4 acetylation [30]. These results indicate that not all effects of HDAC inhibitors are related to histone modifications. Recently, Grabiec and colleagues identified one of the molecular mechanisms underlying the anti-inflammatory activity of HDAC inhibitors. The authors demonstrated that the HDAC inhibitors TSA and ITF2357 suppressed cytokine-induced and Toll-like receptor ligand-induced expression of IL-6 in RASF and macrophages by accelerating IL-6 mRNA decay. However, also other, not yet identified molecular mechanisms are involved in beneficial effects of HDAC inhibitors in RA, since the authors also stated that reduced mRNA stability did not explain the suppressive effects of cytokine-induced MMP1 and IL-8 expression in RASF [31].

Beneficial effects of HDAC inhibitors have been demonstrated in vivo by several groups using animal models of arthritis [29, 3235]. Since then, specific HDAC inhibitors have been continuously discussed as potential therapeutics not only in cancer but also in RA. Systemic administration of the HDAC inhibitor FK228 reduced joint swelling, synovial inflammation and subsequent bone and cartilage destruction in a mouse model of autoantibody-mediated arthritis [29]. The prophylactic and therapeutic effects of the HDAC inhibitors sub-eroylanilide hydroxamic acid and MS-275 were addressed in collagen-induced arthritis in rats and mice. This study also led to the conclusion that HDAC inhibitors are an innovative therapeutic strategy for RA [32]. In the same model, a dose-dependent effect of TSA was shown to reduce clinical signs of arthritis. The authors detected an increased number of cells with acetylated histone H4. Also, tissue inhibitor of MMP1 (TIMP1)-positive cells were increased, while the numbers of MMP3-positive and MMP13-positive chondrocytes were reduced as detected by immunohistochemical staining [33]. Furthermore, the HDAC inhibitor valproic acid significantly decreased disease incidence and severity in collagen-induced arthritis mice. These effects were mediated by improving the production and function of CD25+FOXP3+ regulatory T cells, whose function is defective in RA patients [34].

Although much data on beneficial effects of HDAC inhibitors have been reported, the role of individual HDACs in the development of RA and their functional role in SF have not been fully elucidated to date and are still a matter of debate. In analogy to the investigation of global changes of DNA methylation in RA tissues and RASF, research groups also aimed to verify potential global changes in histone acetylation in RA. Initially, diminished HDAC1 and HDAC2 expression and a reduced total HDAC activity were described in synovial tissues of RA compared with OA patients, implicating a shift towards histone hyperacetylation in patients with RA and pointing to a potential role of HDACs in the pathogenesis of RA [36]. Later, an increased expression of HDAC1 and HDAC2 in RASF compared with OASF was described. By silencing these enzymes in RASF, the authors could demonstrate a functional role of HDAC1 and HDAC2 on proliferation and susceptibility to apoptosis [37].

Kawabata and colleagues also investigated the total HDAC activity in nuclear extracts of OA and RA tissues. Controversial to previous reports, in this study an increased HDAC activity and an unchanged HAT activity in RA tissues compared with OA tissues was reported. Furthermore, a decreased mRNA expression of HDAC4 and an increased mRNA and protein expression of HDAC1 in RA tissues were shown. Interestingly, stimulation of RASF with TNFα specifically increased total HDAC activity and the expression of HDAC1 [38], suggesting that changes in synovial HDAC activity and expression may be influenced by the local inflammatory status [39].

A total increased HDAC activity was also reported recently in PBMC derived from RA patients, irrespective of whether or not patients were treated with the TNFα inhibitor etanercept. TSA and the HDAC3 selective inhibitor MI192 reduced lipopolysaccharide-stimulated cytokine production in PBMC derived from healthy donors and RA patients in a cell-type and compound-specific manner [40]. The significance of studies analyzing HDAC expression levels is currently quite unclear, since the environmental influences that lead to the variability in HDAC expression and activity in synovial tissues are far from being understood.

At present, only few reports exist showing specific changes in promoter histone acetylation in RA [29, 41]. Stimulation of RASF with TNFα and IL-1β induced the promoter acetylation of the cell cycle regulator p21 and correlated with RASF proliferation [29]. Furthermore, the promoter region of MMP1 was shown to be hyperacetylated in RASF and the authors could show a functional role for HDAC4 in this mechanism (see below) [41], being in line with the results from Kawabata and colleagues reporting a reduced HDAC4 expression in RA tissues [38].

The functional role of SIRTs in RA has been rarely studied to date [30, 42]. As already mentioned, Grabiec and colleagues showed a positive effect of SIRT inhibitors in macrophages [30]. Recently, SIRT1 was reported to be overexpressed in RASF and monocytes derived from RA patients and expression levels of SIRT1 were further increased in the presence of TNFα. Overexpression of SIRT1 contributed to the apoptosis resistance in RASF and promoted the proinflammatory cytokine production in monocytes [42]. The functional role of other members of the SIRT family has not yet been studied.

Histone methylation

Data on the contribution of histone modifications other than acetylation in the pathogenesis of RA are still rare, and the role of epigenetic methylation marks on lysine and/or arginine residues of histones is far less understood than the function of histone acetylation. The predominant histones modified by methylation are histones H3 and H4. Lysine residues can be monomethylated, dimethylated or trimethylated, adding yet another layer of complexity to this histone mark [8]. Depending on the methylated position, methylation of histones can be associated with more active or inactive chromatin regions. The trimethylation of histone 3 lysines H3K27m3, H3K9m3 and H4K20m3 are generally connected to transcriptionally silenced genes, whereas H3K36m3, H3K4m3 and H3K79m3 are associated with an open chromatin structure and transcriptionally active genes [43].

Our group addressed for the first time the role of histone methylation in RA and described an over-expression of the histone methyltransferase enhancer of zeste homologue 2 (EZH2) in RASF compared with OASF. EZH2 is the catalytic subunit of the polycomb repressor complex 2 that adds up to three methyl marks to H3K27 of genes designated for silencing [44]. EZH2 overexpression resulted in a reduced expression of the secreted frizzeled-related protein 1 (SFRP1), an inhibitor of the wingless-type MMTV integration site (Wnt) signaling pathway, which plays an important role in joint development and has been implicated in the pathogenesis of inflammatory arthropathies. However, the described mechanism seems to be specific for SFRP1 and elevated EZH2 expression levels did not correlate with global changes in H3K27me3 in RASF [45].


Another post-translational modification described in RA is sumoylation [46]. Members of our group showed that RASF have intrinsically high levels of the small ubiquitinlike modifier 1 paralleled by decreased levels of its specific protease SENP1. By overexpressing SENP1 in RASF, histone H4 acetylation in the MMP1 promoter was reduced, leading to a reduced invasiveness of RASF. The authors showed that SENP1 overexpression is also accompanied by a global reduction of histone H4 acetylation and that the mechanism depends on the presence of HDAC4 [41].


There is no doubt that epigenetic modifications affect RA. Several studies demonstrated that epigenetics contribute to the aggressive, intrinsic activated phenotype of RASF and also the first in vivo data point to the beneficial effects of drugs modifying epigenetic patterns in animal models. However, more data are needed to clarify which modifications are causative for the disease onset and which epigenetic changes occur during chronic disease states. Whereas the picture of a differential pattern of DNA methylation in RA becomes clearer, other epigenetic modifications in RA have not yet been studied at all, or their functional role in the pathogenesis of RA is still puzzling. Understanding the complexity of different epigenetic modifications that regulate gene expression in cooperation and understanding the influence of environmental factors and current therapies on epigenetic modifications is just at the beginning. The available data raise the hope that, in future, detailed knowledge of epigenetic regulatory mechanisms in RA will help to develop new treatment strategies or will predict treatment efficacy and treatment outcome.


This article is part of the series on Epigenetics and rheumatic diseases, edited by Nan Shen. Other articles in this series can be found at



antibodies against citrullinated peptide antigens


cytosine-phosphate-guanine dinucleotide


DNA methyltransferase


enhancer of zeste homologue 2


histone 3 lysine


histone acetylase transferase


histone deacetylase






matrix metalloproteinase


nuclear factor kappa-light-chain-enhancer of activated B cells




peripheral blood mononuclear cells


rheumatoid arthritis


small ubiquitin-like modifier 1-specific protease 1


synovial fibroblasts


secreted frizzeled-related protein 1




tumor necrosis factor


trichostatin A


wingless-type MMTV integration site


X-chromosome inactivation.


  1. Smolen J, Aletaha D: The burden of rheumatoid arthritis and access to treatment: a medical overview. Eur J Health Econ. 2008, 8 (Suppl 2): S39-S47.

    Article  PubMed  Google Scholar 

  2. Padyukov L, Seielstad M, Ong RT, Ding B, Ronnelid J, Seddighzadeh M, Alfredsson L, Klareskog L: A genome-wide association study suggests contrasting associations in ACPA-positive versus ACPA-negative rheumatoid arthritis. Ann Rheum Dis. 2011, 70: 259-265. 10.1136/ard.2009.126821.

    Article  PubMed Central  PubMed  Google Scholar 

  3. Saevarsdottir S, Wallin H, Seddighzadeh M, Ernestam S, Geborek P, Petersson IF, Bratt J, van Vollenhoven RF: Predictors of response to methotrexate in early DMARD naive rheumatoid arthritis: results from the initial open-label phase of the SWEFOT trial. Ann Rheum Dis. 2011, 70: 469-475. 10.1136/ard.2010.139212.

    Article  PubMed  Google Scholar 

  4. Lefèvre S, Knedla A, Tennie C, Kampmann A, Wunrau C, Dinser R, Korb A, Schnaker EM, Tarner IH, Robbins PD, Evans CH, Stürz H, Steinmeyer J, Gay S, Schölmerich J, Pap T, Müller-Ladner U, Neumann E: Synovial fibroblasts spread rheumatoid arthritis to unaffected joints. Nat Med. 2009, 15: 1414-1420. 10.1038/nm.2050.

    Article  PubMed Central  PubMed  Google Scholar 

  5. Ospelt C, Gay S: The role of resident synovial cells in destructive arthritis. Best Pract Res Clin Rheumatol. 2008, 22: 239-252. 10.1016/j.berh.2008.01.004.

    Article  CAS  PubMed  Google Scholar 

  6. Bird A: Perceptions of epigenetics. Nature. 2007, 447: 396-398. 10.1038/nature05913.

    Article  CAS  PubMed  Google Scholar 

  7. Turek-Plewa J, Jagodzinski PP: The role of mammalian DNA methyltransferases in the regulation of gene expression. Cell Mol Biol Lett. 2005, 10: 631-647.

    CAS  PubMed  Google Scholar 

  8. Strahl BD, Allis CD: The language of covalent histone modifications. Nature. 2000, 403: 41-45. 10.1038/47412.

    Article  CAS  PubMed  Google Scholar 

  9. Suzuki MM, Bird A: DNA methylation landscapes: provocative insights from epigenomics. Nat Rev Genet. 2008, 9: 465-476.

    Article  CAS  PubMed  Google Scholar 

  10. Jones PA: Functions of DNA methylation: islands, start sites, gene bodies and beyond. Nat Rev Genet. 2012, 13: 484-492. 10.1038/nrg3230.

    Article  CAS  PubMed  Google Scholar 

  11. Neidhart M, Rethage J, Kuchen S, Kunzler P, Crowl RM, Billingham ME, Gay RE, Gay S: Retrotransposable L1 elements expressed in rheumatoid arthritis synovial tissue: association with genomic DNA hypomethylation and influence on gene expression. Arthritis Rheum. 2000, 43: 2634-2647. 10.1002/1529-0131(200012)43:12<2634::AID-ANR3>3.0.CO;2-1.

    Article  CAS  PubMed  Google Scholar 

  12. Karouzakis E, Gay RE, Michel BA, Gay S, Neidhart M: DNA hypomethylation in rheumatoid arthritis synovial fibroblasts. Arthritis Rheum. 2009, 60: 3613-3622. 10.1002/art.25018.

    Article  CAS  PubMed  Google Scholar 

  13. Karouzakis E, Gay RE, Gay S, Neidhart M: Increased recycling of polyamines is associated with global DNA hypomethylation in rheumatoid arthritis synovial fibroblasts. Arthritis Rheum. 2012, 64: 1809-1817. 10.1002/art.34340.

    Article  CAS  PubMed  Google Scholar 

  14. Liu CC, Fang TJ, Ou TT, Wu CC, Li RN, Lin YC, Lin CH, Tsai WC, Liu HW, Yen JH: Global DNA methylation, DNMT1, and MBD2 in patients with rheumatoid arthritis. Immunol Lett. 2011, 135: 96-99. 10.1016/j.imlet.2010.10.003.

    Article  CAS  PubMed  Google Scholar 

  15. Karouzakis E, Rengel Y, Jungel A, Kolling C, Gay RE, Michel BA, Tak PP, Gay S, Neidhart M, Ospelt C: DNA methylation regulates the expression of CXCL12 in rheumatoid arthritis synovial fibroblasts. Genes Immun. 2011, 12: 643-652. 10.1038/gene.2011.45.

    Article  CAS  PubMed  Google Scholar 

  16. Nile CJ, Read RC, Akil M, Duff GW, Wilson AG: Methylation status of a single CpG site in the IL6 promoter is related to IL6 messenger RNA levels and rheumatoid arthritis. Arthritis Rheum. 2008, 58: 2686-2693. 10.1002/art.23758.

    Article  PubMed  Google Scholar 

  17. Ishida K, Kobayashi T, Ito S, Komatsu Y, Yokoyama T, Okada M, Abe A, Murasawa A, Yoshie H: Interleukin-6 gene promoter methylation in rheumatoid arthritis and chronic periodontitis. J Periodontol. 2012, 83: 917-925. 10.1902/jop.2011.110356.

    Article  CAS  PubMed  Google Scholar 

  18. Fu LH, Ma CL, Cong B, Li SJ, Chen HY, Zhang JG: Hypomethylation of proximal CpG motif of interleukin-10 promoter regulates its expression in human rheumatoid arthritis. Acta Pharmacol Sin. 2011, 32: 1373-1380. 10.1038/aps.2011.98.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  19. Lin SY, Hsieh SC, Lin YC, Lee CN, Tsai MH, Lai LC, Chuang EY, Chen PC, Hung CC, Chen LY, Hsieh WS, Niu DM, Su YN, Ho HN: A whole genome methylation analysis of systemic lupus erythematosus: hypomethylation of the IL10 and IL1R2 promoters is associated with disease activity. Genes Immun. 2012, 13: 214-220. 10.1038/gene.2011.74.

    Article  CAS  PubMed  Google Scholar 

  20. Takami N, Osawa K, Miura Y, Komai K, Taniguchi M, Shiraishi M, Sato K, Iguchi T, Shiozawa K, Hashiramoto A, Shiozawa S: Hypermethylated promoter region of DR3, the death receptor 3 gene, in rheumatoid arthritis synovial cells. Arthritis Rheum. 2006, 54: 779-787. 10.1002/art.21637.

    Article  CAS  PubMed  Google Scholar 

  21. Stanczyk J, Ospelt C, Karouzakis E, Filer A, Raza K, Kolling C, Gay R, Buckley CD, Tak PP, Gay S, Kyburz D: Altered expression of microRNA-203 in rheumatoid arthritis synovial fibroblasts and its role in fibroblast activation. Arthritis Rheum. 2011, 63: 373-381. 10.1002/art.30115.

    Article  PubMed Central  PubMed  Google Scholar 

  22. Niederer F, Trenkmann M, Ospelt C, Karouzakis E, Neidhart M, Stanczyk J, Kolling C, Gay RE, Detmar M, Gay S, Jüngel A, Kyburz D: Downregulation of microRNA-34a* in rheumatoid arthritis synovial fibroblasts promotes apoptosis resistance. Arthritis Rheum. 2012, 64: 1771-1779. 10.1002/art.34334.

    Article  CAS  PubMed  Google Scholar 

  23. Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004, 116: 281-297. 10.1016/S0092-8674(04)00045-5.

    Article  CAS  PubMed  Google Scholar 

  24. Filkova M, Jungel A, Gay RE, Gay S: MicroRNAs in rheumatoid arthritis: potential role in diagnosis and therapy. BioDrugs. 2012, 26: 131-141. 10.2165/11631480-000000000-00000.

    Article  CAS  PubMed  Google Scholar 

  25. Chabchoub G, Uz E, Maalej A, Mustafa CA, Rebai A, Mnif M, Bahloul Z, Farid NR, Ozcelik T, Ayadi H: Analysis of skewed X-chromosome inactivation in females with rheumatoid arthritis and autoimmune thyroid diseases. Arthritis Res Ther. 2009, 11: R106-10.1186/ar2759.

    Article  PubMed Central  PubMed  Google Scholar 

  26. Wang Z, Zang C, Cui K, Schones DE, Barski A, Peng W, Zhao K: Genome-wide mapping of HATs and HDACs reveals distinct functions in active and inactive genes. Cell. 2009, 138: 1019-1031. 10.1016/j.cell.2009.06.049.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  27. Vigushin DM, Ali S, Pace PE, Mirsaidi N, Ito K, Adcock I, Coombes RC: Trichostatin A is a histone deacetylase inhibitor with potent antitumor activity against breast cancer in vivo. Clin Cancer Res. 2001, 7: 971-976.

    CAS  PubMed  Google Scholar 

  28. Jungel A, Baresova V, Ospelt C, Simmen BR, Michel BA, Gay RE, Gay S, Seemayer CA, Neidhart M: Trichostatin A sensitises rheumatoid arthritis synovial fibroblasts for TRAIL-induced apoptosis. Ann Rheum Dis. 2006, 65: 910-912.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Nishida K, Komiyama T, Miyazawa S, Shen ZN, Furumatsu T, Doi H, Yoshida A, Yamana J, Yamamura M, Ninomiya Y, Inoue H, Asahara H: Histone deacetylase inhibitor suppression of autoantibody-mediated arthritis in mice via regulation of p16INK4a and p21(WAF1/Cip1) expression. Arthritis Rheum. 2004, 50: 3365-3376. 10.1002/art.20709.

    Article  CAS  PubMed  Google Scholar 

  30. Grabiec AM, Krausz S, de Jager W, Burakowski T, Groot D, Sanders ME, Prakken BJ, Maslinski W, Eldering E, Tak PP, Reedquist KA: Histone deacetylase inhibitors suppress inflammatory activation of rheumatoid arthritis patient synovial macrophages and tissue. J Immunol. 2010, 184: 2718-2728. 10.4049/jimmunol.0901467.

    Article  CAS  PubMed  Google Scholar 

  31. Grabiec AM, Korchynskyi O, Tak PP, Reedquist KA: Histone deacetylase inhibitors suppress rheumatoid arthritis fibroblast-like synoviocyte and macrophage IL-6 production by accelerating mRNA decay. Ann Rheum Dis. 2012, 71: 424-431. 10.1136/ard.2011.154211.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  32. Lin HS, Hu CY, Chan HY, Liew YY, Huang HP, Lepescheux L, Bastianelli E, Baron R, Rawadi G, Clement-Lacroix P: Anti-rheumatic activities of histone deacetylase (HDAC) inhibitors in vivo in collagen-induced arthritis in rodents. Br J Pharmacol. 2007, 150: 862-872. 10.1038/sj.bjp.0707165.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  33. Nasu Y, Nishida K, Miyazawa S, Komiyama T, Kadota Y, Abe N, Yoshida A, Hirohata S, Ohtsuka A, Ozaki T: Trichostatin A, a histone deacetylase inhibitor, suppresses synovial inflammation and subsequent cartilage destruction in a collagen antibody-induced arthritis mouse model. Osteoarthritis Cartilage. 2008, 16: 723-732. 10.1016/j.joca.2007.10.014.

    Article  CAS  PubMed  Google Scholar 

  34. Saouaf SJ, Li B, Zhang G, Shen Y, Furuuchi N, Hancock WW, Greene MI: Deacetylase inhibition increases regulatory T cell function and decreases incidence and severity of collagen-induced arthritis. Exp Mol Pathol. 2009, 87: 99-104. 10.1016/j.yexmp.2009.06.003.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  35. Joosten LA, Leoni F, Meghji S, Mascagni P: Inhibition of HDAC activity by ITF2357 ameliorates joint inflammation and prevents cartilage and bone destruction in experimental arthritis. Mol Med. 2011, 17: 391-396. 10.1007/s00894-010-0739-z.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  36. Huber LC, Brock M, Hemmatazad H, Giger OT, Moritz F, Trenkmann M, Distler JH, Gay RE, Kolling C, Moch H, Michel BA, Gay S, Distler O, Jüngel A: Histone deacetylase/acetylase activity in total synovial tissue derived from rheumatoid arthritis and osteoarthritis patients. Arthritis Rheum. 2007, 56: 1087-1093. 10.1002/art.22512.

    Article  CAS  PubMed  Google Scholar 

  37. Horiuchi M, Morinobu A, Chin T, Sakai Y, Kurosaka M, Kumagai S: Expression and function of histone deacetylases in rheumatoid arthritis synovial fibroblasts. J Rheumatol. 2009, 36: 1580-1589. 10.3899/jrheum.081115.

    Article  CAS  PubMed  Google Scholar 

  38. Kawabata T, Nishida K, Takasugi K, Ogawa H, Sada K, Kadota Y, Inagaki J, Hirohata S, Ninomiya Y, Makino H: Increased activity and expression of histone deacetylase 1 in relation to tumor necrosis factor-alpha in synovial tissue of rheumatoid arthritis. Arthritis Res Ther. 2010, 12: R133-10.1186/ar3071.

    Article  PubMed Central  PubMed  Google Scholar 

  39. Grabiec AM, Reedquist KA: Histone deacetylases in RA: epigenetics and epiphenomena. Arthritis Res Ther. 2010, 12: 142-10.1186/ar3137.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  40. Gillespie J, Savic S, Wong C, Hempshall A, Inman M, Emery P, Grigg R, McDermott MF: Histone deacetylases are dysregulated in rheumatoid arthritis and a novel histone deacetylase 3-selective inhibitor reduces interleukin-6 production by peripheral blood mononuclear cells from rheumatoid arthritis patients. Arthritis Rheum. 2012, 64: 418-422. 10.1002/art.33382.

    Article  CAS  PubMed  Google Scholar 

  41. Maciejewska-Rodrigues H, Karouzakis E, Strietholt S, Hemmatazad H, Neidhart M, Ospelt C, Gay RE, Michel BA, Pap T, Gay S, Jüngel A: Epigenetics and rheumatoid arthritis: the role of SENP1 in the regulation of MMP-1 expression. J Autoimmun. 2010, 35: 15-22. 10.1016/j.jaut.2009.12.010.

    Article  CAS  PubMed  Google Scholar 

  42. Niederer F, Ospelt C, Brentano F, Hottiger MO, Gay RE, Gay S, Detmar M, Kyburz D: SIRT1 overexpression in the rheumatoid arthritis synovium contributes to proinflammatory cytokine production and apoptosis resistance. Ann Rheum Dis. 2011, 70: 1866-1873. 10.1136/ard.2010.148957.

    Article  CAS  PubMed  Google Scholar 

  43. Kouzarides T: Chromatin modifications and their function. Cell. 2007, 128: 693-705. 10.1016/j.cell.2007.02.005.

    Article  CAS  PubMed  Google Scholar 

  44. Margueron R, Reinberg D: The Polycomb complex PRC2 and its mark in life. Nature. 2011, 469: 343-349. 10.1038/nature09784.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  45. Trenkmann M, Brock M, Gay RE, Kolling C, Speich R, Michel BA, Gay S, Huber LC: Expression and function of EZH2 in synovial fibroblasts: epigenetic repression of the Wnt inhibitor SFRP1 in rheumatoid arthritis. Ann Rheum Dis. 2011, 70: 1482-1488. 10.1136/ard.2010.143040.

    Article  CAS  PubMed  Google Scholar 

  46. Meinecke I, Cinski A, Baier A, Peters MA, Dankbar B, Wille A, Drynda A, Mendoza H, Gay RE, Hay RT, Ink B, Gay S, Pap T: Modification of nuclear PML protein by SUMO-1 regulates Fas-induced apoptosis in rheumatoid arthritis synovial fibroblasts. Proc Natl Acad Sci USA. 2007, 104: 5073-5078. 10.1073/pnas.0608773104.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  47. Marks PA, Breslow R: Dimethyl sulfoxide to vorinostat: development of this histone deacetylase inhibitor as an anticancer drug. Nat Biotechnol. 2007, 25: 84-90. 10.1038/nbt1272.

    Article  CAS  PubMed  Google Scholar 

  48. Furumai R, Matsuyama A, Kobashi N, Lee KH, Nishiyama M, Nakajima H, Tanaka A, Komatsu Y, Nishino N, Yoshida M, Horinouchi S: FK228 (depsipeptide) as a natural prodrug that inhibits class I histone deacetylases. Cancer Res. 2002, 62: 4916-4921.

    CAS  PubMed  Google Scholar 

  49. Golay J, Cuppini L, Leoni F, Mico C, Barbui V, Domenghini M, Lombardi L, Neri A, Barbui AM, Salvi A, Pozzi P, Porro G, Pagani P, Fossati G, Mascagni P, Introna M, Rambaldi A: The histone deacetylase inhibitor ITF2357 has antileukemic activity in vitro and in vivo and inhibits IL-6 and VEGF production by stromal cells. Leukemia. 2007, 21: 1892-1900. 10.1038/sj.leu.2404860.

    Article  CAS  PubMed  Google Scholar 

  50. Gottlicher M, Minucci S, Zhu P, Kramer OH, Schimpf A, Giavara S, Sleeman JP, Lo Coco F, Nervi C, Pelicci PG, Heinzel T: Valproic acid defines a novel class of HDAC inhibitors inducing differentiation of transformed cells. EMBO J. 2001, 20: 6969-6978. 10.1093/emboj/20.24.6969.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  51. Saito A, Yamashita T, Mariko Y, Nosaka Y, Tsuchiya K, Ando T, Suzuki T, Tsuruo T, Nakanishi O: A synthetic inhibitor of histone deacetylase, MS-27-275, with marked in vivo antitumor activity against human tumors. Proc Natl Acad Sci USA. 1999, 96: 4592-4597. 10.1073/pnas.96.8.4592.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  52. Giammona LM, Panuganti S, Kemper JM, Apostolidis PA, Lindsey S, Papoutsakis ET, Miller WM: Mechanistic studies on the effects of nicotinamide on megakaryocytic polyploidization and the roles of NAD+ levels and SIRT inhibition. Exp Hematol. 2009, 37: 1340-1352. 10.1016/j.exphem.2009.08.004.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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This research is supported by FP7 Masterswitch, IMI-BT Cure, and IAR Epalinges.

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Correspondence to Kerstin Klein.

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Klein, K., Ospelt, C. & Gay, S. Epigenetic contributions in the development of rheumatoid arthritis . Arthritis Res Ther 14, 227 (2012).

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