Differential recovery of glycosaminoglycan after IL-1-induced degradation of bovine articular cartilage depends on degree of degradation

Arthritic diseases are characterized by a progressive loss of extracellular matrix (ECM), which may ultimately lead to frank tissue loss and gross joint damage. The conditions under which chondrocytes can be induced to replenish or repair a depleted matrix (and thereby prevent or slow the disease process) remain unclear. If these conditions were known, then in principal one could design a therapeutic strategy that takes into account the capacity (or absence thereof) for chondrocytes to repair their matrix naturally.

A number of model systems have provided some insights. For instance, IL-1-degraded cartilage tissues and cell suspensions are frequently employed as model systems to study cartilage metabolism under osteoarthritis-like conditions [1–4] and to assess the efficacy of various drug therapies [5–9]. IL-1 signals cells via cell surface receptors to upregulate proteases, matrix metalloproteinases and aggrecanase, while downregulating matrix metalloproteinase inhibition and proteoglycan synthesis [10, 11]. The ultimate effect of these metabolic changes include damage to the ECM, loss of ECM components (especially proteoglycan), and alteration of the normal balance of catabolic and anabolic processes that regulate cartilage ECM composition.

With regard to the repair phase, following IL-1-induced cartilage damage, relatively little is known, but partial to complete recovery appears possible [1, 3]. In studies of repair, investigators focused on glycosaminoglycan (GAG) – an important and abundant cartilage macromolecule - and used histologic, biochemical, and/or radiolabel incorporation methods to measure GAG concentration ([GAG]) and/or GAG synthesis rates at selected time points. Although these data suggest that repair might be possible, our understanding is limited because of the uncertainty in the temporal changes in matrix composition for a given sample. This uncertainty is a direct consequence of the inherently destructive nature of histologic and biochemical methods; these techniques therefore do not permit monitoring of the spatial and temporal evolution of matrix macromolecules within individual samples/animals. Having to infer temporal spatial information from a cross-section of samples confounds efforts to conduct a detailed analysis of responses in these model systems.

The delayed Gadolinium-Enhanced Magnetic Resonance Imaging of Cartilage (dGEMRIC) method overcomes these problems in that it is nondestructive and can be used to assess quantitatively the spatial distribution of cartilage [GAG] [12, 13]. We and others previously reported on the use of dGEMRIC to monitor GAG regeneration in living cartilage tissue over time in trypsin-depleted bovine cartilage [14] and in cell-laden polymer matrices (tissue engineered cartilage) [15]. Those studies clearly demonstrated that dGEMRIC is feasible for long-term [GAG] monitoring, and that its sensitivity is sufficient to permit observation of the spatiotemporal evolution of [GAG]. Each of those studies began with a homogeneously proteoglycan-deficient matrix, in which GAG was replenished over a 5- to 8-week period to levels considered to be within the normal range.

In young bovine articular cartilage (a tissue that many investigators have used to investigate the impact of physical and biochemical factors on ECM metabolism [14, 16–21]), IL-1 induces a characteristic spatially heterogeneous depletion of GAG. This young tissue still contains blood vessels, and it is in the perivascular regions that IL-1-induced GAG depletion is most severe. We know from studies investigating the recovery of trypsin-induced homogeneous GAG depletion in young bovine cartilage that the inherent ability of chondrocytes to replenish the matrix is relatively uniform [14]. In the present study, we take advantage of the characteristic spatial heterogeneity of IL-1-induced GAG depletion to study the relative replenishment rates between regions of varying severity of GAG depletion. Accordingly, our goal was to use dGEMRIC to determine whether bovine cartilage explants briefly exposed to IL-1 would recover GAG at a uniform rate, or at a rate that depended on the degree of IL-1-induced GAG depletion. Specifically, we sought to examine recovery after relatively mild and modest degradation – conditions that did not induce a complete loss of tissue GAG.

The present study clearly demonstrates that bovine cartilage explants can, at least partially, recover from IL-1-induced degradation by synthesizing new GAG but that the ultimate rate of recovery may be dependent on the degree of initial depletion. By monitoring the spatially localized changes in [GAG] over a 3-week recovery period, we showed that [GAG] increases significantly with time in post-IL-1-exposure culture, with the early recovery (first 2 weeks) being independent of absolute [GAG] but the later recovery (third week) occurring only in regions with higher [GAG].

With respect to the spatial heterogeneity in recovery rates, we are not aware of any histologic (or other) data describing the apparent dependence of the rate of [GAG] replenishment on the initial state of the ECM. We previously showed that spontaneous recovery from complete trypsin-induced GAG loss occurs uniformly throughout the tissue, showing no significant spatial heterogeneity in recovery rates and nearly complete recovery to the initial state in approximately 5 weeks [14]. Those data suggest that the capacity for cells to synthesize new matrix is uniform throughout the tissue. Thus, the differential response seen here is presumably due to the state of the ECM immediately after IL-1 exposure.

Here, we consider [GAG] as a surrogate for defining the ECM state immediately following IL-1 exposure. [GAG] itself is one direct measure of ECM state. In the context of the present study, [GAG] might also serve as a surrogate for the state of other ECM macromolecules, such as collagen. Given the broad spectrum of IL-1-induced enzymes, we consider that regions of the tissue where [GAG] is more severely affected by IL-1 may also be regions that experience greater damage to the collagen network, as compared with regions that are more resistant to the effects of IL-1. Our finding that the regions that experienced the least recovery were those most severely affected by IL-1 could be a consequence of these same regions sustaining more significant damage to the ECM network. This conclusion is consistent with findings regarding IL-1-induced degradation in rabbits, in which recovery rates decreased with apparent severity of degradation [1].

Interestingly, it is in the latter phase of recovery that a discrepancy in recovery rates becomes evident. During the early phases of recovery, the rate of [GAG] accumulation in the regions with the lowest [GAG] was comparable to the rates of recovery in regions with the highest [GAG]. The mechanism for this heterogeneity is unclear. It could be a manifestation of a corresponding distribution of IL-1-induced changes in chondrocyte metabolism or viability [23], or it could be a manifestation of a corresponding distribution of damage to the collagen scaffold, which in turn limits the ability to replenish [GAG]. Kruijsen et al. [24] showed that both the severity and chronicity of antigen-induced inflammation determined the degree of chondrocyte killing in their in vivo murine model of arthritis. Their studies showed that chondrocyte death was most highly correlated with the degree of joint inflammation present 14 days after arthritis induction. That finding suggests that sustained exposure to IL-1, a proinflammatory agent, may also cause chondrocyte death. However, the fact that the early recovery phase in the present study showed no heterogeneity makes cell death a less likely cause of limited [GAG] replenishment.

A limitation (and obvious next step) to this study is that we do not have independent information about the integrity of the collagen matrix. Magnetic resonance imaging techniques are actively being developed to image the collagen component of tissue, which can be incorporated into future studies [25]. Human osteoarthritic tissue is, by histologic measures, spatially heterogeneous in both collagen damage and degree of proteoglycan depletion. The notion that the differential ability to replenish GAG fully is related to the differential state of the collagen matrix has been suggested by others, based in part on the finding that GAG depletion corresponds with regions that are positive for the col3/4 epitope, which is indicative of collagen damage [6, 26]. Although we lack specific information on the state of the collagen matrix, the differences observed in the present study (and, indeed, the demonstrated ability to evaluate regional differences in response) suggest that dGEMRIC and model systems such as these may be useful for establishing a better understanding of the capacity of cartilage to repair osteoarthritis-like degradation.

The rate of GAG replenishment observed in this study compares well with published synthesis data. Using sulfate incorporation over periods of less than 24 hours, we and others have reported sulfate incorporation ratios between 0.06 and 0.13nmol/mg wet-weight/hour for young bovine cartilage [27–29]. These incorporation rates imply GAG synthesis rates of 6–13 mg/ml tissue water per week (assuming 1 sulfate per disaccharide, 502 g/mol disaccaride, and 0.8ml tissue water/g wet-weight). By comparison, we observed GAG accumulation rates of 4–14mg/ml per week, as inferred from the change in [GAG]. These values also compare well with the 2–7.3mg/ml per week rates of GAG replenishment seen in young bovine cartilage explants recovering from trypsin-induced GAG depletion [14]. Comparison of these GAG accumulation rates with the rate of GAG release into the culture medium clearly suggests that at least 75% of the newly synthesized GAG is retained by the tissue. (By contrast, in control tissue the amount of GAG synthesized is roughly equivalent to the amount released into the medium.)

We do not have the ability to determine the regional variations in synthesis and loss. Although we clearly observed regional variations in [GAG] accumulation, it is important to appreciate that these differences could arise by regional differences in synthesis or in loss, or both.

Looking more generally at attempts to evaluate [GAG] recovery in IL-1-degraded cartilage, our data are consistent with the temporal progression seen in other model systems in which recovery occurs and is measurable within the first few weeks after a [GAG]-depleting intervention. For example, Takegami et al. [9] reported [GAG] recovery in alginate cultures of human intervertebral disc cells pre-exposed to 0.5ng/ml IL-1 for 3 days. During the first 2 weeks of post-IL-1-exposure culture, those investigators observed [GAG] recovery rates of approximately 4%/day with very little change in [GAG] observed during the third week, when [GAG] levels reached about 85% of the control level. In an in vivo rabbit knee joint subjected to intra-articular injections of IL-1, Page Thomas et al. [3] used SO₄ uptake and toluidine blue staining to observe GAG losses of 25–60% in several cartilage sites within the knee, with gradual recovery over the subsequent 3–4 weeks. Arner [1] also examined in vivo GAG synthesis and accumulation in rabbits following intra-articular injections of IL-1. Using dimethylmethylene blue assay and sulfate incorporation, Arner found that both single and multiple injections of IL-1 led to an initial depression in GAG synthesis rate and a slight drop in tissue [GAG] for 4 days after IL-1 exposure. These changes were followed by enhanced synthesis (relative to controls), with a commensurate increase in tissue [GAG] over the subsequent 2 weeks as the content of GAG in the tissue approached 90% of control levels. In those in vivo systems it appears that GAG loss continues for 4–7 days after IL-1 exposure [1]. This is longer than the 1–2 days seen in the present study (in which GAG loss into the medium returned to control levels), and is probably a consequence of IL-1 clearing more quickly from the in vitro environment.

Thus, in widely different model systems – including that reported here – it appears that after an IL-1-induced GAG-depleting intervention tissue can reaccumulate GAG and does so most rapidly during the first few weeks. However, unlike the study described here, in which both spatial and temporal changes in [GAG] were monitored, in the studies described above it was not possible to derive more specific spatial information because the destructive nature of the [GAG] measurements required that time course information be inferred from averages of separate animals/samples harvested at different time points.

Our data can also be compared with those from other studies in which magnetic resonance methods were used to monitor changes in cartilage tissue during culture. Our group previously observed spatially uniform recovery of explants following trypsin-induced GAG depletion using dGEMRIC, with the increases in [GAG] occurring most rapidly during the first week and slowing considerably after 3 weeks [14]. Williams et al. [15] used the same method to monitor GAG accumulation in tissue engineered cartilage over 6 weeks, and observed relatively steady GAG accumulation over the entire period, with the bulk of the accumulation occurring at the periphery of the explant. The initial state of the cell/polymer construct was presumably uniform, and the heterogeneous [GAG] accumulation was attributed to differences in the biophysical environment. Potter et al. [30] observed the growth of tissue engineered over a period of 4 weeks using proton NMR without any additional contrast agent. The relative changes in T1 and T2 times of those studies tracked the histologic finding that GAG increased for the first 3 weeks and then remained relatively constant. Collectively, those studies and the present one illustrate spatial and temporal variations in GAG accumulation in native, treated, and tissue engineered cartilage. Much work, of course, remains if we are to begin to understand the biochemical, biophysical, and structural factors that underlie the differential behavior. Furthermore, much work remains to determine the generalizability of the behavior in these model systems involving young tissue to behavior of cartilage in vivo in older humans.

In the present study we demonstrated that chondrocytes, in a matrix degraded by IL-1 exposure, partially replenished the GAG, and the most severely degraded regions replenished less fully than did other regions. Future studies are underway to examine whether heterogeneity in replenishment rate is seen in human osteoarthritic tissue. The study provides additional evidence that the in vitro dGEMRIC method is a practical means for studying GAG homeostasis and events that disturb it. Ultimately, such studies provide the foundation for evaluating the effects of therapeutic interventions on [GAG] degradation or regeneration in vitro or in vivo [13].