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Pentosidine in serum and synovial fluid in patients with knee osteoarthritis and its potential role of prediction of osteoarthritis progression

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Background

The role of molecular markers for prediction of osteoarthritis (OA) progression is not yet defined. Pentosidine, one of the well-characterized advanced glycation endproducts, may be a candidate.

Objectives

To study the role of pentosidine as a marker of knee OA, as a marker of progression of knee OA, and for correlation of pentosidine in serum and synovial fluid and correlation with other markers.

Methods

Pentosidine was estimated by our own HLPC method [1], and cartilage digomeric matrix protein (COMP) by an original sandwich ELISA with monoclonal antibodies 16-F 12 and 17 C 10 [2]. MMP-9, TIMP and YKL-40 were estimated by commercial ELISA kits. Joint space width was measured in the narrowest point of the tibiofemoral compartment by 0.1 caliper and magnifying glass.

Results

Eighty-nine patients with knee OA were included in the study and were followed for 2 years. The paired samples of serum and synovial fluid were obtained from 48 patients in second study. The joint space narrowing in 2 years was 0.4 ± 0.79 mm. The patients with knee OA had higher initial serum values of pentosidine than controls (P = 0.04) and also of TIMP (P = 0.04), MMP-9 (P = 0.02) and COMP (P = 0.05). The patients with initially higher serum levels of pentosidine had more rapid radiological progression than controls (r = 0.30), and the same was true for hyaluronic acid (r = 0.56). Serum pentosidine levels correlated with synovial fluid pentosidine levels (r2 = 0.78). Mild correlation occurred between synovial fluid pentosidine and COMP levels (r2 = 0.11, P < 0.05).

Conclusions

Serum pentosidine levels may be a new molecular marker for prediction of OA progression.

References

  1. 1.

    Špacek P, Adam M: Pentosidin HPLC determination in body fluids and tissues as a marker glycation and oxidation loading of the organism in osteoarthritis. Osteoarthritis Cartil.

  2. 2.

    Vilím V, Obùrka Z, Vytášek R, Šenolt L, Tchetverikov I, Kraus VB, Pavelka K: Monoclonal antibodies to human cartilage oligomeric matrix protein (comp): epitope mapping and characterization of sandwich ELISA. Clin Chim Acta. 2002, 8019: 1-11.

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Acknowledgement

Supported by grant NK/5366-4 IGA from the Ministry of Health Czech Republic.

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Pavelka, K., Senolt, L., Vilim, V. et al. Pentosidine in serum and synovial fluid in patients with knee osteoarthritis and its potential role of prediction of osteoarthritis progression. Arthritis Res Ther 5, 167 (2003) doi:10.1186/ar968

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Keywords

  • Hyaluronic Acid
  • Synovial Fluid
  • Joint Space
  • Joint Space Narrowing
  • Joint Space Width