Microbiome dysbiosis is associated with disease duration and increased inflammatory gene expression in systemic sclerosis skin
Arthritis Research & Therapy volume 21, Article number: 49 (2019)
Infectious agents have long been postulated to be disease triggers for systemic sclerosis (SSc), but a definitive link has not been found. Metagenomic analyses of high-throughput data allows for the unbiased identification of potential microbiome pathogens in skin biopsies of SSc patients and allows insight into the relationship with host gene expression.
We examined skin biopsies from a diverse cohort of 23 SSc patients (including lesional forearm and non-lesional back samples) by RNA-seq. Metagenomic filtering and annotation was performed using the Integrated Metagenomic Sequencing Analysis (IMSA). Associations between microbiome composition and gene expression were analyzed using single-sample gene set enrichment analysis (ssGSEA).
We find the skin of SSc patients exhibits substantial changes in microbial composition relative to controls, characterized by sharp decreases in lipophilic taxa, such as Propionibacterium, combined with increases in a wide range of gram-negative taxa, including Burkholderia, Citrobacter, and Vibrio.
Microbiome dysbiosis is associated with disease duration and increased inflammatory gene expression. These data provide a comprehensive portrait of the SSc skin microbiome and its association with local gene expression, which mirrors the molecular changes in lesional skin.
Systemic sclerosis (SSc) is a progressive autoimmune disease that results in inflammation, fibrosis, and dysfunction of multiple organ systems including the skin, lungs, gastrointestinal tract, and blood vessels. Recent advances have provided significant insight into the molecular and immunologic changes characteristic of SSc patients [1,2,3,4,5]; however, the underlying mechanisms that initiate and perpetuate disease pathologies remain poorly understood.
Evidence for dysbiosis as a source of disease pathology is well-documented in inflammatory skin conditions, such as psoriasis, where patients exhibit significant increases in both Propionibacterium and Staphylococcus on lesional skin . In atopic dermatitis (AD), patients exhibit temporal shifts in skin microbiome composition, with microbiome diversity decreasing during disease flares, characterized by significant increases in Staphylococcus levels, followed by increased diversity thereafter . These patterns of dysbiosis suggest a mechanism by which relative changes in the abundance of specific taxa directly influence disease pathology .
A wide array of potential etiologic agents have been proposed for SSc, including viruses, bacteria, and fungi. Viruses such as cytomegalovirus (CMV), parvovirus B19, Epstein-Barr virus (EBV), and endogenous retroviruses have all been postulated as potential triggers of SSc [8,9,10]. EBV transcripts have been reported in lesional skin of SSc patients . Among bacteria, Helicobacter pylori has been implicated in the etiology and progression of numerous autoimmune diseases, though its role in SSc remains controversial with studies both confirming and refuting such an association [10, 12, 13]. The most recent addition to the list of potential etiologic agents is the fungus Rhodotorula glutinis, a ubiquitous environmental contaminant and occasional skin commensal, which was found to be strongly associated with lesional skin of early-stage, untreated diffuse SSc patients .
Here, we examined skin biopsies from a diverse cohort of SSc patients and healthy controls by RNA-sequencing (RNA-seq) to obtain an unbiased assessment of the SSc microbiome and its relationship with patient gene expression. We show a reproducible shift in microbiome composition characterized by decreases in lipophilic taxa, along with increases in a variety of gram-negative bacteria that mirror local changes in inflammatory gene expression. These changes are closely tied to underlying gene expression associated with lipid signaling and immune activation. Genus-level taxonomic changes were associated with disease duration and the inflammatory intrinsic gene expression subset. Together, these data demonstrate that the skin microbiome composition in SSc mirrors molecular pathogenesis.
Study participants provided written, informed consent prior to sample collection in accordance with the Declaration of Helsinki Protocol and the Institutional Review Boards of Boston University Medical Center, Boston, MA, Dartmouth-Hitchcock Medical Center, Lebanon, NH, and the Hospital for Special Surgery, New York, NY. All patients met the American College of Rheumatology classification criteria for SSc , with further classification as either diffuse  (dSSc) or limited  (lSSc) disease. SSc patients with disease duration < 2.5 years were classified as “early stage” and patients with disease duration > 8 years were classified as “late stage” for this analysis.
Biopsy processing and RNA-seq
Lesional forearm and, for a subset of patients, non-lesional back skin was collected by punch biopsy (4 mm) from 15 SSc patients and 6 healthy volunteers. An additional 8 baseline samples collected as part of a Nilotinib clinical trial  were also included in this analysis. Following collection, samples were immediately placed in RNALater (Life Technologies, Carlsbad, CA) at 4 °C overnight, followed by − 80 °C until needed. Tissue homogenization was performed using the Qiagen TissueLyser II (Qiagen, Gaithersburg, MD). RNA extraction was performed using the Qiagen RNeasy Fibrous Tissue Mini Kit run on the QIAcube (Qiagen). RNA concentration and RNA integrity were assessed using the Agilent 4200 TapeStation (Agilent, Santa Clara, CA). RNA-seq libraries were generated from 100 ng total RNA prepared using the Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Gold rRNA depletion (Illumina, San Diego, CA). Samples were then multiplexed and sequenced on an Illumina NextSeq 500 sequencer, producing an average of 80–100 million 75-bp paired-end reads per sample.
Human gene expression analyses
Raw sequencing reads were aligned to the human genome (hg19) using STAR aligner  and expressed as fragments per million mapped reads (FPKM). Designation of intrinsic molecular subsets for SSc patients was performed using a gene-specific normalization method to render RNA-seq values distributions similar to microarray so that supervised machine learning algorithms can be applied regardless of the platform used to generate data, as described . Normalized RNA-seq data were classified using a support vector machine trained using a merged and curated dataset composed of samples from GSE9285, GSE32413, and GSE45485. To visualize results, the probe ID list from Johnson et al.  was collapsed on gene ID. This gene list was compared against normalized FPKM values for all 36 RNA-seq samples, resulting in a total of 1010 overlapping genes; a full list of all genes and normalized expression values is shown in Additional file 1: Table S1. Data were then hierarchically clustered using Cluster 3.0  and visualized using Java TreeView .
Metagenomic filtering and microbiome annotation
Metagenomic filtering and microbiome annotation was run using the Integrated Metagenomic Sequencing Analysis (IMSA) software package  and compared against the National Center for Biotechnology Information (NCBI) non-redundant nucleotide database (minimum significance = 1 × 10− 15), followed by a secondary BLAST alignment against the NCBI viral genome repository (minimum significance = 1 × 10− 5). To limit inclusion of spurious hits, sample annotation was limited to sequences mapping to five or fewer species, with ties split equally across species. Outputs were then filtered based on taxonomy to include only archaea, bacteria, fungi, and viruses. Normalization of taxonomic outputs was performed by rounding down to the nearest integer and rarefying to the level of the depth of the lowest sample using the Quantitative Insights Into Molecular Ecology (QIIME) platform . Batch effects associated with library preparation were removed by median centering across taxa. Statistical analyses were performed using Statistical Package for the Social Sciences (SPSS) software (IBM, version 23); additional analyses, including corrections for multiple hypothesis testing using the method of Benjamini & Hochberg , were performed in R.
Pathway activation and microbiome abundance
Single-sample gene set enrichment analysis (ssGSEA)  was run as a module in GenePattern, using relevant KEGG pathways as the query gene sets. A correlation matrix was then generated by calculating Pearson’s correlations for all combinations of ssGSEA values and genus-level abundance across all patients. Data were then hierarchically clustered using Cluster 3.0 and visualized using Java TreeView.
Lesional forearm skin biopsies were collected from 23 SSc patients; seven patients also provided biopsies of non-lesional back skin. Forearm skin biopsies were also obtained from 6 age- and gender-matched healthy controls. Samples were collected from three independent clinical centers and included both clinically limited (lSSc) and diffuse (dSSc) disease, with disease duration ranging from 0 to 35 years. The patient population consisted primarily of early-stage patients (disease duration ≤ 2 years), though a handful of very late-stage patients (disease duration > 10 years) were also included to assess microbiome changes over time. Clinical information on these patients is summarized in Table 1; a full breakdown of patient clinical information is presented in Additional file 2: Table S2. Assessments of skin involvement were determined based on overall modified Rodnan skin score (mRSS), as local scores were not available for all patients. No significant differences in age, sex, or race were evident between SSc and controls (p > 0.05 for all).
Sequencing and annotation
RNA-seq was performed on 36 skin biopsies, from 29 unique patients, resulting in an average of 83 million reads per sample (range 51,278,817–112,643,430). Raw sequencing reads were aligned to the human genome (hg19) using STAR aligner , and the expression level of each gene was expressed as fragments per million mapped reads (FPKM). Intrinsic gene expression subset designations were determined based on support vector machine classification using normalized FPKM values . Hierarchical clustering using the gene list from Johnson et al. , resulting in a total of 1010 overlapping genes, revealed distinct molecular subsets of disease, characterized by strong immune activation, lipid signaling, and proliferation signals, consistent with previous publications [1,2,3] (Fig. 1a; Additional file 1: Table S1). Together, these data suggest our patient cohort is representative of the four major intrinsic gene expression subsets of SSc. Additionally, we find that forearm and back samples largely tend to cluster together, consistent with previous analyses (Fig. 1b) .
Filtering of human sequence reads and microbiome annotation was performed using Integrated Metagenomic Sequence Analysis (IMSA) , yielding an average of 18,794 informative hits, defined as sequences mapping to five or fewer species, per skin biopsy (range 3098–74,429) across 1870 genera. To adjust for library-specific effects, all data were rarefied to the level of the lowest sample, followed by median centering of each genus by library preparation batch. This approach substantially reduced batch effects associated with library preparation, enabling direct comparisons of sample outputs across patients.
Antimicrobial gene expression is suppressed in SSc lesional skin
Antimicrobial peptides (AMPs), including cathelicidin (CAMP/LL-37), α-defensins, and β-defensins, are an essential component of epithelial barrier defenses. To assess the role of AMPs in SSc, we compared gene expression levels between SSc and controls, as well as between lesional forearm and non-lesional back skin. Among the major AMPs, dermcidin (DCD) is highly expressed across samples, regardless of disease type, while other major AMPs, including cathecidin (CAMP) and the α-defensins, were virtually undetected, with no difference in expression between SSc and controls. In contrast, β-defensin 1 (DEFB1), an AMP produced by epithelial cells, is expressed across all samples; however, these levels are significantly lower is SSc lesional skin compared to healthy controls (p < 0.001 by unpaired t-test), as well as in lesional forearm compared to non-lesional back skin (p = 0.007 by paired t-test) (Additional file 3: Table S3). Similar results were also seen between SSC lesional skin and healthy controls in a previous SSc skin RNA-seq dataset (Additional file 3: Table S3), suggesting a potential mechanism underlying microbiome differences in SSc patients.
Microbiome genus-level differences are correlated with SSc clinical phenotypes
SSc patients exhibited large changes in microbiome composition relative to controls, characterized by decreases in lipophilic taxa, such as Propionibacterium and Staphylococcus, combined with increases in a wide range of Gram-negative bacteria, including Burkholderia, Citrobacter, and Vibrio (p < 0.05 for all; Fig. 2a; Additional file 4: Table S4). These differences were not associated with clinical subtype, with limited and diffuse disease exhibiting broadly similar abundances of major taxa (Fig. 2a). Decreases were also observed in the fungus Malassezia relative to controls, with the greatest decrease occurring in dSSc patients.
Associations between disease duration and genus-level abundance were also evident, with significant (p < 0.05) or near-significant (p < 0.10) differences in 6 of the top 21 genera, including Propionibacterium, Salmonella, and Enterobacter (Fig. 2b; Additional file 4: Table S4). Relative decreases in Propionibacterium were evident for both early- and late-stage patients, relative to controls. We observed differential directions for the relative abundance of Salmonella including significant increases for early-stage patients and reduced abundance in late-stage patients. Comparisons between the four intrinsic molecular subsets of disease revealed modest differences associated with the normal-like and inflammatory subsets, with normal-like patients broadly mimicking differences seen between SSc and controls, while the inflammatory group was characterized by decreased Staphylococcus and increased Roseolovirus, relative to other subsets (Fig. 2c). The absence of more acute genus-level distinctions between subsets is likely the result of high levels of some genera in both inflammatory and proliferative patients (Fig. 2c), thereby limiting the diagnostic value of any single genus. Other clinical cofactors, including sex and autoantibody status, were not statistically different between groups. A full list of comparisons for each clinical cofactor is shown in Additional file 4: Table S4.
Core microbiome by patient is predictive of clinical involvement
To identify changes in microbiome composition associated with clinical covariates, we calculated the number of taxa that accounted for 90% of the annotated reads across our entire dataset, which we collectively refer to as the SSc skin core microbiome. The SSc skin core microbiome was composed of 103 genera and included representatives from bacteria, fungi, and viruses. Organisms not included in the core microbiome were exclusively low abundance taxa found in only a small number of samples. Hierarchical clustering of the SSc skin core microbiome revealed patterns of microbial abundance closely mimicking that seen within an individual, characterized by clear differences between SSc and controls (Fig. 3a). Organisms of the SSc skin microbiome formed distinct branches within the dendrogram. Lipophilic commensals (Malassezia, Propionibacterium, and Cutibacterium) were the predominant genera in normal-like patients, Gram-negative bacteria (Veillonella, Prevotella, Neisseria, and Actinomycetes) were abundant in the limited and proliferative subsets, and viruses (Roseolovirus and Cyprinivirus) were highest in inflammatory patients (Fig. 3a). These patterns are consistent with the various environmental niches associated with each class of organisms and are suggestive of changes in skin morphology and immune activation associated with each subset.
SSc skin microbiome profiles were analyzed using principal component analysis (PCA) to identify the broad, population-based changes associated with clinical covariates. Lesional forearm and non-lesional back skin were not significantly different among SSc patients (p = 0.097; Fig. 3b; Additional file 5: Figure S1). Similarly, no significant differences were evident based on SSc clinical subtype (p = 0.156; Fig. 3c) or mRSS at the time of biopsy (Additional file 6: Figure S2). In contrast, microbiome profiles were strongly correlated with intrinsic subset, with the strongest differences seen in normal-like and inflammatory patients, indicative of a link between disease activity of microbial abundance (p = 0.014; Fig. 3a, d).
Microbiome composition is correlated with inflammatory pathway activation in SSc skin biopsies
Given the close association seen between clinical subtype and microbiome composition, we next sought to identify relationships between relevant molecular pathways and taxonomic abundance using single-sample gene set enrichment analysis (ssGSEA). ssGSEA analysis generates a single value quantifying the extent to which a given gene set is coordinately up- or downregulated in a sample. This analysis was repeated for all available KEGG pathways, generating a table of pathway activation scores for each patient sample (Additional file 7: Table S5). Using this data, we then used Pearson’s correlations to compare each of these individual pathways against all genera in the SSc skin core microbiome (Additional file 8: Figure S3). The resulting correlation matrix allows for a direct comparison of gene expression and microbiome composition (Fig. 4a).
Hierarchical clustering of this dataset revealed strong associations between human gene expression and microbial abundance. Processes such as T cell, B cell, chemokine, and transforming growth factor beta (TGFβ) signaling in the absence of fatty acid signaling are strongly indicative of the inflammatory subset (Fig. 4a, cluster 1). Lower immune activation signals in combination with major fatty acid metabolism processes are commonly seen in the proliferative subset (Fig. 4a, cluster 2), while fatty acid signaling in the absence of immune activation is most commonly seen in normal-like patients (Fig. 4a, cluster 3).
Taxonomic abundance was strongly associated with the molecular processes of immune activation, lipid metabolism, cell proliferation, and Notch/Wnt signaling (Fig. 4a). Clustering of these processes was strongly correlated with differences in microbial abundance between SSc and controls, with statistically significant differences evident in 5 of 7 clusters (paired t-test, p < 0.05 for all; Fig. 4a, b). Among the most significant clusters was cluster 1, dominated by major lipophilic taxa, such as Malassezia and Propionibacterium, along with numerous Gram-positive Actinobacteria species (Additional file 9: Figure S4). These organisms were significantly more abundant in healthy controls (p < 0.001 for Actinobacteria and Propionibacterium by paired t-test) and exhibited strong positive correlations to lipid metabolism and cell proliferation KEGG pathways (Fig. 4a, b). In contrast, cluster 5 exhibits substantial increases in a wide range of Proteobacteria and other Gram-negative taxa in SSc patients (p < 0.001 by paired t-test) and is strongly correlated with KEGG immune activation pathways, including Toll-like receptor (TLR) and TGFβ signaling (Fig. 4a, d). Cluster 3 shows strong, positive correlations with immune activation, lipid metabolism, and Notch/Wnt signaling and is associated with statistically significant decreases in Bacteroidetes levels in SSc patients (p = 0.028 by paired t-test), combined with modest increases in Proteobacteria, relative to controls (p = 0.085 by paired t-test; Fig. 4a, e). These data demonstrate a strong association between underlying gene expression and the composition of the skin microbiome in SSc.
Recent studies have provided significant insight into the immunologic and gene expression-based changes characteristic of SSc patients; however, the underlying mechanisms that initiate and perpetuate disease pathologies remain poorly understood. Analyses of the skin  and gut  of SSc patients have revealed substantial changes in microbiome composition (dysbiosis), though the role of these organisms in disease pathology is not known. Here, we examined skin biopsies from a diverse cohort of SSc patients by RNA-seq, allowing for the unbiased metagenomic analysis of all potential pathogens, including bacteria, fungi, and viruses, as well as providing a platform from which to investigate the relationship between microbiome composition and underlying gene expression. Limitations of this study include the small number of samples and incomplete clinical data for some patients including a lack of local skin scores. The bimodal distribution of disease duration in our cohort is a confounding factor when assessing changes in microbiome composition over time.
Previous studies examining the composition of the bacterial microbiome in healthy individuals identified three basic environments, dry, moist, and sebaceous, which are reflected in the bacterial populations of these regions . Sebaceous regions, such as the face and back, harbored large proportions of Propionibacterium and Staphylococcus species, while dry regions, such as the forearm, show a shift towards lower levels of Propionibacterium in combination with higher percentages of Proteobacteria, Corynebacteria, and Flavobacteriales . When considered in this context, where the bacterial microbiome changes as a function of local lipid and moisture levels, the data presented here paint a picture of SSc as a more extreme version of this process, with disease-specific anatomical changes playing a major role in shaping microbiome composition. Lesional SSc forearm skin exhibited significant decreases in Propionibacterium and Staphylococcus levels relative to controls, along with increases in a wide range of Proteobacteria (Fig. 2), a continuation of the normal differences seen between sebaceous and dry regions of healthy controls. The underlying basis for these changes is also evident at the molecular level, with abundance of lipophilic taxa, such as Propionibacterium and Malassezia, strongly associated with fatty acid metabolism and lipid signaling pathways, while Proteobacteria were more elevated in patients with active immune signaling (Fig. 4). Microbiome profiles were not significantly different between lesional forearm and non-lesional back skin in this study, with paired samples clustering strongly based on patients, with minimal effect seen in terms of the anatomical site (Additional file 5: Figure S3). This observation further implicates the underlying gene expression as a major driver of microbiome composition, as disease-related changes in gene expression are consistent between SSc lesional forearm and non-lesional back, yet the microbiome profiles of these sites are strongly divergent in healthy controls.
From a mechanistic standpoint, changes in the SSc skin microbiome may be attributed to physical changes associated with fibrotic skin. Atrophy of both hair follicles and sebaceous glands is commonly seen in SSc patients , resulting in the loss of both an essential food source, as well as the physical niche where many of these species reside . This loss of skin appendages leads to a weakening of the acid mantle, and a loss of skin barrier function .
The strong association between increased Gram-negative taxa, particularly Proteobacteria, and immune activation shown here suggests a potential link between the skin microbiome and immune activation. Analysis of host-microbiome interactions, particularly with the host immune system, is necessary to determine the extent to which these organisms are capable of exacerbating and perpetuating the inflammatory responses in SSc skin.
In a preliminary study of the skin microbiome, increased levels of R. glutinis were detected on lesional skin of four untreated, early-stage patients, with only background levels seen in controls, suggesting a potential link between disease etiology and the skin microbiome . Unfortunately, a direct assessment regarding the etiologic nature of this organism was not possible here due to differences in the two patient cohorts, both in terms of prior treatment and disease duration. The majority of early-stage patients described here were not receiving immunosuppressive therapy at the time of biopsy, though all were receiving treatment for SSc-associated symptoms, including vascular symptoms and gastrointestinal reflux. In contrast, five of six late-stage patients (disease duration > 5 years) were untreated at the time of biopsy, consistent with the more quiescent nature of the disease in this population. Rhodotorula sequences were consistently detected in both SSc and controls, though these levels never rose above the background noise. Such an observation indicates that while Rhodotorula may be increased in very early disease, colonization does not persist over time.
Few viral pathogens were detected in our cohort, with no reads associated with EBV, parvovirus B19, or CMV identified in lesional skin. In contrast, we did consistently identify sequences associated with Roseolovirus, a genus which contains both human Herpesviruses (HHV) 6 and 7, which exhibited modest increases in inflammatory SSc patients. As EBV (HHV4), CMV (HHV5), and Roseolovirus are all members of the Herpesvirus family, detection of active viral transcription in inflammatory lesional skin does suggest a potential link between life-long latent viral infections and disease pathology, though further studies will be necessary to prove such an association.
The data presented here demonstrate a possible mechanistic link between SSc skin microbiome composition and disease pathology, with a loss of skin appendages and lipid signaling leading to decreases in lipophilic taxa, and a shift to a largely Gram-negative environment. Host-microbiome studies will be necessary to assess the extent to which the microbiome shapes SSc-associated gene expression and vice versa.
Diffuse systemic sclerosis
Fragments per million mapped reads
Integrated metagenomic sequencing analysis
Limited systemic sclerosis
National Center for Biotechnology Information
Quantitative Insights Into Molecular Ecology
Statistical Package for Social Sciences
Single-sample gene set enrichment analysis
Transforming growth factor beta
Milano A, Pendergrass SA, Sargent JL, George LK, McCalmont TH, Connolly MK, Whitfield ML. Molecular subsets in the gene expression signatures of scleroderma skin. PLoS One. 2008;3(7):e2696.
Pendergrass SA, Lemaire R, Francis IP, Mahoney JM, Lafyatis R, Whitfield ML. Intrinsic gene expression subsets of diffuse cutaneous systemic sclerosis are stable in serial skin biopsies. J Invest Dermatol. 2012;132(5):1363–73.
Hinchcliff M, Huang C-C, Wood TA, Mahoney JM, Martyanov V, Bhattacharyya S, Tamaki Z, Lee J, Carns M, Podlusky S. Molecular signatures in skin associated with clinical improvement during mycophenolate treatment in systemic sclerosis. J Invest Dermatol. 2013;133(8):1979–89.
Johnson M, Mahoney J, Taroni J, Sargent J, Marmarelis E, Wu M, Varga J, Hinchcliff M, Whitfield M. Experimentally-derived fibroblast gene signatures identify molecular pathways associated with distinct subsets of systemic sclerosis patients in three independent cohorts. PLoS One. 2015;10(1):e0114017.
Mahoney JM, Taroni J, Martyanov V, Wood TA, Greene CS, Pioli PA, Hinchcliff ME, Whitfield ML. Systems level analysis of systemic sclerosis shows a network of immune and profibrotic pathways connected with genetic polymorphisms. PLoS Comput Biol. 2015;11(1):e1004005.
Weyrich LS, Dixit S, Farrer AG, Cooper AJ. The skin microbiome: associations between altered microbial communities and disease. Australas J Dermatol. 2015;56(4):268–74.
Kong HH, Oh J, Deming C, Conlan S, Grice EA, Beatson MA, Nomicos E, Polley EC, Komarow HD, Murray PR. Temporal shifts in the skin microbiome associated with disease flares and treatment in children with atopic dermatitis. Genome Res. 2012;22(5):850–9.
Grossman C, Dovrish Z, Shoenfeld Y, Amital H. Do infections facilitate the emergence of systemic sclerosis? Autoimmun Rev. 2011;10(5):244–7.
Hamamdzic D, Kasman LM, LeRoy EC. The role of infectious agents in the pathogenesis of systemic sclerosis. Curr Opin Rheumatol. 2002;14(6):694–8.
Radic M, Kaliterna DM, Radic J. Helicobacter pylori infection and systemic sclerosis-is there a link? Joint Bone Spine. 2011;78(4):337–40.
Farina A, Cirone M, York M, Lenna S, Padilla C, McLaughlin S, Faggioni A, Lafyatis R, Trojanowska M, Farina GA. Epstein-Barr virus infection induces aberrant TLR activation pathway and fibroblast-myofibroblast conversion in scleroderma. J Invest Dermatol. 2014;134(4):954–64.
Csiki Z, Gal I, Sebesi J, Szegedi G. Raynaud syndrome and eradication of helicobacter pylori. Orv Hetil. 2000;141(52):2827–9.
Danese S, Zoli A, Cremonini F, Gasbarrini A. High prevalence of Helicobacter pylori type I virulent strains in patients with systemic sclerosis. J Rheumatol. 2000;27(6):1568–9.
Arron ST, Dimon MT, Li Z, Johnson ME, Wood TA, Feeney L, Angeles JG, Lafyatis R, Whitfield ML. High Rhodotorula sequences in skin transcriptome of patients with diffuse systemic sclerosis. J Invest Dermatol. 2014;134(8):2138–45.
Preliminary criteria for the classification of systemic sclerosis (scleroderma). Subcommittee for scleroderma criteria of the American Rheumatism Association Diagnostic and Therapeutic Criteria Committee. Arthritis Rheum. 1980;23(5):581–90.
LeRoy EC, Black C, Fleischmajer R, Jablonska S, Krieg T, Medsger T Jr, Rowell N, Wollheim F. Scleroderma (systemic sclerosis): classification, subsets and pathogenesis. J Rheumatol. 1988;15(2):202.
Mayes MD. Classification and epidemiology of scleroderma. Semin Cutan Med Surg. 1998;1(1):22–6.
Gordon JK, Martyanov V, Magro C, Wildman HF, Wood TA, Huang W-T, Crow MK, Whitfield ML, Spiera RF. Nilotinib (Tasigna™) in the treatment of early diffuse systemic sclerosis: an open-label, pilot clinical trial. Arthritis Res Ther. 2015;17(1):1.
Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR. STAR: ultrafast universal RNA-seq aligner. Bioinformatics. 2013;29(1):15–21.
Franks JM, Cai G, Whitfield ML. Feature specific quantile normalization enables cross-platform classification of molecular subtypes using gene expression data. Bioinformatics. 2018.
de Hoon MJ, Imoto S, Nolan J, Miyano S. Open source clustering software. Bioinformatics. 2004;20(9):1453–4.
Saldanha AJ. Java Treeview—extensible visualization of microarray data. Bioinformatics. 2004;20(17):3246–8.
Dimon MT, Wood HM, Rabbitts PH, Arron ST. IMSA: integrated metagenomic sequence analysis for identification of exogenous reads in a host genomic background. PLoS One. 2013;8(5):e64546.
Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI. QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 2010;7(5):335–6.
Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc Ser B Methodol. 1995:289–300.
Barbie DA, Tamayo P, Boehm JS, Kim SY, Moody SE, Dunn IF, Schinzel AC, Sandy P, Meylan E, Scholl C. Systematic RNA interference reveals that oncogenic KRAS-driven cancers require TBK1. Nature. 2009;462(7269):108–12.
Whitfield ML, Finlay DR, Murray JI, Troyanskaya OG, Chi JT, Pergamenschikov A, McCalmont TH, Brown PO, Botstein D, Connolly MK. Systemic and cell type-specific gene expression patterns in scleroderma skin. Proc Natl Acad Sci U S A. 2003;100(21):12319–24.
Volkmann ER, Chang YL, Barroso N, Furst DE, Clements PJ, Gorn AH, Roth BE, Conklin JL, Getzug T, Borneman J. Association of Systemic Sclerosis With a Unique Colonic Microbial Consortium. Arthritis Rheum. 2016;68(6):1483–92.
Grice EA, Kong HH, Conlan S, Deming CB, Davis J, Young AC, Bouffard GG, Blakesley RW, Murray PR, Green ED. Topographical and temporal diversity of the human skin microbiome. Science. 2009;324(5931):1190–2.
Calonje JE, Brenn T, Lazar AJ, McKee PH. Pathology of the skin: Elsevier Health Sciences; 2011.
Tanghetti EA. The role of inflammation in the pathology of acne. J Clin Aesthet Dermatol. 2013;6(9):27–35.
Schmid-Wendtner M-H, Korting HC. The pH of the skin surface and its impact on the barrier function. Skin Pharmacol Physiol. 2006;19(6):296–302.
This work was supported by CDMRP Grant W81XWH-14-1-0224 from the Department of Defense to MLW and SA and a grant from the Scleroderma Research Foundation (MLW).
Availability of data and materials
The full RNA-seq data from this study will be made freely available from the NCBI Short Read Archive (SRA).
Ethics approval and consent to participate
Study participants provided written, informed consent prior to sample collection in accordance with the Declaration of Helsinki Protocol and the Institutional Review Boards of Boston University Medical Center, Boston, MA, Dartmouth-Hitchcock Medical Center, Lebanon, NH, and the Hospital for Special Surgery, New York, NY.
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Table S1. Gene expression data. Normalized RNA-seq data were classified using a support vector machine trained using a merged and curated dataset composed of samples from GSE9285, GSE32413, and GSE45485. To visual results, the probe ID list from Johnson et al.  was collapsed on gene ID. This gene list was compared against normalized FPKM values for all 36 RNA-seq samples, resulting in a total of 1010 overlapping genes. Intrinsic subset assignments for individual genes are not possible based on the nature of gene expression in SSc patients. Each patient’s intrinsic subset assignment was determined based on the collective co-expression of all 1010 genes in this dataset, with both high and low expression of individual genes important for determining subset distinctions. Furthermore, both high and low expression of individual genes often extends across multiple intrinsic subsets. This inherently prevents providing subset-specific calls for individual genes. (XLSX 418 kb)
Table S2. Full clinical data for all patients included in this study. (XLSX 14 kb)
Table S3. Antimicrobial gene expression in lesional and control skin. (XLSX 36 kb)
Table S4. Differences in genus-level abundance by clinical covariate. Statistical analyses were performed comparing genus-level abundance between groups, presented as p values. Data were compared using the Mann-Whitney U test, corrected for multiple hypothesis testing using the method of Benjamini & Hochberg. Statistically significant differences (p < 0.05) are highlighted in yellow; differences significant to p < 0.10 are shown in pink. (XLSX 24 kb)
Figure S1. Principal component analysis of lesional forearm samples based on mRSS. Principal component analysis of core microbiome profiles based on mRSS. Data were limited to SSc lesional forearm samples only. Patients were divided into quartiles based on mRSS score at the time of biopsy (low, < 5; medium, 6–15; high, 16–30; very high, > 30). (PPTX 77 kb)
Figure S2. Principal component analysis of paired lesional forearm samples. Core microbiome profiles from seven paired forearm and back samples were analyzed by principal component analysis to assess the relationship between anatomical sites. Samples were color coded by A) anatomical site, and B) patient. (PPTX 91 kb)
Table S5. Single-sample gene set enrichment analysis in SSc patients. Single-sample gene set enrichment analysis (ssGSEA)  was run as a module in GenePattern, using relevant KEGG pathways as the query gene sets. Raw pathway enrichment scores are shown for each sample in our dataset. (XLSX 102 kb)
Figure S3. Comparing gene expression with taxonomic abundance. Single-sample gene set enrichment analysis (ssGSEA) provides a quantitative measurement, expressed as a single value, describing the extent to which a given gene set is coordinately up- or downregulated in a sample. A. To reduce the dimensionality of the data, the activation of a given KEGG pathway was assessed using ssGSEA, reducing a large set of functionally related genes to a single value for each patient. This process was repeated for all available KEGG pathways, generating a table of pathway activation scores for each patient sample (Additional file 2: Table S2). B. Pearson’s correlations were then used to compare each set of pathway activation scores against the relative abundance of each genus in the SSc skin core microbiome. C. This process is repeated for each combination of KEGG pathway and genus, producing a correlation matrix. D. Data are then clustered hierarchically and visualized to identify patterns of gene expression, and its relationship to microbial abundance. (PPTX 113 kb)
Figure S4. Differences in kingdom- and phylum-level abundance between groups. Kingdom- and phylum-level abundance is shown for all major gene expression clusters (Fig. 4a, b). Data are presented as log2 median-centered values for all forearm biopsies from SSc (blue) and controls (gray). (TIF 7050 kb)
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Johnson, M.E., Franks, J.M., Cai, G. et al. Microbiome dysbiosis is associated with disease duration and increased inflammatory gene expression in systemic sclerosis skin. Arthritis Res Ther 21, 49 (2019). https://doi.org/10.1186/s13075-019-1816-z
- Systemic sclerosis