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Mycophenolate mofetil for systemic sclerosis: drug exposure exhibits considerable inter-individual variation—a prospective, observational study

Abstract

Objective

Mycophenolate mofetil (MMF) is an established therapy for systemic sclerosis (SSc), but its pharmacokinetics in this disease remains unexplored. We have investigated drug exposure in MMF-treated patients with SSc in relation to clinical features of the disease and common concomitant drugs.

Methods

This study was predefined to include 35 MMF-treated SSc patients who were using MMF at a fixed dose of 0.5, 1.0 or 1.5 g twice daily since at least 3 months. The 12-h drug exposure of the active MMF metabolite mycophenolic acid (MPA) was estimated by repeated analysis of plasma MPA over a 6-h period. This 12-h drug exposure was dose normalised to a daily intake of 3 g MMF (MPA_AUC3g) in order to compare subjects using MMF at different doses. Drug exposure was analysed in reference to the clinical characteristics including body weight, renal function, autoantibodies, intestinal dysbiosis, intestinal inflammation assessed by faecal (F)-calprotectin, intestinal symptoms assessed by the University of California Los Angeles Scleroderma Trial Consortium Gastrointestinal Tract Instrument 2.0 and concomitant drug usage including proton-pump inhibitors (PPI).

Results

Thirty-four out of 35 study participants completed the study. The mean daily MMF dose was 2.1 g. Drug exposure expressed as MPA_AUC3g varied up to 8-fold between patients (median 115, range 27–226 mg h/L).

MPA_AUC3g was inversely related to body weight (rs = − 0.58, p < 0.001) and renal function (rs = − 0.34, p = 0.054). Anti-topoisomerase-1 antibodies and male sex were associated with lower MPA_AUC3g (87 vs 123 and 71 vs 141; p = 0.008 and p = 0.015, respectively). MPA_AUC3g was inversely related to the intestinal abundance of lactobacilli and to F-calprotectin (rs = − 0.54, p = 0.004; rs = − 0.36, p = 0.034), but not to gastrointestinal symptoms. MPA_AUC3g was inversely related to PPI usage (rs = − 0.45, p = 0.007). We found no association between MPA_AUC3g and disease subtype, disease duration or disease activity.

Conclusion

MMF-treated SSc patients exhibit considerable inter-individual variation in drug exposure, and lower MPA levels were primarily found in PPI users with poor prognostic factors. Body weight, renal function, sex, serology, gastrointestinal manifestations and/or measuring individual MPA exposure should be considered when using MMF for SSc.

Introduction

Mycophenolate mofetil (MMF) was introduced in 1995 as an immunosuppressant to prevent graft rejection in recipients of solid organ transplants [1]. Today, MMF is also an established therapy in systemic sclerosis (SSc) [2,3,4,5].

The pharmacokinetics of MMF is complex [6, 7]. Briefly, ingested MMF is hydrolised to the active metabolite mycophenolic acid (MPA) in the stomach and then rapidly absorbed. MPA is converted to the inactive metabolite MPA-7-O-glucoronide (MPAG) in the liver and kidney and excreted in the bile and urine. MPAG that reaches the intestine is deconjugated to MPA by anaerobe bacteria and readily reabsorbed [7].

Individual MPA exposure is difficult to estimate based on a single analysis of plasma MPA. A good estimation of MPA exposure can be made by measuring plasma MPA multiple times during a 3–6-h period and calculating MPA area under the concentration-time curve 0–12 h (MPA_AUC0–12) [6,7,8].

The treatment effect of MMF in recipients of solid organ transplants has been studied in relation to MPA_AUC0–12. Levels below 30 mg h/L have been associated with increased risk of irreversible transplant failure while exposure above 60 mg h/L did not improve outcomes, but caused more frequent side effects. Consequently, monitoring of MPA exposure has been advocated for recipients of solid organ transplants [6].

The pharmacokinetics of MMF has also been investigated in systemic lupus erythematosus (SLE) and chronic graft versus host disease (GvHD), where data suggests that drug efficacy is dependent on plasma concentration and not on dose [9, 10]. In SLE, the treatment effect levelled out when MPA exposure exceeded 45 mg h/L and a target MPA_AUC0–12 level of 35 mg h/L has been suggested for this disease [9]. In GvHD, a drug exposure of at least 30 mg h/L has been suggested [10].

Gastrointestinal involvement in SSc is multifaceted and has been associated with dysbiosis and malabsorption [11, 12]. Dysbiosis in SSc has been characterised by a relative abundance of lactobacilli in three independent studies [12,13,14]. Malnutrition and malabsorption have been associated with poor outcome in new-onset SSc and altered uptake of the drug penicillamine [15, 16].

In SSc, MMF at a target dose of 3 g daily has shown equivalent efficacy as cyclophosphamide against lung fibrosis [2]. Later studies have confirmed a role for MMF in the treatment of skin and lung fibrosis in SSc, albeit our knowledge on optimal dosing remains limited [3,4,5, 17]. Both the immunosuppressive and the antifibrotic effects of MPA are concentration-dependent [6, 18].

The purpose of this study was to investigate MPA exposure in SSc subjects treated with MMF. Our secondary objective was to investigate how the pharmacokinetics of MMF relates to clinical characteristics including gastrointestinal factors and to medications commonly used in SSc.

Patients and methods

Patients

This prospective open-label study encompassed patients with SSc fulfilling the 2013 ACR/EULAR classification criteria who were using MMF tablets in a fixed dose twice daily since ≥ 3 months [19]. Participants were recruited from all over Sweden with the help of the national patient organisation for SSc. Based on our current knowledge on the pharmacokinetics of MMF, patients with a history of gastrointestinal surgery including resection of any part of the gastrointestinal tract, renal failure or pulmonary arterial hypertension were excluded [7, 20]. Because of the necessity of multiple venepunctures, we excluded patients with a history of complicated venepunctures or anaemia. Further exclusion criteria included pregnancy, ongoing infection and previous solid organ transplantation.

Patient and public involvement

The study design was developed in collaboration with the national patient organisation for SSc.

Mycophenolate mofetil

Participants spent the night at the hospital or in direct adjunction to the hospital. They were instructed to follow their usual morning routine regarding concomitant intake of food and other medications together with MMF. Blood samples were drawn right before ingestion of the morning dose (which was administered under supervision) and 60, 180, and 360 min later. Plasma MPA levels were measured using high-performance liquid chromatography. MPA exposure was estimated based on a model previously developed for autoimmune diseases: MPA_AUC0–12 = 12.3 + 4.7 × C0 + 1.2 × C1 + 2.7 × C3 + 1.8 × C6 mg h/L [8].

Patient characteristics

Disease subtype, antibody status, disease duration since first non-Raynaud’s manifestation, disease activity and skin involvement were defined in accordance with the EUSTAR recommendations [21]. Renal function was estimated based on a combination of measurement of plasma creatinine and plasma cystatin C [22]. Gastrointestinal microbiota dysbiosis was analysed with the GA-map™ Dysbiosis Test (Genetic Analysis, Oslo, Norway) and the relative abundance of lactobacilli analysed [12]. Gastrointestinal inflammation was assessed by faecal (F)-calprotectin (Calpro AS, Norway), and levels above 50 μg/g were considered pathological [11]. Gastrointestinal symptoms were evaluated by the University of California Clinical Trial Consortium Gastrointestinal Tract Instrument (UCLA SCTC GIT) 2.0 [23]. Disease activity was assessed by the EULAR revised activity index [24] and malnutrition risk by the Malnutrition Universal Screening Tool (MUST) [25]. Participants were asked to report recent intake of proton-pump inhibitors (PPI), calcium channel blockers and NSAID by filling out designated forms.

Statistical analyses

The study was predefined to include thirty-five patients, a number that was chosen together with a senior statistician to be able to do subgroup analyses [6]. In order to compare the relative drug exposure in patients prescribed different doses of MMF, we constructed the variable MPA_AUC3g corresponding to a daily intake of 3 g MMF. This estimate was made by multiplying MPA_AUC0–12 by 3 or 1.5, respectively, in patients using MMF at a dose of 1 or 2 g daily [26, 27]. Descriptive data were presented using mean ± (SD) or median (IQR or range), and comparative analyses were done using non-parametric statistics including Spearman’s rank correlation (rs) coefficient, Kruskal-Wallis test and the Mann-Whitney U test.

Based on our current knowledge on the pharmacokinetics of MMF, we studied MPA exposure in relation to weight, renal function and concomitant medications [6, 28].

Based on previous reports on malabsorption in SSc, we also set out to explore MPA exposure in relation to gastrointestinal symptoms and inflammation, the MUST and intestinal microbiota [11,12,13,14,15,16, 23, 25].

Based on current knowledge on SSc prognosis for specific SSc subset, we also set out to explore MPA exposure in relation to skin involvement and serological profile [29].

Ethics

This study was approved by the Swedish Ethical Review Authority (Dnr 2018/490) and the Swedish Medical Products Agency (EudraCT 2018-002105-54) and prospectively registered at ClinicalTrials.gov (NCT03678987, posted September 20, 2018). All participants gave their written informed consent prior to entering the study. The study was conducted in accordance with the Declaration of Helsinki.

Results

Thirty-four out of predefined 35 study participants completed the study. Patient characteristics are presented in Table 1 and show some heterogeneity with regard to the MMF dose. Most notably, renal function that was lower in patients using MMF at a lower dose.

Table 1 Patient characteristics. Data are shown as numbers and per cent (%) or means ± standard deviation (SD) or median interquartile range (IQR)/range and in relation to daily dose MMF

The mean daily MMF dose was 2.1 g. MPA exposure exhibited considerable variation between patients (Table 2). Two subjects exhibited an estimated MPA_AUC0–12 < 30 mg h/L while 25 subjects exhibited an estimated MPA_AUC0–12 > 60 mg h/L. The MPA_AUC0–12 displayed a linear dose-dependent relationship with MMF intake (Table 2), and MPA_AUC3g was therefore used for further analyses.

Table 2 MPA exposure in MMF-treated systemic sclerosis. The mean and median MPA exposure correlated to MMF intake in a dose-dependent manner. There was a considerable inter-individual variation in MPA exposure in all three subgroups

MPA_AUC3g varied inversely with body weight (rs = − 0.58, p < 0.001) and the estimated glomerular filtration rate (rs = − 0.34, p = 0.054). Male subjects had lower MPA_AUC3g levels than their female counterparts (74 vs 121 mg h/L; p = 0.015; Supplemental Figure 1). Of note, the correlation between MPA_AUC3g and renal function and sex could not be corroborated when relating these variables to MPA exposure expressed as MPA_AUC0–12 (Table 3).

Table 3 Associations between MPA exposure and clinical characteristics

MPA exposure exhibited significant heterogeneity in relation to four serological groups (Table 3, p value for between-group differences = 0.005). Patients with anti-topoisomerase-1 autoantibodies had lower MPA_AUC3g compared to other participants (median 87 vs 123 mg h/L; p = 0.008; Table 3, Fig. 1).

Fig. 1
figure 1

MPA exposure in relation to daily MMF intake. MPA exposure, defined by the variable MPA_AUC0–12 varied considerably between patients. Patients with anti-topoisomerase-1 antibodies had significantly lower drug exposure compared to the other subjects

Dysbiosis was present in 14 of the 27 patients tested and was not associated with altered MPA exposure (Table 3). However, there was a negative association between the relative prevalence of lactobacilli and MPA_AUC3g (rs = 0.54, p = 0.004; Fig. 2). Patients with normal F-calprotectin had higher MPA_AUC3g compared to patients with pathological F-calprotectin levels (127 vs 99 mg h/L, p = 0.040), and F-calprotectin levels correlated inversely with MPA_AUC3g (rs = − 0.36, p = 0.025). We were unable to find an association between MPA exposure and gastrointestinal symptoms or malnutrition assessed by the UCLA SCTC GIT 2.0 and MUST (Table 3). However, MPA_AUC3g was negatively associated with PPI usage (rs = − 0.45, p = 0.007; Supplemental Figure 1).

Fig. 2
figure 2

MPA exposure in relation to intestinal lactobacilli levels. Patients with increased levels of lactobacilli in their stool had lower MPA exposure

We were not able to find any association between MPA exposure and calcium channel blockers or NSAID usage, nor disease duration, diffuse cutaneous disease subtype or disease activity (Table 3).

All five patients prescribed MMF at a dose of 1 g daily had previously used the drug at a higher dose. Of these, three had experienced suspect adverse events resulting in lowering of the dose while two patients were prescribed the lower dose of 1 g twice daily when their disease was considered stable. In total, five patients reported adverse events associated with MMF. A history of adverse events was not associated with MPA_ AUC3g (p = 0.508).

Discussion

MMF is an immunosuppressive and anti-fibrotic drug that shows a plasma concentration-dependent efficacy when used for solid organ transplantation, SLE and GvHD [1, 9, 10]. It is an established therapy in SSc where available data support its use at a target dose of 3 g daily [2]. We report considerable inter-individual variation in MPA exposure following oral MMF treatment in SSc. We also report that 25 out of 34 of the study participants exhibited MPA_AUC0–12 above the recommended interval for recipients of solid organ transplantation (30–60 mg h/L) [6], even though the average daily dose was only 2.1 g. These results need to be validated but indicate that it is common that SSc patients treated according to the current guidelines are exposed to higher levels of MPA than has been recommended for other diseases. One should be careful not to extrapolate safety data from previous MMF studies, to SSc patients prescribed MMF at a daily dose of 2 or 3 g.

In this study, we found that anti-topoisomerase-1 positivity and male sex predicted lower level of MPA exposure. We also identified that both an SSc-specific alteration in the microbiota and gastrointestinal inflammation were associated with lower levels of MPA exposure, suggesting that gastrointestinal manifestations of SSc may alter the bioavailability of MPA. We do not know what drug exposure is optimal for SSc. Our findings could be of clinical importance for newly diagnosed anti-topoisomerase-1 positive male patients with malabsorption. These patients have a poor prognosis, partly because of progressive lung fibrosis, and are in need of the full therapeutic potential of MMF [15, 29].

We found that PPI intake was associated with lower MPA exposure which is in line with previous reports. The mechanism behind this finding has been suggested to be explained the pH-dependent hydrolisation of MMF to MPA in the stomach [30]. This finding is of clinical significance considering the frequency by which PPIs are used for SSc [5].

Patients with the highest drug exposure had poor renal function and/or low body weight. This finding is in line with previous studies in other diseases and questions the rationale of a fixed target dose for all SSc patients.

The limitations of this study include the fact that we estimated MPA exposure using a 4-point model that has been designed for autoimmune diseases but not validated for SSc or for MPA measurement using high-performance liquid chromatography [8]. This study is limited to 34 patients which must be taken into consideration when extrapolating data from our subgroup analyses. Also, this study includes patients using MMF at three different daily doses with some heterogeneity between the groups, e.g. renal function, age, weight, disease duration and years on MMF (Table 1). Furthermore, we have not investigated the impact of MPA exposure on drug toxicity and drug efficacy. This issue needs further exploration before we can recommend therapeutic drug monitoring in MMF-treated SSc on a routine basis. Finally, because some of the patients that were excluded from the study were likely to suffer from a more severe disease (e.g. patients with a history of complicated venepunctures, renal failure or anaemia), the results may not be representative for the whole SSc population. There may also exist a selection bias towards participants who were able to tolerate this medication.

The optimal therapeutic interval for MPA in SSc remains to be defined. Considering the multiple modes of action by which this drug may modulate autoimmune fibrotic disease [31], it is possible that higher plasma concentrations compared to other diseases are needed in order for the drug to exhibit its full effect in SSc. In vitro studies have shown that not only the immunosuppressive function but also the anti-fibrotic effect of MPA is concentration-dependent [18]. Future studies are needed to investigate the clinical outcome of MMF therapy in SSc in relation to MPA_AUC0–12, as has been done in SLE [9]. We therefore advocate MPA exposure to be analysed in clinical SSc trials as was alluded to already by the authors of the SLS-II trial: “while serum levels of MMF [sic] might have provided some insight, they were not obtained” [2].

Individual therapeutic monitoring of MMF together with an appropriate dose adjustment has successfully been used for recipients of solid organ transplants because of the concentration-dependent efficacy of this drug [6]. Based on these experiences, measurement of plasma MPA and estimation of MPA_AUC0–12 are currently available in many clinics worldwide.

In the era of personalised medicine, we suggest that individual MPA exposure and its relation to body weight, renal function, prognostic markers and PPI usage should be considered when using MMF in SSc.

Conclusions

MMF-treated SSc patients exhibit considerable inter-individual variation in drug exposure. Higher levels are found in female patients with poor renal function and low body weight. Relatively lower levels are found in overweight PPI users with poor prognostic factors. Body weight, renal function, sex, serology, gastrointestinal manifestations and the possibility of individual analysis of MPA levels should be considered when using and evaluating MMF for SSc.

Availability of data and materials

The dataset supporting the conclusions of this article is available upon reasonable request.

Abbreviations

ACR:

American College of Rheumatology

EudraCT:

European Union Drug Regulating Authorities Clinical Trials Database

EULAR:

European League Against Rheumatism

EUSTAR:

The European Scleroderma Trials and Research group

GvHD:

Graft versus host disease

MMF:

Mycophenolate mofetil

MPA:

mycophenolic acid

MPA_AUC0–12 :

MPA area under the concentration-time curve 0–12 h

MPA_AUC3g :

MPA_AUC0–12 adjusted to a daily intake of 1.5 g MMF twice daily

MPAG:

MPA-7-O-glucoronide

MUST:

Malnutrition Universal Screening Tool

PPI:

Proton-pump inhibitor

SLE:

Systemic lupus erythematosus

SSc:

Systemic sclerosis

UCLA SCTC GIT:

University of California Clinical Trial Consortium Gastrointestinal Tract Instrument

References

  1. European Mycophenolate Mofetil Cooperative Study Group. Placebo-controlled study of mycophenolate mofetil combined with cyclosporine and corticosteroids for prevention of acute rejection. European Mycophenolate Mofetil Cooperative Study Group. Lancet. 1995;345(8961):1321–5.

    Article  Google Scholar 

  2. Tashkin DP, Roth MD, Clements PJ, Furst DE, Khanna D, Kleerup EC, et al. Mycophenolate mofetil versus oral cyclophosphamide in scleroderma-related interstitial lung disease (SLS II): a randomised controlled, double-blind, parallel group trial. Lancet Respir Med. 2016;4(9):708–19.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Volkmann ER, Tashkin DP, Li N, Roth MD, Khanna D, Hoffmann-Vold AM, et al. Mycophenolate mofetil versus placebo for systemic sclerosis-related interstitial lung disease: an analysis of Scleroderma Lung Studies I and II. Arthritis Rheumatol. 2017;69(7):1451–60.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Herrick AL, Pan X, Peytrignet S, Lunt M, Hesselstrand R, Mouthon L, et al. Treatment outcome in early diffuse cutaneous systemic sclerosis: the European Scleroderma Observational Study (ESOS). Ann Rheum Dis. 2017;76(7):1207–18.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Fernández-Codina A, Walker KM, Pope JE, Scleroderma Algorithm Group. Treatment algorithms for systemic sclerosis according to experts. Arthritis Rheumatol. 2018;70(11):1820–8.

    Article  PubMed  Google Scholar 

  6. van Gelder T, Le Meur Y, Shaw LM, Oellerich M, DeNofrio D, Holt C, et al. Therapeutic drug monitoring of mycophenolate mofetil in transplantation. Ther Drug Monit. 2006;28(2):145–54.

    Article  PubMed  Google Scholar 

  7. Benjanuwattra J, Pruksakorn D, Koonrungsesomboon N. Mycophenolic acid and its pharmacokinetic drug-drug interactions in humans: review of the evidence and clinical implications. J Clin Pharmacol. 2020;60(3):295–311.

    Article  CAS  PubMed  Google Scholar 

  8. de Winter BC, Neumann I, van Hest RM, van Gelder T, Mathot RA. Limited sampling strategies for therapeutic drug monitoring of mycophenolate mofetil therapy in patients with autoimmune disease. Ther Drug Monit. 2009;31(3):382–90.

    Article  PubMed  Google Scholar 

  9. Zahr N, Arnaud L, Marquet P, Haroche J, Costedoat-Chalumeau N, Hulot JS. Mycophenolic acid area under the curve correlates with disease activity in lupus patients treated with mycophenolate mofetil. Arthritis Rheum. 2010;62(7):2047–54.

    CAS  PubMed  Google Scholar 

  10. Wakahashi K, Yamamori M, Minagawa K, Ishii S, Nishikawa S, Shimoyama M, et al. Pharmacokinetics-based optimal dose prediction of donor source-dependent response to mycophenolate mofetil in unrelated hematopoietic cell transplantation. Int J Hematol. 2011;94(2):193–202.

    Article  CAS  PubMed  Google Scholar 

  11. Andréasson K, Scheja A, Saxne T, Ohlsson B, Hesselstrand R. Faecal calprotectin: a biomarker of gastrointestinal disease in systemic sclerosis. J Intern Med. 2011;270(1):50–7.

    Article  PubMed  Google Scholar 

  12. Andréasson K, Alrawi Z, Persson A, Jönsson G, Marsal J. Intestinal dysbiosis is common in systemic sclerosis and associated with gastrointestinal and extraintestinal features of disease. Arthritis Res Ther. 2016;18(1):278.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Volkmann ER, Chang YL, Barroso N, Furst DE, Clements PJ, Gorn AH, et al. Association of systemic sclerosis with a unique colonic microbial consortium. Arthritis Rheumatol. 2016;68(6):1483–92.

    Article  PubMed  PubMed Central  Google Scholar 

  14. Patrone V, Puglisi E, Cardinali M, Schnitzler TS, Svegliati S, Festa A, et al. Gut microbiota profile in systemic sclerosis patients with and without clinical evidence of gastrointestinal involvement. Sci Rep. 2017;7(1):14874.

    Article  PubMed  PubMed Central  Google Scholar 

  15. Richard N, Hudson M, Wang M, Gyger G, Proudman S, Stevens W, et al. Severe gastrointestinal disease in very early systemic sclerosis is associated with early mortality. Rheumatology (Oxford). 2019;58(4):636–44.

    Article  Google Scholar 

  16. Ammitzbøll T, Hendel L, Kreuzig F, Asboe-Hansen G. Bioavailability of D-penicillamine in relation to gastrointestinal involvement of generalized scleroderma. Scand J Rheumatol. 1987;16(2):121–6.

    Article  PubMed  Google Scholar 

  17. Mendoza FA, Lee-Ching C, Jimenez SA. Recurrence of progressive skin involvement following discontinuation or dose reduction of mycophenolate mofetil treatment in patients with diffuse systemic sclerosis. Semin Arthritis Rheum. 2020;50(1):135–9.

    Article  CAS  PubMed  Google Scholar 

  18. Roos N, Poulalhon N, Farge D, Madelaine I, Mauviel A, Verrecchia F. In vitro evidence for a direct antifibrotic role of the immunosuppressive drug mycophenolate mofetil. J Pharmacol Exp Ther. 2007;321(2):583–9.

    Article  CAS  PubMed  Google Scholar 

  19. van den Hoogen F, Khanna D, Fransen J, Johnson SR, Baron M, Tyndall A, et al. 2013 classification criteria for systemic sclerosis: an American College of Rheumatology/European League against Rheumatism collaborative initiative. Arthritis Rheum. 2013;65(11):2737–47.

    Article  PubMed  PubMed Central  Google Scholar 

  20. Sandqvist AM, Henrohn D, Schneede J, Hedeland M, Egeröd HC, Bondesson UG, et al. High inter-individual variability of vardenafil pharmacokinetics in patients with pulmonary hypertension. Eur J Clin Pharmacol. 2013;69(2):197–207.

    Article  CAS  PubMed  Google Scholar 

  21. Walker UA, Tyndall A, Czirják L, Farge-Bancel D, Kowal-Bielecka O, Müller-Ladner U, et al. Clinical risk assessment of organ manifestations in systemic sclerosis: a report from the EULAR Scleroderma Trials And Research group database. Ann Rheum Dis. 2007;66(6):754–63.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Grubb A. Non-invasive estimation of glomerular filtration rate (GFR). The Lund model: simultaneous use of cystatin C- and creatinine-based GFR-prediction equations, clinical data and an internal quality check. Scand J Clin Lab Invest. 2010;70(2):65–70.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Khanna D, Hays RD, Maranian P, Seibold JR, Impens A, Mayes MD, et al. Reliability and validity of the University of California, Los Angeles Scleroderma Clinical Trial Consortium Gastrointestinal Tract Instrument. Arthritis Rheum. 2009;61(9):1257–63.

    Article  PubMed  PubMed Central  Google Scholar 

  24. Valentini G, Iudici M, Walker UA, Jaeger VK, Baron M, Carreira P, et al. The European Scleroderma Trials and Research group (EUSTAR) task force for the development of revised activity criteria for systemic sclerosis: derivation and validation of a preliminarily revised EUSTAR activity index. Ann Rheum Dis. 2017;76(1):270–6.

    Article  CAS  PubMed  Google Scholar 

  25. Baron M, Hudson M, Steele R, Canadian Scleroderma Research Group. Malnutrition is common in systemic sclerosis: results from the Canadian Scleroderma Research Group database. J Rheumatol. 2009;36(12):2737–43.

    Article  PubMed  Google Scholar 

  26. Luszczyńska P, Pawiński T, Kunicki PK, Durlik M, Augustyniak-Bartosik H, Hurkacz M. Pharmacokinetics of free and total mycophenolic acid in adult lupus nephritis patients-implications for therapeutic drug monitoring. Eur J Clin Pharmacol. 2019;75(3):371–9.

    Article  PubMed  Google Scholar 

  27. Chaigne B, Gatault P, Darrouzain F, Barbet C, Degenne D, François M, et al. Mycophenolate mofetil in patients with anti-neutrophil cytoplasmic antibody-associated vasculitis: a prospective pharmacokinetics and clinical study. Clin Exp Immunol. 2014;176(2):172–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Veličković-Radovanović RM, Janković SM, Milovanović JR, Catić-Dordević AK, Spasić AS, Stefanović NZ, et al. Variability of mycophenolic acid elimination in the renal transplant recipients – population pharmacokinetic approach. Ren Fail. 2015;37(4):652–8.

    Article  PubMed  Google Scholar 

  29. Nihtyanova SI, Schreiber BE, Ong VH, Rosenberg D, Moinzadeh P, Coghlan JG, et al. Prediction of pulmonary complications and long-term survival in systemic sclerosis. Arthritis Rheumatol. 2014;66(6):1625–35.

    Article  PubMed  Google Scholar 

  30. Schaier M, Scholl C, Scharpf D, Hug F, Bönisch-Schmidt S, Dikow R, et al. Proton pump inhibitors interfere with the immunosuppressive potency of mycophenolate mofetil. Rheumatology (Oxford). 2010;49(11):2061–7.

    Article  CAS  Google Scholar 

  31. Morath C, Schwenger V, Beimler J, Mehrabi A, Schmidt J, Zeier M, et al. Antifibrotic actions of mycophenolic acid. Clin Transpl. 2006;20(Suppl 17):25–9.

    Article  Google Scholar 

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Acknowledgements

We acknowledge the skillful assistance of Ann-Christin Mårtensson, Clinical Chemistry, Skåne University Hospital, Malmö, and Arne Ståhl, Sahlgrenska University Hospital, Gothenburg.

We appreciate the cooperation with Riksföreningen för Systemisk Skleros in planning and conducting this study.

We are thankful to Professor Tore Saxne and Professor Frank Wollheim for the thoughtful advice in the planning of the study and interpretation of the results.

Funding

This study was funded by the Anna-Greta Crafoord Foundation, the Swedish Medical Society and the Swedish Rheumatism Association and Stiftelsen Ulla och Roland Gustafssons Donationsfond. Open Access funding provided by Lund University.

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Authors

Contributions

KA and RH planned the study and wrote the manuscript. KA and KN conducted the study and interpreted the results together with DW, DH, JM and RH. KN, DH and DW participated in the planning of the study. KN, DW, DH and JM reviewed the manuscript for intellectual content. The authors read and approved the final manuscript.

Corresponding author

Correspondence to Kristofer Andréasson.

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Ethics approval and consent to participate

This study was approved by the Swedish Ethical Review Authority (Dnr 2018/490) and the Swedish Medical Products Agency (EudraCT 2018-002105-54) and prospectively registered at ClinicalTrials.gov (NCT03678987, posted September 20, 2018). All participants gave their written informed consent prior to entering the study. The study was conducted in accordance with the Declaration of Helsinki.

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Not applicable.

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All authors declare no competing interests.

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Supplementary information

Additional file 1:

Supplemental Figure 1A-E. Scatter plot of MPA_AUC3g in relation to sex, body weight, renal function, relative omeprazole dose and F-calprotectin.

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Andréasson, K., Neringer, K., Wuttge, D.M. et al. Mycophenolate mofetil for systemic sclerosis: drug exposure exhibits considerable inter-individual variation—a prospective, observational study. Arthritis Res Ther 22, 230 (2020). https://doi.org/10.1186/s13075-020-02323-8

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