Total metabolic lesion volume of lymph nodes measured by 18F-FDG PET/CT: a new predictor of macrophage activation syndrome in adult-onset Still’s disease
Arthritis Research & Therapy volume 23, Article number: 97 (2021)
To investigate the potential utility of quantitative parameters obtained by 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) in the assessment of disease severity and the occurrence of macrophage activation syndrome (MAS) in adult-onset Still’s disease (AOSD).
Fifty-seven patients with AOSD who underwent pre-treatment 18F-FDG PET/CT were recruited in this study and compared with 60 age- and sex-matched healthy controls. Clinical features and laboratory data were recorded. The systemic score was assessed to determine the disease severity. The maximal standardized uptake value (SUVmax), metabolic lesion volume (MLV), and total lesion glycolysis (TLG) were used to evaluate the involved organs and tissues that abnormally accumulated 18F-FDG. Multivariate analysis was performed to identify the PET/CT-derived risk factors contributing to the AOSD-related MAS, and their diagnostic efficiency was evaluated.
High 18F-FDG accumulation was observed in the bone marrow (SUVmax median, 5.10), spleen (SUVmax median, 3.70), and lymph nodes (LNs, SUVmax median, 5.55). The SUVmax of the bone marrow (rho = 0.376, p = 0.004), SUVmax of the spleen (rho = 0.450, p < 0.001), TLGtotal of LNs (rho = 0.386, p = 0.017), and MLVtotal of LNs (rho = 0.391, p = 0.015) were correlated with the systemic score. The SUVmax of the spleen (p = 0.017), TLGtotal of LNs (p = 0.045), and MLVtotal of LNs (p = 0.012) were higher in patients with MAS than in those without MAS. A MLVtotal of LNs > 62.2 (OR 27.375, p = 0.042) was an independent predictive factor for MAS with a sensitivity of 80.0% and a specificity of 93.9%.
The glucose metabolic level of the spleen could be an effective and easy-to-use imaging indicator of disease severity, and MLVtotal of LNs > 62.2 was a strong predictor of MAS occurrence in patients with AOSD.
Adult-onset Still’s disease (AOSD) is a systemic inflammatory disease characterized by spiking fever, salmon-pink evanescent rash, arthralgia, and hepatosplenomegaly . The laboratory profile of AOSD is a reflection of systemic inflammation, including increased erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP), as well as hyperferritinemia, leukocytosis, and elevated levels of cytokines . Diagnosis of AOSD is based on clinical pattern recognition and exclusion of other autoimmune, inflammatory, infectious, or neoplastic diseases [3,4,5]. The patients with AOSD may experience life-threatening complications, especially companied with macrophage activation syndrome (MAS), with high mortality rate ranging from 10 to 42.5% [6, 7]. Early recognition and treatment of MAS is crucial but challenging, due to the lack of specific markers [8, 9].
18F-fluorodesoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT), which reflects both the glucose metabolic activity and the anatomical structure of target tissues, is commonly used on cancer diagnosis, staging, and prognosis, assessment of treatment response . For AOSD, 18F-FDG PET/CT has been used as a whole-body imaging tool to exclude malignancies and identify the biopsy sites when the patients were at admission [11, 12]. Previous studies (13 cases , seven cases , and 26 cases ) have revealed the significant increase of FDG uptake of involved organs in patients with AOSD. However, the correlations between abnormal glucose metabolism of involved organs, as determined by the maximal standardized uptake value (SUVmax), and disease severity are still illusive.
PET/CT-derived quantitative parameters based on three-dimensional volume of interest (VOI), namely metabolic tumor volume (MTV) and total lesion glycolysis (TLG), have been widely used in the assessment of whole-body disease burden and prognosis of tumors [15, 16]. Interestingly, recent studies presented associations between TLG and systemic inflammation in patients with recurrent colorectal cancer  and IgG4-related disease . Here, we quantified 18F-FDG uptake in patients with AOSD using SUV, MTV, and TLG, in pursuit of identifying markers associated with disease severity and AOSD-related MAS as well.
Material and methods
From November 2015 to January 2019, we identified 57 hospitalized patients diagnosed with AOSD according to the classification criteria proposed by Yamaguchi et al. at an academic hospital . Clinical manifestations and laboratory data were obtained on admission, and a systemic score, as proposed by Pouchot , was assessed for each patient. Macrophage activation syndrome (MAS) was diagnosed based on HLH-2004 criteria . All patients underwent 18F-FDG PET/CT scan for excluding malignancy before treatments of AOSD. In addition, to determine the glucose metabolic level of normal tissues and organs on PET/CT, 60 age- and sex-matched healthy controls without a history of autoimmune disease, recent infection, or malignant tumor were retrospectively recruited. The study was approved by the Ethics Committee of Ruijin Hospital, Shanghai Jiao Tong University School of Medicine.
18F-FDG PET/CT scanning was performed using the Discovery VCT64 system (GE Healthcare, Chicago, IL, USA). To control the blood glucose concentration below 7.4 mmol/L before scanning, patients were instructed to fast for at least 6 h. Patients received an intravenous injection of 5–6 MBq of 18F-FDG per kilogram of body weight 45–60 min before scanning from the skull base to the mid-thigh. PET images were acquired for 3 min per bed position using a matrix size of 128 × 128, 28 subsets, two iterations, and full-width half-maximum post-filtering. CT images were acquired using a tube voltage of 140 kV, a tube current of 220 mA, and a section thickness of 3.75 mm. The reconstruction of PET images was based on an ordered-subset expectation maximization algorithm with photon attenuation correction using CT data.
PET/CT image interpretation
Integrated PET and CT images were independently interpreted by two nuclear medicine physicians on the Advantage Workstation 4.4 system (GE Healthcare). The glucose metabolic level of the bone marrow, spleen, liver, and lymph nodes (LNs) in all patients with AOSD were assessed by the SUVmax and SUVmean within the VOI drawn on the PET/CT images. The abnormal hypermetabolism of these organs and tissues were defined as SUVmax higher than the upper limit of the 95% confidence interval for the glucose metabolic level of them in the healthy controls. LNs with 18F-FDG uptake lower than blood pool were excluded from analyses. In this study, the MTV was renamed as the metabolic lesion volume (MLV) because AOSD-affected lesions were non-neoplastic. Both the MLV and TLG  were measured on all hypermetabolic LNs. The MLV was automatically delineated using a threshold of 40% of the SUVmax. The TLG was defined as the MLV multiplied by the SUVmean. The total MLV (MLVtotal), which summed MTVs, and the total TLG (TLGtotal), which summed TLGs, in all hypermetabolic LNs were measured as the quantitative parameters of whole-body disease burden. Because of the irregular shape of the bone marrow and spleen, and the vicinity of the physiologically 18F-FDG-avid kidney, it was difficult to obtain VOIs completely covering either organ where the MLV and TLG could not be measured.
Statistical analysis was performed using SPSS (version 23, IBM Corporation) and R (version 3.6.2) software packages. Chi-square and Fisher’s exact tests were used to compare categorical variables. The Mann-Whitney rank sum test was used to compare continuous variables with non-normal distribution after the Kolmogorov-Smirnov test. The Wilcoxon signed-rank test was used to compare paired continuous variables with non-normal distribution. Spearman’s correlations were analyzed to examine the relationship between PET/CT parameters and laboratory indices with a Bonferroni correction. Binary logistic regression and Firth logistic regression  were used to evaluate the contribution of different clinical risk factors to disease severity. All the variables were included in the regression mode. The variables with p < 0.1 in univariate analysis were entered in multivariate analysis. Multicollinearity was excluded from the final model. Receiver operating characteristic curves (ROCs) were performed to determine the diagnostic performance of the PET/CT parameters. The optimum cutoff value was defined based on the maximum Youden index. The diagnostic accuracy of each parameter was reported together with its 95% confidence interval (CI). Statistical significance was defined as p < 0.05.
Clinical information of patients with AOSD
All the information is presented in Table 1. The median age of the 57 patients was 34, with a female predominance of 84.21%. The most prevalent clinical manifestations were arthralgia (91.23%) and skin rash (91.23%), followed by fever higher than 39 °C (80.70%). Increased levels of CRP (94.74%), serum ferritin (87.72%), and ESR (73.68%) were commonly observed. The median systemic score was 7.
Characterization of abnormal 18F-FDG accumulation in patients with AOSD
The accumulation of 18F-FDG was significantly higher in the bone marrow, spleen, and LNs in patients with AOSD than healthy controls, except for 18F-FDG uptake in the liver (p = 0.672) (Table 2). Representative PET/CT images of abnormal hypermetabolic distributions in two patients with AOSD are depicted in Fig. 1.
Bone marrow hypermetabolism occurred in 61.40% (35/57) of the patients, while spleen hypermetabolism occurred in 75.44% (43/57) of the patients. 66.7% (38/57) patients showed hypermetabolic LNs. In these cases, bilateral distribution of hypermetabolic LNs was commonly observed. The most commonly involved superficial LNs were cervical LNs (30/38, 78.95%) and axillary LNs (27/38, 71.05%), followed by inguinal LNs (13/38, 34.21%). Deep hypermetabolic LNs were also frequently observed, ranking with mediastinum LNs (19/38, 50.00%), pelvic LNs (16/38, 42.11%), retroperitoneal LNs (14/38, 36.84%), and abdominal LNs (8/38, 21.05%). In addition, four patients exhibited hypermetabolic LNs at the unilateral upper arm (intra-muscle).
Because of the limited scan range of PET/CT in this study, only the hip and shoulder joints were evaluated. Increased 18F-FDG uptake by the hip joints was only seen in one patient, with a SUVmax of 3.1, whereas three patients showed hypermetabolic shoulder joints with SUVmax of 3.1–3.3.
Abnormal FDG uptake by the right subscapularis was observed in only one patient. There was no increased 18F-FDG uptake by the skin in our cohort. In addition, there was only one (1.75%) patient in our cohort who showed parenchymal lung involvement with a SUVmax of 2.2 on the lung.
Correlations between laboratory findings, systemic score, and PET/CT parameters
The correlations between disease severity-related laboratory findings and PET/CT parameters are summarized in Table S1. The spleen hypermetabolism showed positive correlations with ferritin (rho = 0.478, p < 0.001), lactate dehydrogenase (LDH) (rho = 0.462, p < 0.001), and interleukin-2 receptor (IL-2R) (rho = 0.454, p = 0.002). The SUVmax of the LN negatively correlated with the white blood cell count (rho = − 0.500, p = 0.001). The MLVtotal of LNs displayed a positive correlation with the levels of IL-2R (rho = 0.495, p = 0.004).
The systemic score is a widely used indicator of AOSD disease severity. The SUVmax of the spleen (rho = 0.450, p < 0.001), SUVmax of the bone marrow (rho = 0.376, p = 0.004), TLGtotal (rho = 0.386, p = 0.017), and MLVtotal (rho = 0.391, p = 0.015) of LNs showed significant correlations with the systemic score (Fig. 2). Notably, the patients with systemic score ≥ 7 showed significantly higher SUVmax of the bone marrow (p = 0.005), spleen (p = 0.002), TLGtotal (p = 0.044), and MLVtotal (p = 0.033) of LNs than those with systemic score < 7 (Table S2).
PET/CT features in MAS
Eight of 57 patients were diagnosed with MAS during the current hospitalization and underwent PET/CT scanning before the diagnosis of MAS. The time interval between PET/CT scanning and the diagnosis of MAS ranged from 2 to 33 days (Table S3). Only two patients showed cytopenia involving cells of 1 lineage, suggesting MAS might be in development when performing PET/CT.
Patients with MAS showed significantly higher SUVmax of the spleen (p = 0.017, n = 8), TLGtotal (p = 0.045, n = 5), and MLVtotal of LNs (p = 0.012, n = 5) compared to patients without MAS (Table 3). Considering the high mortality rate of MAS, the risk factors for MAS were assessed (Table 4). ROC analyses revealed that MLVtotal of LNs > 62.2, SUVmax of the spleen > 3.55, and TLGtotal of LNs > 248,040 were the optimal cut-points for discriminating MAS occurrence. Elevated levels of LDH, IL-2R, platelet count, TLGtotal of LNs > 248,040, SUVmax of the spleen > 3.55, and MLVtotal of LNs > 62.2 significantly associated with MAS occurrence in univariate analysis. Subsequent multivariate analyses indicated that elevated IL-2R level and MLVtotal of LNs > 62.2 were independent risk factors of MAS.
MLVtotal of LNs > 62.2 had a sensitivity of 80.0%, a specificity of 93.9%, and an AUC of 0.855 in predicting MAS occurrence, which were comparable with those of the traditional marker, IL-2R (sensitivity, 75.0%; specificity, 97.3%; AUC, 0.899). Level of ferritin, SUVmax of the spleen, and the systemic score showed low specificity of 40.8–55.1% and AUC of 0.642–0.765 (Table 5).
18F-FDG PET/CT is a useful tool to rule out malignancy before the diagnosis of AOSD. The additional values including the potential correlations between the PET/CT findings and disease assessments are worth to be investigated. Hypermetabolic status of the bone marrow, spleen, and LNs in patients with AOSD have been observed in other studies [11,12,13,14, 22,23,24,25]. We further explored the utility of PET/CT to assess disease severity and MAS occurrence of AOSD. Our study suggests that PET/CT could act as an early detective tool to evaluate the disease severity and predict the occurrence of deadly complication, MAS, in patients with AOSD.
The glucose metabolic level of the spleen could be an effective indicator of AOSD severity. In the present study, not only positive correlations between SUVmax of the spleen and those laboratory profiles of AOSD including the levels of LDH, ferritin, and IL-2R were observed, but also the hypermetabolism of the spleen showed a higher correlation coefficient with systemic score and more frequent occurrence in patients with AOSD than those of the bone marrow and LNs. We assumed that the enhanced FDG accumulation in the spleen may be secondary to inflammation and hypercytokinemia . Furthermore, compared to the bone marrow and LNs, the spleen as a morphologically oblate and single organ is more likely to be drawn in a VOI on PET/CT images for more reliably and easily measuring its glucose metabolic levels in clinical practice. Additionally, a negative correlation between SUVmax of LNs and the white blood cell count was observed, which might be due to virus infection which was known as a trigger to AOSD [27,28,29], thereby temporarily disrupting the homeostasis of the bone marrow . Although both elevated  and reduced  18F-FDG uptake of the liver was observed in previous case reports, there was no statistical difference of SUVmax of the liver between patients with AOSD and healthy controls in our study. Similar to the studies of Dong et al.  and Jiang et al. , the prevalence of articular involvement in our cohort (10.53%) was low, indicating that increased 18F-FDG uptake in the joints may not be significant in patients with AOSD, which was probably due to the relatively mild involvement of joints in Chinese patients .
MLVtotal of LNs was a promising useful predictor of MAS occurrence in patients with AOSD. Previous studies have reported that 18F-FDG PET/CT-derived quantitative parameters were associated with the occurrence  or outcome of MAS [34, 35]. In our study, we further found that MLVtotal of LNs had better diagnostic performance than SUVmax of the spleen and TLGtotal of LNs for distinguishing patients with MAS from those without MAS. Hypermetabolism of whole-body LNs is believed to be due to the high accumulation of inflammatory cells and the over-activation of the mononuclear phagocyte system in LNs [34, 36]. Patients with MLVtotal of LNs > 62.2 showed a 20-fold increased risk for MAS occurrence than those with MLVtotal of LNs less than or equal to 62.2. IL-2R may serve as a good laboratory indicator of MAS . In this study, the IL-2R level was also an independent factor for predicting MAS occurrence with comparably high likelihood ratios to MLVtotal of LNs. Even if the predictive value of IL-2R, as a cheaper test than PET/CT, was already high, the value of PET/CT was not negligible. Firstly, the performance of IL-2R for diagnosing MAS is still limited [38, 39], especially with low specificity (38.8–72.5%). Secondly, PET/CT has been routinely used to rule out malignancy in the diagnosis of AOSD. Its evaluation of the MAS occurrence could bring additional diagnostic information to clinicians. We believe that the combined diagnosis of IL-2R and MLVtotal of LNs may have better predictive performance for MAS, but this conclusion cannot be obtained due to the limited number of samples in the present study. Therefore, further attention should be given to the whole-body inflammatory burden reflected by the MLVtotal of LNs for assessing the risk of MAS in patients with AOSD. Recently, the negative prognostic impact of lung involvement in patients with AOSD has been reported , and the lung involvement has been correlated with the occurrence of MAS [41, 42]. However, due to the low prevalence of parenchymal lung involvement in our study, no inferential analysis was made.
There are still some limitations in this study. (a) The number of cases with MAS was low (n = 8) leading to the possibility of model overfitting. (b) Short-term glucocorticoid treatment before PET/CT scanning in some patients may have a limited effect on the accuracy of SUV measurement . (c) There was a short time interval between PET/CT and diagnosis of MAS in two patients, whereas other six patients were diagnosed with MAS 1 week after PET/CT scan. Therefore, PET/CT may act as an actual display of the current disease activity as well as a prediction over time. This study was a pilot study at a single center, and more high evidence studies are needed to verify the reliability of the PET/CT parameters in the assessment of disease severity and the prediction of MAS in patients with AOSD.
Our data revealed that the glucose metabolic level of the spleen could be an effective and easy-to-use imaging indicator of disease severity, and MLVtotal of LNs > 62.2 was a strong predictor of MAS occurrence in patients with AOSD.
Availability of data and materials
All data generated or analyzed during this study are included in this published article and its supplementary information files.
- 18F-FDG PET/CT:
18F-fluorodeoxyglucose positron emission tomography/computed tomography
Adult-onset Still’s disease
Area under the curve
Conventional synthetic disease-modifying anti-rheumatic drugs
Erythrocyte sedimentation rate
Macrophage activation syndrome
- MLVtotal :
Total metabolic lesion volume
Magnetic resonance imaging
Volume of interest
Nonsteroidal anti-inflammatory drugs
- SUVmax :
Maximal standardized uptake value
Metabolic tumor volume
Total lesion glycolysis
Tumor necrosis factor
- TLGtotal :
Total lesion glycolysis
Receiver operating characteristic curve
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This work was supported by grants from Shanghai Jiao Tong University Medicine and Engineering Interdisciplinary Funding (YG2017MS61, YG2017QN58, and ZH2018QNA47), Shanghai Municipal Key Clinical Specialty (shslczdzk03403), and Shanghai Pujiang Program (18PJD030).
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Correlations between disease severity-related laboratory findings, systemic score, and PET/CT parameters. Table S2. Comparison of PET/CT parameters between patients with systemic score ≥ 7 and patients with systemic score <7. Table S3. Characteristic of eight patients with MAS.
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Wan, L., Gao, Y., Gu, J. et al. Total metabolic lesion volume of lymph nodes measured by 18F-FDG PET/CT: a new predictor of macrophage activation syndrome in adult-onset Still’s disease. Arthritis Res Ther 23, 97 (2021). https://doi.org/10.1186/s13075-021-02482-2