- Research article
- Open Access
Longitudinal immune cell monitoring identified CD14++ CD16+ intermediate monocyte as a marker of relapse in patients with ANCA-associated vasculitis
Arthritis Research & Therapy volume 22, Article number: 145 (2020)
Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is an autoimmune disease that affects small- to medium-sized blood vessels. Despite treatments having been improved, patients often experience disease relapses. It remains unclear how the immune cells involve in the development of vasculitis and how they fluctuate over the course of treatment. In this study, we aimed to identify the immune subsets and serum cytokines associated with disease relapse by comprehensive immuno-phenotyping in AAV patients.
We reviewed consecutive patients (n = 29) from Keio University Hospital who had been newly diagnosed with AAV from January 2015 to February 2019 and chronologically followed until 52 weeks. Numbers of circulating T cells, B cells, monocytes, and granulocytes were analyzed by flow cytometry (FACS). Serum levels of cytokines were measured by electrochemiluminescence enzyme immunoassay. Clinical information was obtained from patients’ records and association with time-course changes in immuno-phenotypes and serum levels of cytokines were assessed.
Comprehensive immuno-phenotyping data from 161 samples from 29 AAV patients at diagnosis; at weeks 4, 12, 24, and 52 of treatment; and at time of major relapse were examined. FACS analysis from patients with relapse revealed that CD14++ CD16+ intermediate monocytes and plasma cells concomitantly changed associated with disease relapse, which were independent from treatment regimen, ANCA status, or disease phenotype. In particular, the number of CD14++ CD16+ intermediate monocytes at relapse was significantly higher than that in remission or in healthy controls. Serum cytokine measurement revealed that changes of monocyte-derived proinflammatory cytokines such as IL-1β, IL-6, IL-8, and TNF-α were associated with disease status.
Chronological changes in CD14++ CD16+ intermediate monocyte counts can be a marker of disease relapse in AAV patients.
Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is an autoimmune disease that affects small- to medium-sized blood vessels and causes vascular inflammation and multiple organ damage [1, 2]. AAV is primarily managed with high-dose glucocorticoid in combination with cyclophosphamide, rituximab, or other conventional disease-modifying anti-rheumatic drugs (csDMARDs) [3,4,5]. Despite improvements following the introduction of cyclophosphamide- or rituximab-based treatment, however, patients often experience disease relapses [4,5,6,7].
Several lines of evidence from genetic and clinical research have revealed immune cells related to AAV pathogenesis. The results of genome-wide association studies suggest that human leukocyte antigen (HLA) genes including both major histocompatibility (MHC) class I and class II alleles are associated with disease susceptibility of AAV [8, 9]. Previous reports have shown abnormalities related to innate and adaptive immune cells are involved in the pathogenesis of AAV. For example, antigen-specific T and B cells are expanded, which produce ANCA [10,11,12]. ANCA subsequently leads neutrophils and monocytes to exposure of proteinase 3 (PR3) and myeloperoxidase (MPO) on their surface, allowing their recognition of ANCA . Other reports have shown proliferation of circulating CD4+ T [14, 15], follicular helper T (Tfh)  and activated CD8+ T cells , and defects of regulatory T cells . Previous reports showed that serum calprotectin and urinary soluble CD163 could be potential biomarkers, however, the exact role of monocytes in AAV has been unclear [19, 20]. Recently, we reported a comprehensive analysis using immuno-phenotyping to reveal that AAV patients have combined features of antibody production, cytotoxic activity, and neutrocytosis/lymphocytopenia . Proportions of HLA-DR+ CD4+ T cells, HLA-DR+ CD8+ T cells, plasma cells, plasmablasts, CD14++ CD16+ intermediate monocytes, eosinophils, and neutrophils were increased in AAV compared to healthy controls (HCs) . Various studies exist; however, identifying the specific cell subsets that drive the disease relapse has been challenging.
Here, therefore, we aimed to identify the immune subsets and serum cytokines associated with disease relapse by comprehensive immuno-phenotyping in AAV patients.
Patients and methods
Patients and healthy controls
Consecutive patients with newly diagnosed AAV (n = 29) who visited Keio University Hospital and fulfilled the 2012 Revised International Chapel Hill Consensus Conference Nomenclature  for granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA) between January 2015 and February 2019 and HCs (n = 18) were enrolled.
All patients received glucocorticoid therapy at an initial dose equivalent to 0.6–1.0 mg prednisolone (PSL) per kilogram per day, which was tapered based on previously reported clinical trials [4, 5]. As induction therapy, cyclophosphamide, rituximab, or others were selected by the attending physician in routine practice. Intravenous cyclophosphamide pulse was given at 10–15 mg/kg for 2 to 4 weeks in 6 cycles, and rituximab was given at 375 mg/m2 body surface area per week for 4 cycles . Patients who received rituximab as induction therapy were usually treated with rituximab as maintenance therapy. Patients who received cyclophosphamide were treated with azathioprine, methotrexate, or tacrolimus as maintenance therapy.
We confirmed that HCs did not have any autoimmune disease, severe allergic disorder, malignancy, or infection.
This study was approved by the research ethics committee of Keio University School of Medicine (#20140335) and was conducted according to the Declaration of Helsinki. Informed consent was obtained from all participants.
Clinical information was obtained from the patients’ records, including organ involvement of the ear, nose, and throat (ENT); central nervous system (CNS); peripheral nervous system (PNS); and kidney and interstitial lung disease (ILD), and data from laboratory studies of erythrocyte sedimentation rate (ESR), white blood cell count, hemoglobin, platelet count, ANCA titer (by chemiluminescence enzyme immunoassay; CLEIA), rheumatoid factor (RF), IgG, and C-reactive protein (CRP) in serum. Patients were followed until 52 weeks of treatment, until they died or were withdrawn, and information on disease relapse was examined.
We evaluated the total Birmingham Vasculitis Activity Score (BVAS) and the components of BVAS for each organ involvement. Remission was defined as an absence of clinical disease activity, as indicated by a BVAS of 0 that was maintained for at least 2 months . Relapse was defined as the recurrence or new appearance of any disease activity, as reflected by the BVAS following disease remission requiring reinduction therapy . Relapse of ILD was defined as the reappearance of new bilateral ground-glass opacities on high-resolution computed tomography requiring treatment intensification . Severe infectious diseases were designated grade ≥ 3 based on the Common Terminology Criteria for Adverse Events V.4.0 .
Flow cytometry (FACS) analysis for immuno-phenotyping
We collected heparinized peripheral blood samples from patients with AAV at diagnosis; at weeks 4, 12, 24, and 52 of treatment; and at time of major relapse. Because corticosteroids alter the number of peripheral immune cells in peripheral blood , immuno-phenotyping was carried out prior to receipt of high-dose corticosteroids at baseline and relapse. FACS analysis was performed according to MIFlowCyt  and was conducted on LSRFortessa™ X-20 (BD Biosciences, NJ, USA) without delay after collecting samples. One hundred microliters of heparinized blood samples were stained with antibodies (Supplementary Fig. 1); then, the staining samples were fixed by (PhosflowTM Lyse/Fix buffer, BD, NJ, USA). We used Flow-count Fluorospheres (BECKMAN COULTER, CA, USA) to acquire standardized number of immune cells. Data were analyzed by 3 individual authors blinded to the sample details using FlowJo v.10.1 Software (Tree Star, Stanford University, CA, USA).
The phenotypes of immune cell subsets were defined based on the Human Immunology Project protocol . We modified the definition of plasmablast and plasma cell based on other references [29, 30], CD19+ CD27+ IgD− CD20− CD38+ CD138+ as plasmablast and CD19+ CD27+ IgD− CD20− CD38+ CD138++ as plasma cell. When we performed FACS analysis, micro-sized cells were gated out, instead of using a dead/live marker. Details of the gating strategy are shown in Supplementary Fig. 1.
Levels of interferon (IFN)-γ, interleukin (IL)-1β, IL-6, IL-8, IL-10, and tumor necrosis factor (TNF)-α in serum were determined using a commercial electrochemiluminescence assay (Meso Scale Discovery, MD, USA) according to the manufacturer’s protocol. Serum samples were stored at − 80 °C prior to assay.
All analyses were conducted using SPSS Statistics version 26.0 (IBM Corp., NY, USA), JMP version 14.0 (SAS Institute, NC, USA) or GraphPad Prism software version 8.0 (GraphPad, CA, USA). Continuous data are expressed as median (IQR), and categorical data as number and/or percentage. Descriptive statistics were used to summarize the data. Continuous variables were compared using the Mann-Whitney U test, and categorical variables using the chi-squared test. Longitudinal data was analyzed using generalized estimating equations (GEE) [31,32,33], Wilcoxon signed rank sum test, repeated measures analysis of variance (ANOVA), and post hoc test. For GEE, the number of peripheral immune cells were normalized using z-score. Then, univariate GEE association over time for normalized number of peripheral immune cells and levels of serum cytokines with BVAS was analyzed. P values less than 0.05 were considered significant.
Baseline characteristics, treatment, and therapeutic prognosis of patients
We collected FACS data and clinical profiles from 29 AAV patients who were followed longitudinally across a total of 161 visits. All patients were treated with glucocorticoid-based treatment. Ten patients (34%) were treated with cyclophosphamide and 8 (28%) were treated with rituximab. The other 11 patients (38%) were treated with PSL as monotherapy or in combination with csDMARDs (azathioprine, 9; methotrexate, 1). All patients received rituximab as maintenance therapy when they reached remission by using rituximab as induction therapy. When patients reached remission with cyclophosphamide induction, they received azathioprine, methotrexate, or tacrolimus as maintenance therapy. A small number of patients were unable to use immunosuppressive drugs as maintenance therapy due to severe infection or other adverse events.
Baseline characteristics, treatment, and therapeutic prognosis of the patients treated with each regimen and HCs are summarized in Tables 1 and 2. Patients treated without using cyclophosphamide or rituximab tended to be older (cyclophosphamide vs rituximab vs others: 68 [52–75] vs 61 [50–76] vs 79 [64–83] years), higher proportion of MPA (50 vs 50 vs 64%), and lower BVAS (12 [12–17] vs 9 [9–16] vs 10 [5–14]). Of the 29 patients, 9 patients (26%) experienced relapse during the 52-week follow-up period.
Changes in immuno-phenotyping associated with disease relapse
To assess changes in the numbers of peripheral immune cells at remission and at relapse phase, we longitudinally profiled the immuno-phenotyping data of 9 patients who achieved remission then relapsed. We followed the immuno-phenotyping data of these patients until 52 weeks of treatment and calculated the correlation score between the fluctuation in each immune cell and change in disease activity.
We next examined the longitudinal associations between normalized number of peripheral immune cells and BVAS (Table 3). Univariate GEE analysis showed that changes in BVAS were significantly associated with changes in CD14++ CD16+ intermediate monocytes (β = 0.82, 95%CI 0.25–1.4, P = 0.005), CD14++ CD16− classical monocytes (β = 0.61, 95%CI 0.018–1.2, P = 0.043), and eosinophils (β = 0.56, 95%CI 0.026–1.1, P = 0.040). Additionally, plasma cells (β = 0.54, 95%CI: − 0.037 to 1.1, P = 0.067) tended to be associated with total BVAS, while that of other cell subsets did not show any notable correlation.
Then, we compared absolute number of circulating immune cells among onset, 1st remission, relapse, and 2nd remission using Wilcoxon signed rank sum test. As shown in Table 4, we found that the number of plasma cell and eosinophil at onset was higher than that at remission. CD14++ CD16+ intermediate monocytes at relapse were higher than that at remission. The number of CD14++ CD16+ intermediate monocytes at relapse was 3.2 times greater than that in HCs (Fig. 1A-a). The number of plasma cells at relapse was comparable to those in remitted patients and HCs (Fig. 1B-a).
The difference among treatment regimens (Fig. 1A-b, B-b, C-b, and D-b) and disease phenotypes (Fig. 1A-c, B-c, C-c, and D-c) was analyzed by repeated measures ANOVA and post hoc Friedman test. There was no significant difference in changes of monocytes (Fig. 1A-b), CD14++ CD16+ intermediate monocytes (Fig. 1B-b), CD19+ B cells (Fig. 1C-b), and plasma cells (Fig. 1D-b) among treatment regimens. In addition, the levels of monocytes (Fig. 1A-c), CD14++ CD16+ intermediate monocytes (Fig. 1B-c), CD19+ B cells (Fig. 1C-c), and plasma cells (Fig. 1D-c) were also independent on ANCA serotype or disease phenotype.
We found that only 3 (33%) patients (cases 1, 2, 9) showed a return of MPO/PR3-ANCA at relapse, while the remaining 6 patients remained ANCA-negative at relapse (Table 5). Of the 3 patients with ANCA return at relapse, all were GPA with CNS involvement, and 2 (67%) were PR3-ANCA-positive.
Chronological changes in immuno-phenotyping in AAV patients without relapse
To assess the difference between patients with and without relapse on CD14++ CD16+ intermediate monocytes and plasma cells, cell numbers in 20 patients successfully treated without relapse were followed at diagnosis and at weeks 4, 12, 24, and 52 of treatment (Supplementary Fig. 2). Changes in PSL dose and BVAS were shown (Supplementary Fig. 2A and B). The number of CD14++ CD16+ intermediate monocytes in patients remained high compared with HCs after 52 weeks of treatment (Supplementary Fig. 2C). In contrast, the number of plasma cells decreased to normal levels as well as in HCs after treatment (Supplementary Fig. 2D).
Chronological changes in humoral factors in AAV patients with relapse
Given the significant correlation between the number of CD14++ CD16+ intermediate monocytes and disease activity, we evaluated serum levels of the proinflammatory cytokines associated with monocyte activation, namely IFN-γ, IL-1β, IL-6, IL-8, IL-10, and TNF-α. Results of univariate GEE analysis for longitudinal associations between serum cytokine levels and BVAS showed significant associations for BVAS and IL-1β (β = 1.0, 95%CI 0.57–1.5, P < 0.001), IL-6 (β = 0.89, 95%CI 0.56–1.2, P < 0.001), IL-8 (β = 0.60, 95%CI 0.10–1.1, P = 0.018), and TNF-α (β = 0.91, 95%CI 0.43–1.4, P < 0.001) (Table 3).
As shown, serum levels of IL-1β, IL-6, IL-8, and TNF-α concomitantly changed with disease state (Fig. 2A) compared to CRP and ANCA titers of the patients (Fig. 2B). Serum cytokine measurement revealed that changes of monocyte-derived proinflammatory cytokines such as IL-1β, IL-6, IL-8, and TNF-α were associated with disease state.
Correlation analysis among immune cell subsets, age, BVAS, and serum cytokines
Additionally, we analyzed the correlation for the number of representative immune cell subsets with age, BVAS, and serum cytokines. The age of patients and HCs was not matched in this study. To determine the effect of age, we examined the correlation between age and the number of representative immune cell subsets in AAV patients and HCs (Supplementary Fig. 4). No significant correlation was observed between subjects’ age and the number of cell subsets, such as monocytes (Supplementary Fig. 4A), CD14++ CD16+ intermediate monocytes (Supplementary Fig. 4B), CD19+ B cells (Supplementary Fig. 4C), and plasma cells (Supplementary Fig. 4D). Though the number of plasma cells seems to be increased in patients over 80 years, a previous report has shown that plasma cell counts are inversely correlated with age . Thus, age did not affect immuno-phenotyping data in this study. Then, we showed the correlation for baseline BVAS with monocytes (Supplementary Fig. 5A), CD14++ CD16+ intermediate monocytes (Supplementary Fig. 5B), CD19+ B cells (Supplementary Fig. 5C), and plasma cells (Supplementary Fig. 5D). The numbers of CD14++ CD16+ intermediate monocytes and CD19+ B cells were correlated with chest and nervous system components of BVAS, respectively. Correlations for cytokine levels with monocytes (Supplementary Fig. 6A), CD14++ CD16+ intermediate monocytes (Supplementary Fig. 6B), CD19+ B cells (Supplementary Fig. 6C), and plasma cells (Supplementary Fig. 6D) were shown. Though the correlations for serum cytokine levels with these cell subsets were not statistically significant, they tend to be longitudinally associated with disease activity.
This study examined baseline and chronological changes in comprehensive immuno-phenotyping in patients with AAV. We revealed that CD14++ CD16+ intermediate monocytes and plasma cells were strongly correlated with disease relapse. Changes in CD14++ CD16+ intermediate monocytes and plasma cells were more sensitive markers of disease relapse than changes in MPO/PR3-ANCA titer. In particular, the number of CD14++ CD16+ intermediate monocytes at relapse was significantly greater than that in remission or in HCs. Taken together, our data suggest that the number of peripheral CD14++ CD16+ intermediate monocytes may be useful for evaluating disease activity in AAV.
Although B cell count and ANCA titer increase are shown to be associated with relapse [35, 36], these are not recommended for monitoring treatment . Von Borstel et al. have reported CD27+ CD38hi B cell frequencies during remission were associated with the risk for relapse in GPA patients, suggesting more specific cell subsets may potentially be markers associated with disease relapse . We showed that changes in plasma cells were strongly correlated with the reactivation of CNS disease. This hypothesis is supported by our recent finding of a significant correlation between circulating B cells and the CNS component of BVAS [21, 38]. CNS lesion may be a good indicator of the need for B cell depletion therapy in AAV.
Here, we showed that changes in CD14++ CD16+ intermediate monocytes were remarkably associated with disease relapse in AAV. Although CD14++ CD16+ intermediate monocytes account for a relatively small fraction of total monocytes in peripheral blood (about 8% of monocytes in HC) , the proportion of CD14++ CD16+ intermediate monocytes is increased to 15% among total monocytes in AAV patients [21, 39]. We validated the former analyses [21, 39] and identified CD14++ CD16+ intermediate monocyte as the most useful marker of disease relapse in AAV using comprehensive immuno-phenotyping. Though CD14++ CD16+ intermediate monocytes expansion have been reported in other inflammatory diseases such as rheumatoid arthritis , van Sleen et al. reported that CD14++ CD16− classical monocytes expanded in GCA rather than CD14++ CD16+ intermediate monocytes . Our recent report revealed that LVV disease activity was not consistent with changes of CD14++ CD16+ intermediate monocytes . In combination with findings that monocytes and macrophages are frequently found in vascular infiltrates of AAV patients , CD14++ CD16+ intermediate monocytes may play a characteristically important role in the pathogenesis of AAV. Interestingly, the number of CD14++ CD16+ intermediate monocytes remained elevated in AAV patients who had achieved remission (Fig. 1B and Supplementary Fig. 2C). Considering the elevated level of CD14++ CD16+ intermediate monocytes was independent of the treatment regimen, the existing treatments might not be sufficient to achieve deep and stable remission state in AAV. Our findings raise the possibilities that pathogenic CD14++ CD16+ intermediate monocytes are resistant to the current treatments and controlling the functions of this cell subset may lead to reduction of relapse in AAV. Our study suggests that CD14++ CD16+ intermediate monocytes may be one of the novel therapeutic cell targets against AAV.
While the upstream of CD14++ CD16+ intermediate monocytes expansion or their molecular effect in AAV pathogenesis is beyond the scope of this manuscript, CD16, a receptor for immunoglobin gamma Fc region III, may have a key role in the pathogenesis of AAV. The proportion of CD16 on intermediate monocytes correlates with MPO expression on these cells , providing an insight into the possible activation of these cells by MPO-ANCA. ANCA has been shown to stimulate oxygen radical production and to produce inflammatory cytokines from monocytes [45, 46]. We found that the levels of IL-1β, IL-6, IL-8, and TNF-α were associated with disease activity. CD14++ CD16+ intermediate monocyte is the most proinflammatory monocyte subset and also involved in cell differentiation, including development from naive lymphocyte into Th17 . Considering major sources of these cytokines are activated monocytes and macrophages, CD14++ CD16+ intermediate monocytes are key effectors in AAV pathogenesis through the production of proinflammatory cytokines.
Our study had several limitations. First, this is a study with a small sample size and with a short observation period. Second, treatment was not randomized and depends on the attending physician. Third, our cohort included relatively less kidney recurrence, whereas previous trials such as RITUXVAS and RAVE mainly focused on renal vasculitis [4, 5]. Last, we did not show that the changes in CD14++ CD16+ intermediate monocytes were specific for AAV because we did not have disease controls. For these reasons, this report represented a pilot study that was designed for discovery analysis and which had inadequate power to incorporate an adjustment for multivariate analysis. Further studies of additional immuno-phenotyping evidence are required to strengthen our findings and make it possible to predict disease relapse in AAV.
Our findings demonstrate CD14++ CD16+ intermediate monocyte counts reflect the disease activity of AAV and will aid to distinguish active patients from those in remission. Chronological changes in CD14++ CD16+ intermediate monocyte counts can be a marker of disease relapse in AAV patients.
Availability of data and materials
All data generated and analyzed in this study are disclosed.
Anti-neutrophil cytoplasmic antibody
Conventional disease-modifying anti-rheumatic drugs
Human leukocyte antigen
Follicular helper T
Granulomatosis with polyangiitis
Ear, nose and throat
Central nervous system
Peripheral nervous system
Interstitial lung disease
Chemiluminescent enzyme immunoassay
Birmingham Vasculitis Activity Score
Tumor necrosis factor
Large vessel vasculitis
Fauci AS, Wolff SM. Wegener’s granulomatosis: studies in eighteen patients and a review of the literature. Medicine (Baltimore). 1973;52:535–61.
Jennette JC, Falk RJ, Andrassy K, Bacon PA, Churg J, Gross WL, et al. Nomenclature of systemic vasculitis. Proposal of an international consensus conference. Arthritis Rheum. 1994;37:187–92.
Yates M, Watts RA, Bajema IM, Cid MC, Crestani B, Hauser T, et al. EULAR/ERA-EDTA recommendations for the management of ANCA-associated vasculitis. Ann Rheum Dis. 2016;75:1583–94.
Jones RB, Tervaert JW, Hauser T, Luqmani R, Morgan MD, Peh CA, et al. Rituximab versus cyclophosphamide in ANCA-associated renal vasculitis. N Engl J Med. 2010;363:211–20.
Stone JH, Merkel PA, Spiera R, Seo P, Langford CA, Hoffman GS, et al. Rituximab versus cyclophosphamide for ANCA-associated vasculitis. N Engl J Med. 2010;363:221–32.
Flossmann O, Berden A, de Groot K, Hagen C, Harper L, Heijl C, et al. Long-term patient survival in ANCA-associated vasculitis. Ann Rheum Dis. 2011;70:488–94.
Alberici F, Smith RM, Jones RB, Roberts DM, Willcocks LC, Chaudhry A, et al. Long-term follow-up of patients who received repeat-dose rituximab as maintenance therapy for ANCA-associated vasculitis. Rheumatology (Oxford). 2015;54:1153–60.
Tsuchiya N, Kobayashi S, Kawasaki A, Kyogoku C, Arimura Y, Yoshida M, et al. Genetic background of Japanese patients with antineutrophil cytoplasmic antibody-associated vasculitis: association of HLA-DRB1*0901 with microscopic polyangiitis. J Rheumatol. 2003;30:1534–40.
Lyons PA, Rayner TF, Trivedi S, Holle JU, Watts RA, Jayne DR, et al. Genetically distinct subsets within ANCA-associated vasculitis. N Engl J Med. 2012;367:214–23.
Chavele KM, Shukla D, Keteepe-Arachi T, Seidel JA, Fuchs D, Pusey CD, Salama AD. Regulation of myeloperoxidase-specific T cell responses during disease remission in antineutrophil cytoplasmic antibody-associated vasculitis: the role of Treg cells and tryptophan degradation. Arthritis Rheum. 2010;62:1539–48.
Cornec D, Berti A, Hummel A, Peikert T, Pers JO, Specks U. Identification and phenotyping of circulating autoreactive proteinase 3-specific B cells in patients with PR3-ANCA associated vasculitis and healthy controls. J Autoimmun. 2017;84:122–31.
Free ME, Stember KG, Hess JJ, McInnis EA, Lardinois O, Hogan SL, et al. Identification and phenotyping of circulating autoreactive proteinase 3-specific B cells in patients with PR3-ANCA associated vasculitis and healthy controls. J Autoimmun. 2020;106:102306.
Hess C, Sadallah S, Schifferli JA. Induction of neutrophil responsiveness to myeloperoxidase antibodies by their exposure to supernatant of degranulated autologous neutrophils. Blood. 2000;96:2822–7.
Berden AE, Kallenberg CG, Savage CO, Yard BA, Abdulahad WH, de Heer E. Cellular immunity in Wegener's granulomatosis: characterizing T lymphocytes. Arthritis Rheum. 2009;60:1578–87.
Abdulahad WH, Lamprecht P, Kallenberg CG. T-helper cells as new players in ANCA-associated vasculitides. Arthritis Res Ther. 2011;13:236.
Abdulahad WH, Lepse N, Stegeman CA, Huitema MG, Doornbos-van der Meer B, Tadema H, et al. Increased frequency of circulating IL-21 producing Th-cells in patients with granulomatosis with polyangiitis (GPA). Arthritis Res Ther. 2013;15:R70.
Iking-Konert C, Vogl T, Prior B, Wagner C, Sander O, Bleck E, et al. T lymphocytes in patients with primary vasculitis: expansion of CD8+ T cells with the propensity to activate polymorphonuclear neutrophils. Rheumatology (Oxford). 2008;47:609–16.
Abdulahad WH, Stegeman CA, Van der Geld YM, Doornbos-van der Meer B, Limburg PC, Kallenberg CG. Functional defect of circulating CD4+ T cells in patients with Wegener’s granulomatosis in remission. Arthritis Rheum. 2007;56:2080–91.
O'Reilly VP, Wong L, Kennedy C, Elliot LA, O'Meachair S, Coughlan AM, et al. Urinary soluble CD163 in active renal vasculitis. J Am Soc Nephrol. 2016;27:2906–16.
Pepper RJ, Draibe JB, Caplin B, Fervenza FC, Hoffman GS, Kallenberg CG, et al. Association of serum calprotectin (S100A8/A9) level with disease relapse in proteinase 3-antineutrophil cytoplasmic antibody-associated vasculitis. Arthritis Rheumatol. 2017;69:185–93.
Matsumoto K, Suzuki K, Yoshimoto K, Seki N, Tsujimoto H, Chiba K, Takeuchi T. Significant association between clinical characteristics and immuno-phenotypes in patients with ANCA-associated vasculitis. Rheumatology (Oxford). 2019;59:545–53.
Jennette JC, Falk RJ, Bacon PA, Basu N, Cid MC, Ferrario F, et al. 2012 revised International Chapel Hill Consensus Conference Nomenclature of Vasculitis. Arthritis Rheum. 2013;65:1–11.
Stone JH, Hoffman GS, Merkel PA, Min YI, Uhlfelder ML, Hellmann DB, et al. A disease-specific activity index for Wegener's granulomatosis: modification of the Birmingham Vasculitis Activity Score. International Network for the Study of the Systemic Vasculitides (INSSYS). Arthritis Rheum. 2001;44:912–20.
Collard HR, Moore BB, Flaherty KR, Brown KK, Kaner RJ, King TE Jr, et al. Acute exacerbations of idiopathic pulmonary fibrosis. Am J Respir Crit Care Med. 2007;176:636–43.
National Cancer Institute. Common terminology Criteria for Adverse Events (CtCAE) Version 4.0. 2009 https://evs.nci.nih.gov/ftp1/CtCAE/CtCAE_4.03_2010-06-14_Quickreference_5x7.pdf.
Rogacev KS, Zawada AM, Hundsdorfer J, Achenbach M, Held G, Fliser D, Heine GH. Immunosuppression and monocyte subsets. Nephrol Dial Transplant. 2015;30:143–53.
Lee JA, Spidlen J, Boyce K, Cai J, Crosbie N, Dalphin M, et al. MIFlowCyt: the minimum information about a flow cytometry experiment. Cytometry A. 2008;73:926–30.
Maecker HT, McCoy JP, Nussenblatt R. Standardizing immunophenotyping for the human immunology project. Nat Rev Immunol. 2012;12:191–200.
Marinos C Dalakas. B cells as therapeutic targets in autoimmune neurological disorders. Nature Clinical Practice Neurology. 2008;4:557–67.
Hiepe F, Radbruch A. Plasma cells as an innovative target in autoimmune disease with renal manifestations. Nat Rev Nephrol. 2016;12:232–40.
Liang KY, Zeger SL. Longitudinal data analysis using generalized linear models. Biometrika. 1986;73:13–22.
Zeger SL, Liang KY. The analysis of discrete and continuous longitudinal data. Biometrics. 1986;42:121–30.
Louthrenoo W, Kasitanon N, Morand E, Kandane-Rathnayake R. Comparison of performance of specific (SLEQOL) and generic (SF36) health-related quality of life questionnaires and their associations with disease status of systemic lupus erythematosus: a longitudinal study. Arthritis Res Ther. 2020;22:8.
Caraux A, Klein B, Paiva B, et al. Circulating Human B and Plasma Cells. Age-associated changes in counts and detailed characterization of circulating normal CD138- And CD138+ plasma cells. Hematologica. 2010;95:1016–20.
Thai LH, Charles P, Resche-Rigon M, Desseaux K, Guillevin L. Are anti-proteinase-3 ANCA a useful marker of granulomatosis with polyangiitis (Wegener’s) relapses? Results of a retrospective study on 126 patients. Autoimmun Rev. 2014;13:313–8.
Watanabe H, Sada KE, Matsumoto Y, Harigai M, Amano K, Dobashi H, et al. Association between reappearance of myeloperoxidase-antineutrophil cytoplasmic antibody and relapse in antineutrophil cytoplasmic antibody-associated vasculitis: subgroup analysis of nationwide prospective cohort studies. Arthritis Rheumatol. 2018;70:1626–33.
von Borstel A, Land J, Abdulahad WH, Rutgers A, Stegeman CA, Diepstra A, et al. CD27+ CD38hi B cell frequency during remission predicts relapsing disease in granulomatosis with polyangiitis patients. Front Immunol. 2019;10:2221.
Matsumoto K, Akiyama M, Kajio N, Otomo K, Suzuki K, Nishina N, et al. Adolescent PR3-ANCA-positive hypertrophic pachymeningitis: a case report and review of the literature. Medicine (Baltimore). 2018;97:e0521.
O'Brien EC, Abdulahad WH, Rutgers A, Huitema MG, O'Reilly VP, Coughlan AM, et al. Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1β secretion in response to anti-MPO antibodies. Sci Rep. 2015;5:11888.
Tsukamoto M, Seta N, Yoshimoto K, Suzuki K, Yamaoka K, Takeuchi T. CD14bright CD16+ intermediate monocytes are induced by interleukin-10 and positively correlate with disease activity in rheumatoid arthritis. Arthritis Res Ther. 2017;19:28.
van Sleen Y, Wang Q, van der Geest KSM, Westra J, Abdulahad WH, Heeringa P, et al. Involvement of monocyte subsets in the immunopathology of giant cell arteritis. Sci Rep. 2017;7:6553.
Matsumoto K, Suzuki K, Yoshimoto K, Seki N, Tsujimoto H, Chiba K, Takeuchi T. Significant association between clinical characteristics and changes in peripheral immuno-phenotype in large vessel vasculitis. Arthritis Res Ther. 2019;21:304.
de Souza AWS, van Timmeren M, Sanders JS, et al. M2 macrophage is the predominant phenotype in airways inflammatory lesions in patients with granulomatosis with polyangiitis. Arthritis Res Ther. 2017;19:100.
Jennette JC, Falk RJ, Hu P, Xiao H. Pathogenesis of antineutrophil cytoplasmic autoantibody-associated small-vessel vasculitis. Annu Rev Pathol. 2013;8:139–60.
Ralston DR, Marsh CB, Lowe MP, Wewers MD. Antineutrophil cytoplasmic antibodies induce monocyte IL-8 release. Role of surface proteinase-3, alpha1-antitrypsin, and Fcgamma receptors. J Clin Invest. 1997;100:1416–24.
Weidner S, Neupert W, Goppelt-Struebe M, Rupprecht HD. Antineutrophil cytoplasmic antibodies induce human monocytes to produce oxygen radicals in vitro. Arthritis Rheum. 2001;44:1698–706.
Rossol M, Kraus S, Pierer M, Baerwald C, Wagner U. The CD14(bright) CD16+ monocyte subset is expanded in rheumatoid arthritis and promotes expansion of the Th17 cell population. Arthritis Rheum. 2012;64:671–7.
We are very grateful to Ms. Yumi Ikeda, Ms. Yuko Takaishi, and Ms. Kumiko Tanaka for their technical support.
This study was financially supported by Mitsubishi Tanabe Pharma Corporation and Keio University School of Medicine.
Ethics approval and consent to participate
This study was approved by the research ethics committee of the Keio University School of Medicine (#20140335) and was conducted according to the Declaration of Helsinki.
Consent for publication
Informed consent was obtained from all patients and HCs.
KM, KS, and KY have no competing interests to disclose. NS, HT, and KC were employees of Mitsubishi Tanabe Pharma Corporation. TT has received research grants from Mitsubishi Tanabe Pharma Corporation.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Additional file 2: Supplementary Fig. 2. Chronological changes in immuno-phenotyping in AAV patients without relapse. Changes in (A) PSL dose, (B) BVAS, (C) CD14++ CD16+ intermediate monocytes and (D) plasma cells in AAV patients without relapse (n = 20). Lines showed the median (IQR) number of immune cell subsets in healthy controls.
Additional file 3: Supplementary Fig. 3. Changes in humoral factors according to treatment, ANCA serotype or disease subtype. Serum levels of (A) IFN-γ, (B) IL-1β, (C) IL-6, (D) IL-8, (E) IL-10, (F) TNF-α, (G) CRP and (H) ANCA titer in patients with relapse (n = 9). (A-F) Lines showed the median (IQR) level of cytokines in healthy controls. Difference among treatment regimens (A-b, B-b, C-b, D-b, E-b, F-b, G-b and H-b) and disease phenotypes (A-c, B-c, C-c, D-c, E-c, F-c, G-c and H-c) were analyzed by repeated measures ANOVA and post-hoc Friedman test.
Additional file 4: Supplementary Fig. 4. Correlation between age and number of representative immune cell subsets. Correlation between age and the number of (A) monocytes, (B) CD14++ CD16+ intermediate monocytes, (C) CD19+ B cells and (D) plasma cells. (A-a, B-a, C-a, D-a) HC; (A-b, B-b, C-b, D-b) AAV. Pearson’s correlation coefficient was used.
Additional file 5: Supplementary Fig. 5. Correlation for baseline BVAS with representative immune cell subsets. Correlation for baseline BVAS with (A) monocytes, (B) CD14++ CD16+ intermediate monocytes, (C) CD19+ B cells and (D) plasma cells. (a) Total BVAS, and components of BVAS for (b) ear, nose, throat, (c) nervous system, (d) renal and (e) chest were shown. Pearson’s correlation coefficient was used.
Additional file 6: Supplementary Fig. 6. Correlations for cytokine levels with representative immune cell subsets. Correlations for cytokine levels with (A) monocytes, (B) CD14++ CD16+ intermediate monocytes, (C) CD19+ B cells and (D) plasma cells. Correlations for each cytokine of (a) IFN-γ, (b) IL-1β, (c) IL-6, (d) IL-8, (e) IL-10 and (f) TNF-α are shown. Pearson’s correlation coefficient was used.
About this article
Cite this article
Matsumoto, K., Suzuki, K., Yoshimoto, K. et al. Longitudinal immune cell monitoring identified CD14++ CD16+ intermediate monocyte as a marker of relapse in patients with ANCA-associated vasculitis. Arthritis Res Ther 22, 145 (2020). https://doi.org/10.1186/s13075-020-02234-8
- Anti-neutrophil cytoplasmic antibody-associated vasculitis
- Intermediate monocyte
- Plasma cell